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MAB3584

Sigma-Aldrich

Anti-Heterochromatin Protein-1 α Antibody, clone 2HP-1H5

ascites fluid, clone 2HP-1H5, Chemicon®

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Synonym(s):
HP1a, Antigen p25, Chromobox Protein Homolog 5
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

antibody form

ascites fluid

antibody product type

primary antibodies

clone

2HP-1H5, monoclonal

species reactivity

mouse, human

manufacturer/tradename

Chemicon®

technique(s)

ELISA: suitable
immunocytochemistry: suitable
immunohistochemistry: suitable

isotype

IgG1κ

suitability

not suitable for Western blot

NCBI accession no.

UniProt accession no.

shipped in

dry ice

target post-translational modification

unmodified

Gene Information

human ... CBX5(23468)

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MAB3446WH0023468M1MABE666
Gene Information

human ... CBX5(23468)

Gene Information

human ... CBX5(23468)

Gene Information

human ... CBX5(23468)

Gene Information

human ... CBX5(23468)

clone

2HP-1H5, monoclonal

clone

2HP-2G9, monoclonal

clone

1E11-3A10, monoclonal

clone

4C12.1, monoclonal

species reactivity

mouse, human

species reactivity

mouse, human

species reactivity

human

species reactivity

mouse, human

antibody form

ascites fluid

antibody form

ascites fluid

antibody form

purified immunoglobulin

antibody form

purified immunoglobulin

UniProt accession no.

P45973

UniProt accession no.

P45973

UniProt accession no.

P45973

UniProt accession no.

P45973

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General description

Chromatin assembly factor-1 (CAF-1) is a multisubunit protein complex that comprises three polypeptide subunits known as p150, p60, and p48. CAF-1 is a nucleosome assembly factor that deposits newly synthesized and acetylated histones H3/H4 into nascent chromatin during DNA replication.

Heterochromatin is characterized as densely coiled chromatin that generally replicates late during S phase, has a low gene density, and contains large blocks of repetitive DNA that is relatively inaccessible to DNA-modifying reagents. In late S phase, p150 directly associates with heterochromatin associated proteins 1 (HP1α, HP1β and HP1γ). As cells prepare for mitosis, CAF-1 p150 and some HP1 progressively dissociate from heterochromatin, coinciding with the phosphorylation of histone H3. The HP1 proteins reassociate with chromatin at the end of mitosis, as histone H3 is dephosphorylated.

Specificity

Reacts with Heterochromatin protein-1 alpha (HP1alpha) between amino acids 67 and 119.

Immunogen

Recombinant mouse HP1a.

Application

Immunocytochemistry: 1:500-1:5,000

Immunohistochemistry: 1:500-1:5,000

ELISA: 1:500-1:5,000

Western blot: not recommended. For Western blot use MAB3446.

Optimal working dilutions must be determined by the end user.
Use Anti-Heterochromatin Protein-1 α Antibody, clone 2HP-1H5 (mouse monoclonal antibody) validated in ELISA, ICC, IHC to detect Heterochromatin Protein-1 α also known as HP1a, Antigen p25, Chromobox Protein Homolog 5.

Physical form

Liquid ascites no preservative.

Analysis Note

Control
Positive Control: HeLa cells, 3T3 cells , F9 cells, mouse embryos

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class

10 - Combustible liquids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable


Certificates of Analysis (COA)

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Putative Epimutagens in Maternal Peripheral and Cord Blood Samples Identified Using Human Induced Pluripotent Stem Cells.
Arai, Y; Hayakawa, K; Arai, D; Ito, R; Iwasaki, Y; Saito, K; Akutsu, K; Takatori et al.
BioMed Research International null
Targeting polycomb to pericentric heterochromatin in embryonic stem cells reveals a role for H2AK119u1 in PRC2 recruitment.
Cooper, S; Dienstbier, M; Hassan, R; Schermelleh, L; Sharif, J; Blackledge, NP; De Marco et al.
Cell Reports null
DNA methylation promotes Aurora-B-driven phosphorylation of histone H3 in chromosomal subdomains.
Monier, K; Mouradian, S; Sullivan, KF
Journal of Cell Science null
Dounia Djeghloul et al.
Stem cell reports, 6(6), 970-984 (2016-06-16)
The capacity of hematopoietic stem cells (HSC) to generate B lymphocytes declines with age, contributing to impaired immune function in the elderly. Here we show that the histone methyltransferase SUV39H1 plays an important role in human B lymphoid differentiation and
Mammalian ChlR1 has a role in heterochromatin organization.
Inoue, A; Hyle, J; Lechner, MS; Lahti, JM
Experimental Cell Research null

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