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GF154

Sigma-Aldrich

Wnt-3a, recombinant mouse

The recombinant murine Wnt-3a protein is a monomeric glycoprotein containing 328 amino acid residues.

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eCl@ss:
32160405

biological source

mouse

Quality Level

mol wt

Mw 38.0-41.0 kDa

technique(s)

cell culture | mammalian: suitable

impurities

<0.1 ng/μg endotoxin (1EU/μg)

input

sample type: mouse embryonic stem cell(s)
sample type induced pluripotent stem cell(s)

UniProt accession no.

shipped in

dry ice

Gene Information

mouse ... WNT3A(22416)

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vibrant-m

GF154

Wnt-3a, recombinant mouse

vibrant-m

SRP3296

WNT-7A human

technique(s)

cell culture | mammalian: suitable

technique(s)

-

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

UniProt accession no.

P27467

UniProt accession no.

O00755

UniProt accession no.

P01579, P01580

UniProt accession no.

P61812

mol wt

Mw 38.0-41.0 kDa

mol wt

35.5 kDa

mol wt

-

mol wt

-

Gene Information

mouse ... WNT3A(22416)

Gene Information

human ... WNT7A(7476)

Gene Information

-

Gene Information

human ... TGFB2(7042)

Quality Level

100

Quality Level

-

Quality Level

100

Quality Level

100

General description

Product Source: Mouse L cells expressing full length Wnt3a.
Recombinant murine Wnt-3a is a monomeric glycoprotein containing 328 amino acid residues. When analyzed by SDS-PAGE under non-reducing conditions, the murine Wnt-3a migrates at an apparent molecular weight of approximately 38.0-41.0 kDa.

Wnt-3a signalling has been implicated in the control of differentiation of stem cells. Wnt proteins bind to receptors of the Frizzled family in conjunction with a coreceptor of the low-density lipoprotein receptor-related protein family (LRP-5 or -6), or the Ryk atypical receptor tyrosine kinase. Wnt proteins have been shown to regulate cell-to-cell interactions during embryogenesis, more specifically regulating mesoderm differentiation and osteogenesis. Wnt proteins have also been shown to have putative roles in the regulation of adult stem cells.

Application

Research Category
Stem Cell Research
The recombinant murine Wnt-3a protein is a monomeric glycoprotein containing 328 amino acid residues.

Linkage

Replaces: GF160

Physical form

Lyophilized from a solution containing PBS and 1.0% CHAPS buffer.

Storage and Stability

Maintain the lyophilized material at -20°C up to six months from date of receipt. Resuspend in sterile water to a concentration of 100µg/mL. Reconstituted Wnt3a should be stored in single use aliquots at -80°C for up to three months. Avoid repeated freeze/thaw cycles. During shipment, some amount of product will occasionally dispersed. It’s recommended to gently tape the vial on the hard surface before reconstitution.

Analysis Note

Specific Activity: Wnt3a protein has been shown to be bioactive as determined by TCF/LEF-luciferase reporter. The ED50 for this effect is typically <250 ng/mL. Optimal concentrations should be determined for each application.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

11 - Combustible Solids

wgk_germany

WGK 2


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