MilliporeSigma
All Photos(1)

Documents

MABE341-AF488

Sigma-Aldrich

Anti-Puromycin, clone 17H1, Alexa Fluor 488 Conjugate Antibody

clone 17H1, 0.5 mg/mL, from rat

Sign Into View Organizational & Contract Pricing

Select a Size

eCl@ss:
32160702
NACRES:
NA.41

biological source

rat

Quality Level

conjugate

ALEXA FLUOR 488

antibody form

purified antibody

antibody product type

primary antibodies

clone

17H1, monoclonal

species reactivity

human

concentration

0.5 mg/mL

technique(s)

immunocytochemistry: suitable

shipped in

wet ice

target post-translational modification

unmodified

Compare Similar Items

View Full Comparison

Show Differences

1 of 4

This Item
MABE343-AF488MABE343-AF647MABE341
conjugate

ALEXA FLUOR 488

conjugate

ALEXA FLUOR 488

conjugate

ALEXA FLUOR 647

conjugate

-

antibody form

purified antibody

antibody form

purified antibody

antibody form

purified antibody

antibody form

purified antibody

clone

17H1, monoclonal

clone

12D10, monoclonal

clone

12D10, monoclonal

clone

17H1, monoclonal

species reactivity

human

species reactivity

human

species reactivity

human

species reactivity

human

concentration

0.5 mg/mL

concentration

0.5 mg/mL

concentration

0.5 mg/mL

concentration

-

General description

Puromycin is an aminonucleoside antibiotic, derived from the Streptomyces alboniger bacterium, that functions as a protein synthesis inhibitor that blocks translation through premature chain termination in the ribosome. Monoclonal antibodies to puromycin may be used with standard immunochemical methods to directly monitor translation, a method known as surface sensing of translation (SUnSET). Part of the molecule resembles the 3′ end of the aminoacylated tRNA, making it useful for protein translation analysis. Puromycin induces DNA fragmentation in thymocytes and in human HL-60 leukemia cells.
This product is a conjugated version of Cat. No. MABE341.

Specificity

Demonstrated to react with Human test sample, preincubated with Puromycin. Predicted to react with all species when test sample is incubated with Puromycin.
This antibody detects Puromycin-incorporated neosynthesized proteins.

Immunogen

Puromycin-incorporated neosynthesized proteins at multiple molecular weights

Application

Research Category
Epigenetics & Nuclear Function
Research Sub Category
Cell Cycle, DNA Replication & Repair
This Anti-Puromycin, clone 17H1 is validated for use in Immunocytochemistry for the detection of Puromycin.

Quality

Evaluated by Immunocytochemistry in untreated and Puromycin treated HeLa cells.

Immunocytochemistry Analysis: A 1:500 dilution of this antibody detected Puromycin-incorporated neosynthesized proteins in HeLa cells treated with Puromycin only.

Alexa Fluor is a registered trademark of Life Technologies.

Target description

Refer to Cat. No. MABE341 for observed molecular weight information.

Physical form

Protein G Purified
Purified rat monoclonal conjugate in buffer containing PBS and 15 mg/ml BSA with 0.1% Sodium Azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Legal Information

ALEXA FLUOR is a trademark of Life Technologies

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Documents related to the products that you have purchased in the past have been gathered in the Document Library for your convenience.

Visit the Document Library

Difficulty Finding Your Product Or Lot/Batch Number?

Product numbers are combined with Pack Sizes/Quantity when displayed on the website (example: T1503-25G). Please make sure you enter ONLY the product number in the Product Number field (example: T1503).

Example:

T1503
Product Number
-
25G
Pack Size/Quantity

Additional examples:

705578-5MG-PW

PL860-CGA/SHF-1EA

MMYOMAG-74K-13

1000309185

enter as 1.000309185)

Having trouble? Feel free to contact Technical Service for assistance.

Lot and Batch Numbers can be found on a product's label following the words 'Lot' or 'Batch'.

Aldrich Products

  • For a lot number such as TO09019TO, enter it as 09019TO (without the first two letters 'TO').

  • For a lot number with a filling-code such as 05427ES-021, enter it as 05427ES (without the filling-code '-021').

  • For a lot number with a filling-code such as STBB0728K9, enter it as STBB0728 without the filling-code 'K9'.

Not Finding What You Are Looking For?

In some cases, a COA may not be available online. If your search was unable to find the COA you can request one.

Request COA

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service