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AP192F

Sigma-Aldrich

Donkey Anti-Mouse IgG Antibody, FITC conjugate, Species Adsorbed

Chemicon®, from donkey

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eCl@ss:
32160702
NACRES:
NA.46

biological source

donkey

Quality Level

conjugate

FITC conjugate

antibody form

affinity purified immunoglobulin

antibody product type

secondary antibodies

clone

polyclonal

species reactivity

mouse

manufacturer/tradename

Chemicon®

technique(s)

immunofluorescence: suitable

shipped in

wet ice

target post-translational modification

unmodified

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AP180FAP192CAP192R
conjugate

FITC conjugate

conjugate

FITC conjugate

conjugate

CY3 conjugate

conjugate

TRITC conjugate, rhodamine conjugate

biological source

donkey

biological source

donkey

biological source

donkey

biological source

donkey

species reactivity

mouse

species reactivity

goat

species reactivity

mouse

species reactivity

mouse

technique(s)

immunofluorescence: suitable

technique(s)

immunofluorescence: suitable

technique(s)

immunofluorescence: suitable

technique(s)

immunofluorescence: suitable

clone

polyclonal

clone

polyclonal

clone

polyclonal

clone

polyclonal

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General description

Immunoglobulin G (IgG), is one of the most abundant proteins in human serum with normal levels between 8-17 mg/mL in adult blood. IgG is important for our defence against microorganisms and the molecules are produced by B lymphocytes as a part of our adaptive immune response. The IgG molecule has two separate functions; to bind to the pathogen that elicited the response and to recruit other cells and molecules to destroy the antigen. The variability of the IgG pool is generated by somatic recombination and the number of specificities in an individual at a given time point is estimated to be 1011 variants.

Specificity

Specific for Mouse IgG.

Application

Detect Donkey Mouse IgG using this Donkey anti-Mouse IgG Antibody, FITC conjugate, Species Adsorbed validated for use in IF.
Research Category
Secondary & Control Antibodies
Research Sub Category
Secondary Antibodies Adsorbed for Dual Labeling
Suggested dilution for most applications: 1:50-1:200.

Optimal working dilutions must be determined by the end user.

Physical form

ImmunoAffinity Purified
Lyophilized. Buffer = 0.02 M Sodium Phosphate, 0.25 M NaCl, pH 7.6, with 15 mg/mL BSA and 0.1% sodium azide.

RECONSTITUTION:

Reconstitute to 1 mg/mL with sterile distilled water.

F/P RATIO:

15 micrograms FITC per mg of antibody.

Storage and Stability

Maintain lyophilized product at 2–8°C for up to 12 months. After reconstitution the product is stable for several weeks at 2–8°C as an undiluted liquid. For extended storage after reconstitution, add an equal volume of glycerol to make a final concentration of 50% glycerol followed by storage at -20°C in undiluted aliquots for up to 12 months. Please note the concentration of protein (and buffer salts) will decrease to one-half of the original after the addition of glycerol. Avoid repeated freeze/thaw cycles.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

hcodes

Hazard Classifications

Aquatic Chronic 3

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3


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