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06-984

Sigma-Aldrich

Anti-Mn-SOD Antibody

Upstate®, from rabbit

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Synonym(s):
Mn superoxide dismutase, indophenoloxidase B, manganese superoxide dismutase, manganese-containing superoxide dismutase, mangano-superoxide dismutase, superoxide dismutase 2, mitochondrial
eCl@ss:
32160702
NACRES:
NA.41

biological source

rabbit

Quality Level

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

polyclonal

species reactivity

mouse, bovine, human, rat

should not react with

canine

packaging

antibody small pack of 25 μg

manufacturer/tradename

Upstate®

technique(s)

immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
western blot: suitable

isotype

IgG

NCBI accession no.

UniProt accession no.

shipped in

ambient

target post-translational modification

unmodified

Gene Information

human ... SOD2(6648)

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This Item
S506907-403-IS5569
Anti-Mn-SOD Antibody Upstate®, from rabbit

06-984

Anti-Mn-SOD Antibody

Anti-Cu/Zn-SOD Antibody from rabbit, purified by affinity chromatography

07-403-I

Anti-Cu/Zn-SOD Antibody

clone

polyclonal

clone

polyclonal

clone

polyclonal

clone

polyclonal

species reactivity

mouse, bovine, human, rat

species reactivity

rat, human

species reactivity

human, mouse, rat

species reactivity

rat, human

biological source

rabbit

biological source

rabbit

biological source

rabbit

biological source

rabbit

Gene Information

human ... SOD2(6648)

Gene Information

human ... SOD2(6648)
rat ... Sod2(24787)

Gene Information

human ... SOD1(6647)

Gene Information

human ... SOD2(6648)
mouse ... Sod2(20656)
rat ... Sod2(24787)

shipped in

ambient

shipped in

dry ice

shipped in

wet ice

shipped in

dry ice

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General description

Superoxide dismutase (SOD) is an antioxidant enzyme involved in the defense system against reactive oxygen species (ROS). SOD catalyzes the dismutation reaction of superoxide radical anion (O2-) to hydrogen peroxide, which is then catalyzed to innocuous O2 and H2O by glutathione peroxidase and catalase. Several classes of SOD have been identified. These include intracellular copper, zinc SOD (Cu, Zn SOD/SOD1), mitochondrial manganese SOD (Mn SOD/SOD2) and extracellular Cu, Zn SOD (EC SOD/SOD3). The manganese containing 80 kDa tetrameric enzyme SOD2 is located in the mitochondrial matrix in close proximity to a primary endogenous source of superoxide, the mitochondrial respiratory chain.

Specificity

Recognizes MnSOD, Mr 24 kDa.

Immunogen

Recombinant human MnSOD (manganous superoxide dismutase).

Application

Anti-Mn-SOD Antibody is an antibody against Mn-SOD for use in IH, IH(P) & WB.
Immunohistochemistry (Paraffin):
10 μg/mL of a previous lot detected MnSOD in paraffin embedded rat brain tissue sections.

Quality

Evaluated by western blot on RIPA lysates of MCF-7, A431 or rat L6 RIPA cells.

Western Blot Analysis:
0.5-2 μg/mL of this antibody detected MnSOD in RIPA lysates of rat L6 RIPA cells. 1-4 μg/mL of a previous lot detected MnSOD in RIPA lysates of human MCF-7, A431 and rat L6 RIPA cells.

Target description

~24 kDa

Physical form

Format: Purified
Purified rabbit polyclonal in buffer containing 70% storage buffer 0.1 M Tris-glycine, pH 7.4, 0.15 M NaCl, 0.05% sodium azide and 30% glycerol.

Analysis Note

Control
L6 cell lysate, rat brain tissue extract.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

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Manganese superoxide dismutase expression in human cancer cells: a possible role of mRNA processing.
St Clair, D K and Oberley, L W
Free Radical Research Communications, 12-13 Pt 2, 771-778 (1991)
Metabolic utilization of exogenous pyruvate by mutant p53 (R175H) human melanoma cells promotes survival under glucose depletion.
Valery A Chavez-Perez,Mary Strasberg-Rieber,Manuel Rieber
Cancer Biology & Therapy null
Low concentrations of methamphetamine can protect dopaminergic cells against a larger oxidative stress injury: mechanistic study.
El Ayadi, A; Zigmond, MJ
Testing null
Jeffrey S Murley et al.
Free radical biology & medicine, 40(6), 1004-1016 (2006-03-17)
The free radical scavenger WR1065 (SH) is the active thiol form of the clinically approved cytoprotector amifostine. At doses of 40 microM and 4 mM it can activate the redox-sensitive nuclear transcription factor kappaB (NFkappaB) and elevate the expression of
Xiaoke Yin et al.
Proteomics, 6(24), 6437-6446 (2006-12-14)
Embryonic stem (ES) cells can differentiate into vascular smooth muscle cells (SMCs), but differences in protein composition, function and behaviour between stem cell-derived and mature SMCs remain to be characterized. Using differential in gel electrophoresis (DIGE) and MS, we identified

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