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DP02

Sigma-Aldrich

Anti-SV40 T Antigen (Ab-2) Mouse mAb (PAb416)

liquid, clone PAb416, Calbiochem®

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biological source

mouse

Quality Level

antibody form

purified antibody

antibody product type

primary antibodies

clone

PAb416, monoclonal

form

liquid

contains

≤0.1% sodium azide as preservative

species reactivity

SV40-infected cells

manufacturer/tradename

Calbiochem®

storage condition

do not freeze

isotype

IgG2a

shipped in

wet ice

storage temp.

2-8°C

target post-translational modification

unmodified

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DP02AMABF121OP143
vibrant-m

OP143

Anti-MDM2 (Ab-3) Mouse mAb (4B11)

biological source

mouse

biological source

mouse

biological source

mouse

biological source

mouse

species reactivity

SV40-infected cells

species reactivity

SV40-infected cells

species reactivity

SV40-infected cells

species reactivity

mouse, human

clone

PAb416, monoclonal

clone

PAb416, monoclonal

clone

PAb416, monoclonal

clone

4B11, monoclonal

Quality Level

100

Quality Level

100

Quality Level

100

Quality Level

100

shipped in

wet ice

shipped in

wet ice

shipped in

wet ice

shipped in

wet ice

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General description

Purified mouse monoclonal antibody generated by immunizing mice with the specified immunogen and fusing splenocytes with NS-1 mouse myeloma cells (see application references). Recognizes the ~94 kDa SV40 large T antigen.
Recognizes ~94 kDa SV40 large T antigen in SV80 and SV-T2 cells.
SV40 T Ag (Ab-2) is a mouse monoclonal antibody with specificity for antigenic determinants unique to the SV40 large T antigen and non-reactive with SV40 small T antigen. Both antigens are encoded by the early region of the SV40 genome. The large T antigen binds DNA, and complexes with a 53,000 dalton cellular transforming protein, p53, which is required for initiation of viral DNA replication during lytic growth. In addition the large T antigen binds DNA polymerase and the transcription factor AP-2 and forms a specific complex with the p105 product of the retinoblastoma susceptibility gene.
This Anti-SV40 T Antigen (Ab-2) Mouse mAb (PAb416) is validated for use in Affinity Purification, Immunoblotting, IF, IP, Paraffin Sections for the detection of SV40 T Antigen (Ab-2).

Immunogen

Epitope: Within amino acids 83-128
purified SV40 large T-antigen

application

Affinity Purification (use Cat. No. DP02A)

Immunoblotting (1-5 µg/ml, see application references)

Immunofluorescence (1-5 µg/ml)

Immunoprecipitation (1-5 µg antibody, use Cat. No. DP02A)

Paraffin Sections (see application references)

Packaging

Please refer to vial label for lot-specific concentration.

Warning

Toxicity: Standard Handling (A)

Physical form

In 50 mM sodium phosphate buffer, 0.2% gelatin, pH 7.5.

Analysis Note

Negative Control
3T3 cells
Positive Control
SV80 or SV-T2 cells

Other Notes

DeCaprio, J.A., et al. 1988. Cell54, 275.
Whyte, P., et al. 1988. Nature334, 124.
Mitchell, P.J., et al. 1987. Cell50, 847.
Dixon, R.A.F. and Nathans, D., 1985. J. Virol.53, 1001.
Simanis, V. and Lane, D.P. 1985. Virol.144, 88.
Mann, R.S. and Carroll, R.B. 1984. Virology138, 379.
Sarnow, P., et al. 1982. Cell28, 387.
Crawford, L.V., et al. 1981. Proc. Natl. Acad. Sci. USA78, 41.
Lane, D.P. and Crawford, L.V. 1979. Nature278, 261.
Carroll, R.B., et al. 1974. Proc. Natl. Acad. Sci. USA71, 3754.
Tooze, J. 1973. Cold Spring Harbor, New York.
Tegtmeyer, P. 1972. J. Virol.10, 591.
The agarose conjugate (Cat. No. DP02A) is also available and suitable for affinity purification and immunoprecipitation. Antibody should be titrated for optimal results in individual systems.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable


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Volker Nickeleit et al.
Journal of the American Society of Nephrology : JASN, 29(2), 680-693 (2017-12-28)
Polyomavirus nephropathy (PVN) is a common viral infection of renal allografts, with biopsy-proven incidence of approximately 5%. A generally accepted morphologic classification of definitive PVN that groups histologic changes, reflects clinical presentation, and facilitates comparative outcome analyses is lacking. Here
Archives of pathology & laboratory medicine, 142(9), e2-e202 (2018-09-13)
and case study poster sessions will be conducted during the 2018 College of American Pathologists Annual Meeting (CAP18), which is scheduled for October 20 to 24, 2018. The meeting will take place at the Hyatt Regency, Chicago, Illinois. The poster
V Krump-Konvalinkova et al.
Laboratory investigation; a journal of technical methods and pathology, 81(12), 1717-1727 (2001-12-14)
The limited lifespan of human microvascular endothelial cells in cell culture represents a major obstacle for the study of microvascular pathobiology. To date, no endothelial cell line is available that demonstrates all of the fundamental characteristics of microvascular endothelial cells.
Xu-Tao Chen et al.
Annals of translational medicine, 8(5), 235-235 (2020-04-21)
The positive predictive value (PPV) of urinary decoy cells for diagnosing BK polyomavirus associated-nephropathy (BKPyVAN) is low. This study was designed to increase the PPV of urinary decoy cells for diagnosing BKPyVAN in kidney transplant recipients. A total of 105
Mingxin Shi et al.
Biology of reproduction, 102(5), 1055-1064 (2020-01-14)
Ovarian cancer (OvCa) remains the most common cause of death from gynecological malignancies. Genetically engineered mouse models have been used to study initiation, origin, progression, and/or mechanisms of OvCa. Based on the clinical features of OvCa, we examined a quadruple

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