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GF101

Sigma-Aldrich

CD40 Ligand/TRAP Protein, Recombinant human

The CD40 Ligand (CD40L/TRAP protein) is a type II membrane protein which, effectuates the helper function of T cells on resting B cells.

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Synonym(s):
CD154, TRAP, TNF-related activation protein, T cell antigen Gp39
eCl@ss:
32160405
NACRES:
NA.75

biological source

human

Quality Level

Assay

>98% (SDS-PAGE and HPLC)

manufacturer/tradename

Chemicon®

technique(s)

cell culture | mammalian: suitable

impurities

<0.1 ng/μg Endotoxin (of sCD40L/TRAP; 1EU/μg)

NCBI accession no.

UniProt accession no.

shipped in

dry ice

Gene Information

human ... CD40LG(959)

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Sigma-Aldrich

Sigma-Aldrich

SRP8044

CD40L, Soluble human

assay

>98% (SDS-PAGE and HPLC)

assay

>95% (SDS-PAGE)

assay

-

assay

-

technique(s)

cell culture | mammalian: suitable

technique(s)

-

technique(s)

-

technique(s)

indirect ELISA: suitable, western blot: 1-5 μg/mL

impurities

<0.1 ng/μg Endotoxin (of sCD40L/TRAP; 1EU/μg)

impurities

<0.01 EU/μg endotoxin, tested

impurities

-

impurities

-

NCBI accession no.

NM_000074.2

NCBI accession no.

-

NCBI accession no.

-

NCBI accession no.

N/A

UniProt accession no.

P29965

UniProt accession no.

P29965

UniProt accession no.

P29965

UniProt accession no.

P29965

General description

CD40 Ligand (CD40L/TRAP) is a type II membrane protein which, effectuates the helper function of T cells on resting B cells. GF101 is a 16.3 kDa protein containing 149 amino acid residues comprising the receptor binding TNF-like domain of CD40L.
Product Source: Recombinant human

Physical form

Sterile filtered and lyophilized from 10 mM Sodium Phosphate, pH 7.5.

Storage and Stability

The lyophilized protein is stable for a few weeks for at room temperature but is best stored at -20ºC. Reconstitute in water to 0.1-1.0 mg/ml. This solution can be diluted into other aqueous buffers and stored at 2-8ºC for one week or -20ºC for future use. Reconstituted sCD40L/TRAP should be stored at -20ºC in undiluted aliquots for up to 6 months. Freeze/thaw cycles will result in significant loss of activity.

Analysis Note

Specific Activity: GF101 is fully biologically active as determined by its ability to stimulate the proliferation of HEK-BlueTMCD40 reporter cells.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

11 - Combustible Solids

WGK

WGK 1


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