MilliporeSigma
All Photos(1)

Documents

MAB397A4

Sigma-Aldrich

Anti-GluR2 Antibody, clone 6C4, Alexa Fluor 488 Conjugate

clone 6C4, from mouse, ALEXA FLUOR 488

Sign Into View Organizational & Contract Pricing

Synonym(s):
Glutamate receptor 2, GluR-2, AMPA-selective glutamate receptor 2, GluR-B, GluR-K2, Glutamate receptor ionotropic, AMPA 2
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

conjugate

ALEXA FLUOR 488

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

6C4, monoclonal

species reactivity

mouse, rat

technique(s)

immunocytochemistry: suitable
immunohistochemistry: suitable

isotype

IgG2a

NCBI accession no.

UniProt accession no.

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... GRIA2(2891)

Compare Similar Items

View Full Comparison

Show Differences

1 of 4

This Item
MABN71MAB397MABN103
Anti-GluR2 Antibody, clone L21/32 clone L21/32, from mouse

MABN71

Anti-GluR2 Antibody, clone L21/32

Gene Information

human ... GRIA2(2891)

Gene Information

human ... GRIA2(2891)

Gene Information

dog ... Gria2(482667)
mouse ... Gria2(14800)
rat ... Gria2(29627)
rhesus monkey ... Gria2(574344)

Gene Information

Rattus norvegicus ... Gria2(29627)

antibody form

purified immunoglobulin

antibody form

purified antibody

antibody form

purified immunoglobulin

antibody form

purified antibody

species reactivity

mouse, rat

species reactivity

rat

species reactivity

monkey

species reactivity

rat

conjugate

ALEXA FLUOR 488

conjugate

-

conjugate

-

conjugate

-

clone

6C4, monoclonal

clone

L21/32, monoclonal

clone

6C4, monoclonal

clone

022.19.5, monoclonal

Still not finding the right product?  

Give our Product Selector Tool a try.

General description

Glutamate receptors (GluRs) can be categorized as ionotropic or metabotropic and subcatergorized by their agonist preferences (NMDA, AMPA or Kainic acid). There are four types of AMPA selective GluR subunits (GluR1, GluR2, GluR3 and GluR4). Tetrameric or pentameric combinations of different subunits contributes to the functional diversity of AMPA receptors. In general, AMPA receptors mediate fast synaptic current at most excitatory synapses, with stoichiometry characterized by subtype composition. Although subunit composition of AMPA receptors varies, they must contain at least one edited GluR2 subunit to be calcium impermeable. The critical residue controlling calcium permeability is in the pore loop region. In GluR1, GluR3, and GluR4, this positionis occupied by a Gln residue. In GluR2, it is occupied by an Arg residue. It has been shown experimentally that the presence of Arg in this position blocks CA2+ ion permeability, while a Gln does not. Relative calcium permeability in AMPA receptor channels may be significant in pathological neurotoxic damage and long term changes in nervous system responses.

Specificity

Recognizes the large N-terminal extracellular domain of Glutamate Receptor 2 (GluR2). No cross-reactivity observed with other AMPA/Kainate GluR subunits.

Immunogen

Epitope: Extracellular domain
Recombinant fusion protein TrpE-GluR2

Application

Immunocytochemistry analysis: A 1:100 dilution from a representative lot detected GluR2 in rat E18 primary cortex cells.
Research Category
Neuroscience
Research Sub Category
Developmental Neuroscience
This Anti-GluR2 Antibody, clone 6C4, Alexa Fluor 488 Conjugate is validated for use in IH, IC for the detection of GluR2.

Quality

Evaluated by Immunohistochemistry in adult mouse brain tissue.

Immunohistochemistry Analysis: A 1:100 dilution of this antibody detected GluR2 in adult mouse brain tissue.

Target description

The unconjugated parent antibody (Cat. No. MAB397) has an observed molecular weight at ~102 kDa

Physical form

Protein A purified
Purified mouse monoclonal IgG2a conjugated to Alexa Fluor 488 in PBS with 0.1% sodium azide and 15 mg/mL BSA.

Storage and Stability

Maintain refrigerated at 2-8 °C protected from light in undiluted aliquots for up to 6 months from date of receipt.

Analysis Note

Control
Adult mouse brain tissue

Legal Information

ALEXA FLUOR is a trademark of Life Technologies

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Documents related to the products that you have purchased in the past have been gathered in the Document Library for your convenience.

Visit the Document Library

Difficulty Finding Your Product Or Lot/Batch Number?

Product numbers are combined with Pack Sizes/Quantity when displayed on the website (example: T1503-25G). Please make sure you enter ONLY the product number in the Product Number field (example: T1503).

Example:

T1503
Product Number
-
25G
Pack Size/Quantity

Additional examples:

705578-5MG-PW

PL860-CGA/SHF-1EA

MMYOMAG-74K-13

1000309185

enter as 1.000309185)

Having trouble? Feel free to contact Technical Service for assistance.

Lot and Batch Numbers can be found on a product's label following the words 'Lot' or 'Batch'.

Aldrich Products

  • For a lot number such as TO09019TO, enter it as 09019TO (without the first two letters 'TO').

  • For a lot number with a filling-code such as 05427ES-021, enter it as 05427ES (without the filling-code '-021').

  • For a lot number with a filling-code such as STBB0728K9, enter it as STBB0728 without the filling-code 'K9'.

Not Finding What You Are Looking For?

In some cases, a COA may not be available online. If your search was unable to find the COA you can request one.

Request COA

Boris Brant et al.
Molecular psychiatry (2021-09-19)
Mutations in the IQSEC2 gene are associated with drug-resistant, multifocal infantile and childhood epilepsy; autism; and severe intellectual disability (ID). We used induced pluripotent stem cell (iPSC) technology to obtain hippocampal neurons to investigate the neuropathology of IQSEC2-mediated disease. The
Eli J Rogers et al.
Frontiers in molecular neuroscience, 12, 43-43 (2019-03-08)
We have recently described an A350V mutation in IQSEC2 associated with intellectual disability, autism and epilepsy. We sought to understand the molecular pathophysiology of this mutation with the goal of developing targets for drug intervention. We demonstrate here that the
G Aleph Prieto et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience, 37(5), 1197-1212 (2016-12-18)
Long-term potentiation (LTP) is an activity-dependent and persistent increase in synaptic transmission. Currently available techniques to measure LTP are time-intensive and require highly specialized expertise and equipment, and thus are not well suited for screening of multiple candidate treatments, even

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service