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SRP2093

Sigma-Aldrich

SV40 T-antigen from simian virus

recombinant, expressed in insect cells, ≥80% (SDS-PAGE)

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biological source

simian virus

recombinant

expressed in insect cells

assay

≥80% (SDS-PAGE)

form

frozen liquid

mol wt

~83.3 kDa

packaging

pkg of 5 μg

storage condition

avoid repeated freeze/thaw cycles

concentration

400 μg/mL

color

clear colorless

NCBI accession no.

UniProt accession no.

shipped in

dry ice

storage temp.

−70°C

Gene Information

simian virus ... SV40gp6(1489531)

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SV40 T-antigen from simian virus

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Rev-ErbA, β (RVR) human

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RAR, γ human

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SRP2094

Fos-related antigen 1 human

biological source

simian virus

biological source

human

biological source

human

biological source

human

recombinant

expressed in insect cells

recombinant

expressed in insect cells

recombinant

expressed in insect cells

recombinant

expressed in insect cells

UniProt accession no.

P03070

UniProt accession no.

Q14995

UniProt accession no.

P13631

UniProt accession no.

P15407

mol wt

~83.3 kDa

mol wt

~66.6 kDa

mol wt

~52.1 kDa

mol wt

~31.2 kDa

form

frozen liquid

form

frozen liquid

form

frozen liquid

form

frozen liquid

Biochem/physiol Actions

The large T antigen (TAg) of Simian virus 40 acts as an initiator of DNA replication, participates in cellular transformation, and induces cell growth. A number of functional domains have been characterized including the N-terminal J domain, nuclear localization signal (NLS), internal helicase domain, Rb and topoisomerase I interacting domain, p53 and ATP binding domain. The p53-interacting domain is also essential for binding p300/CBP. Phosphorylation of TAg by several kinases may regulate its function both positively and negatively.
The viral replication of simian virus 40 (SV40) is carried out by a single protein, T-antigen. This feature makes the virus a useful system for understanding the process of replication and to identify the components of replication machinery. It functions as a DNA helicase along with a cellular single-strand DNA binding protein and topoisomerase I or II, leading to extensive origin unwinding and formation of a pre-initiation complex. The T-antigen associates with two tumor suppressor proteins, p53 and the retinoblastoma protein pRb, and inactivates them.

Physical form

Clear and colorless frozen liquid solution

Preparation Note

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. While working, please keep sample on ice.

Storage Class

10 - Combustible liquids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable


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Interaction of DNA polymerase alpha-primase with cellular replication protein A and SV40 T antigen.
Dornreiter I
The Embo Journal, 11, 769-776 (1992)
Time-sensitive reversal of hyperplasia in transgenic mice expressing SV40 T antigen.
Ewald D
Science, 273, 1384-1386 (1996)
H H Chao et al.
Molecular and cellular biology, 20(20), 7624-7633 (2000-09-26)
At least three domains of simian virus 40 large T antigen (TAg) participate in cellular transformation. The LXCXE motif of TAg binds to all members of the retinoblastoma protein (pRB) family of tumor suppressors. The N-terminal 70 residues of TAg
Simian virus 40 large T antigen: the puzzle, the pieces, and the emerging picture.
E Fanning
Journal of virology, 66(3), 1289-1293 (1992-03-01)
Z Q Pan et al.
The Journal of biological chemistry, 271(36), 22111-22116 (1996-09-06)
The replication of simian virus 40 (SV40) DNA in vitro requires a trimeric single-stranded DNA (ssDNA)-binding protein called HSSB or RPA. HSSB supports the unwinding of DNA containing the SV40 origin in the presence of the viral-encoded T antigen and

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