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SRP3227

Sigma-Aldrich

NOGGIN from mouse

recombinant, expressed in E. coli, ≥98% (SDS-PAGE), ≥98% (HPLC), suitable for cell culture

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NACRES:
NA.32

biological source

mouse

recombinant

expressed in E. coli

Assay

≥98% (HPLC)
≥98% (SDS-PAGE)

form

lyophilized

potency

1.0-2.0 ng/mL ED50

mol wt

46.4 kDa

packaging

pkg of 20 μg

technique(s)

cell culture | mammalian: suitable

impurities

<0.1 EU/μg endotoxin, tested

color

white to off-white

UniProt accession no.

shipped in

wet ice

storage temp.

−20°C

Gene Information

mouse ... NOG(18121)

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SRP3227

NOGGIN from mouse

Noggin human recombinant, expressed in HEK 293 cells, suitable for cell culture

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N17001

Noggin human

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SRP3196

EGF from mouse

recombinant

expressed in E. coli

recombinant

expressed in NSO cells

recombinant

expressed in HEK 293 cells

recombinant

expressed in E. coli

assay

≥98% (HPLC), ≥98% (SDS-PAGE)

assay

>95% (SDS-PAGE)

assay

≥98% (SDS-PAGE)

assay

≥98% (HPLC), ≥98% (SDS-PAGE)

form

lyophilized

form

lyophilized powder

form

lyophilized powder

form

lyophilized

mol wt

46.4 kDa

mol wt

55-60 kDa by SDS-PAGE (reducing), calculated mol wt 50 kDa

mol wt

23 kDa (The protein migrates as a 25 kDa band on SDS-PAGE due to glycosylation)

mol wt

6.0 kDa

packaging

pkg of 20 μg

packaging

pkg of 50 μg

packaging

-

packaging

pkg of 500 μg

General description

NOGGIN was first identified in the Xenopus embryos in an expression screen for activities that induce dorsal structures. It is a glycoprotein that is released as a homodimer. This protein is expressed during Xenopus gastrula stage. NOGGIN shows major expression in the central nervous system and is also expressed in lung, skin, skeletal muscle, cartilage, and bone. Recombinant murine Noggin is a 46.4 kDa disulfide-linked homodimer consisting of two 206 amino acid polypeptide chains.

Application

NOGGIN from mouse has been used as a supplement in the knockout serum replacement constituting the embryoid body medium containing DMEM/F-12. It has also been used as a supplement in PPC (photoreceptor progenitor cell) intermediate medium.

Biochem/physiol Actions

NOGGIN proteins interact with BMPs (bone morphogenetic protein) and inhibit the activation of BMPRs. In Xenopus gastrula stage, NOGGIN is released by the Spemann organizer, and stimulates neural tissue from dorsal ectoderm by inhibiting ectodermal BMPs. In mouse embryo, this protein is not essential for neural induction, but is crucial for the later development of the neural tube, somite, and cartilage morphogenesis. Double homozygous mutant mice of NOGGIN and CHORDIN show prosencephalon developmental defects. In vitro it functions as a negative regulator of neuronal differentiation of neocortical precursors.
Noggin belongs to a group of diffusible proteins which bind to ligands of the TGF-β family and regulate their activity by inhibiting their access to signaling receptors. Recombinant murine Noggin is a 46.4 kDa disulfide-linked homodimer consisting of two 206 amino acid polypeptide chains.

Sequence

MQHYLHIRPA PSDNLPLVDL IEHPDPIFDP KEKDLNETLL RSLLGGHYDP GFMATSPPED RPGGGGGPAG GAEDLAELDQ LLRQRPSGAM PSEIKGLEFS EGLAQGKKQR LSKKLRRKLQ MWLWSQTFCP VLYAWNDLGS RFWPRYVKVG SCFSKRSCSV PEGMVCKPSK SVHLTVLRWR CQRRGGQRCG WIPIQYPIIS ECKCSC

Physical form

Lyophilized with no additives.

Reconstitution

Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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Conditional inactivation of noggin in the postnatal skeleton causes osteopenia.
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