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MABE343-AF647

Sigma-Aldrich

Anti-Puromycin, clone 12D10, Alexa Fluor 647 Conjugate Antibody

clone 12D10, 0.5 mg/mL, from mouse

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eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

conjugate

ALEXA FLUOR 647

antibody form

purified antibody

antibody product type

primary antibodies

clone

12D10, monoclonal

species reactivity

human

concentration

0.5 mg/mL

technique(s)

immunocytochemistry: suitable

shipped in

wet ice

target post-translational modification

unmodified

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This Item
MABE343-AF488MABE341-AF488MABE343
conjugate

ALEXA FLUOR 647

conjugate

ALEXA FLUOR 488

conjugate

ALEXA FLUOR 488

conjugate

-

antibody form

purified antibody

antibody form

purified antibody

antibody form

purified antibody

antibody form

purified immunoglobulin

clone

12D10, monoclonal

clone

12D10, monoclonal

clone

17H1, monoclonal

clone

12D10, monoclonal

species reactivity

human

species reactivity

human

species reactivity

human

species reactivity

human

concentration

0.5 mg/mL

concentration

0.5 mg/mL

concentration

0.5 mg/mL

concentration

-

General description

Puromycin is an aminonucleoside antibiotic, derived from the Streptomyces alboniger bacterium, that functions as a protein synthesis inhibitor that blocks translation through premature chain termination in the ribosome. Monoclonal antibodies to puromycin may be used with standard immunochemical methods to directly monitor translation, a method known as surface sensing of translation (SUnSET). Part of the molecule resembles the 3′ end of the aminoacylated tRNA, making it useful for protein translation analysis. Puromycin induces DNA fragmentation in thymocytes and in human HL-60 leukemia cells.
This Anti-Puromycin, clone 12D10 is validated for use in Immunocytochemistry for the detection of Puromycin.

Specificity

Predicted to react with cells from all species when pre-incubated with puromycin.

Quality

Evaluated by Immunocytochemistry Analysis untreated and puromycin treated HeLa cells.

Immunocytochemistry Analysis: A 1:2,500 dilution of this antibody detected puromycin-incorporated neosynthesized proteins in puromycin treated HeLa cells.

Alexa Fluor is a registered trademark of Life Technologies.

Target description

Refer to Cat. No. MABE343 for observed molecular weight information.

Legal Information

ALEXA FLUOR is a trademark of Life Technologies

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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