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MSHMAG-21K

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MILLIPLEX® Multi-Species Hormone Magnetic Bead Panel

The Multi-Species Hormone Panel, using the Luminex xMAP technology, enables the simultaneous analysis of 5 steroid/thyroid biomarkers in human serum, plasma and cell culture samples.

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Synonym(s):
luminex multiple species thyroid reproductive stress endocrine metabolic hormones multiplex assay, millipore multiple species metabolism hormones multiplex kit, multi species hormone immunoassay panel
eCl@ss:
32161000

Quality Level

species reactivity

human, canine

manufacturer/tradename

Milliplex®

assay range

accuracy: 105%
(Canine Samples: T3)

accuracy: 80%
(Canine Samples: Estradiol)

accuracy: 85%
(Human Samples: Progesterone)

accuracy: 87%
(Canine Samples: T4)

sensitivity: 0.01 ng/mL
(MinDC+2SD; Estradiol)

sensitivity: 0.12 ng/mL
(MinDC+2SD; T3)

sensitivity: 0.25 ng/mL
(MinDC+2SD; Progesterone)

sensitivity: 0.33 ng/mL
(MinDC+2SD; Cortisol)

sensitivity: 0.51 ng/mL
(MinDC+2SD; T4)

standard curve range: 0.014-10 ng/mL
(Estradiol)

standard curve range: 0.07-50 ng/mL
(T3)

standard curve range: 0.14-100 ng/mL
(Progesterone)

standard curve range: 0.27-200 ng/mL
(Cortisol)

standard curve range: 0.34-250 ng/mL
(T4)

inter-assay cv: <10%
intra-assay cv: <10%
(Cortisol)

inter-assay cv: <15%
intra-assay cv: <10%
(Estradiol)

inter-assay cv: <15%
intra-assay cv: <10%
(Progesterone)

inter-assay cv: <15%
intra-assay cv: <10%
(T3)

inter-assay cv: <15%
intra-assay cv: <10%
(T4)

technique(s)

multiplexing: suitable

detection method

fluorometric (Luminex xMAP)

shipped in

ambient

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CGTMAG-98KMADKMAG-71KHCYTOMAG-60K
manufacturer/tradename

Milliplex®

manufacturer/tradename

Milliplex®

manufacturer/tradename

Milliplex®

manufacturer/tradename

Milliplex®

assay range

accuracy: 105%
(Canine Samples: T3), accuracy: 80%
(Canine Samples: Estradiol), accuracy: 85%
(Human Samples: Progesterone), accuracy: 87%
(Canine Samples: T4), sensitivity: 0.01 ng/mL
(MinDC+2SD; Estradiol), sensitivity: 0.12 ng/mL
(MinDC+2SD; T3), sensitivity: 0.25 ng/mL
(MinDC+2SD; Progesterone), sensitivity: 0.33 ng/mL
(MinDC+2SD; Cortisol), sensitivity: 0.51 ng/mL
(MinDC+2SD; T4), standard curve range: 0.014-10 ng/mL
(Estradiol), standard curve range: 0.07-50 ng/mL
(T3), standard curve range: 0.14-100 ng/mL
(Progesterone), standard curve range: 0.27-200 ng/mL
(Cortisol), standard curve range: 0.34-250 ng/mL
(T4), inter-assay cv: <10%
intra-assay cv: <10%
(Cortisol), inter-assay cv: <15%
intra-assay cv: <10%
(Estradiol), inter-assay cv: <15%
intra-assay cv: <10%
(Progesterone), inter-assay cv: <15%
intra-assay cv: <10%
(T3), inter-assay cv: <15%
intra-assay cv: <10%
(T4)

assay range

accuracy: 100%
(GLP-1(Active)), accuracy: 101%
(Glucagon), accuracy: 103%
(Amylin(Total)), accuracy: 98%
(PYY(total)), accuracy: 99%
(GIP), accuracy: 99%
(Ghrelin(Active)), accuracy: 99%
(Leptin), accuracy: 99%
(PP), sensitivity: 0.6 pg/mL
(Minimum Detectable Concentration (MinDC +2SD); GIP (total)), sensitivity: 13.1 pg/mL
(Minimum Detectable Concentration (MinDC +2SD); PYY (Total)), sensitivity: 3.3 pg/mL
(Minimum Detectable Concentration (MinDC +2SD); Ghrelin (active)), sensitivity: 3.6 pg/mL
(Minimum Detectable Concentration (MinDC +2SD); PP), sensitivity: 43.6 pg/mL
(Minimum Detectable Concentration (MinDC +2SD); Glucagon), sensitivity: 5.9 pg/mL
(Minimum Detectable Concentration (MinDC +2SD); Amylin (total)), sensitivity: 79.3 pg/mL
(Minimum Detectable Concentration (MinDC +2SD); Insulin), sensitivity: 9.0 pg/mL
(Minimum Detectable Concentration (MinDC +2SD); GLP-1 (active)), sensitivity: 92.1 pg/mL
(Minimum Detectable Concentration (MinDC +2SD); Leptin), standard curve range: 0.5-2,000 pg/mL
(GIP), standard curve range: 0.7-3,000 pg/mL
(Ghrelin (Active)), standard curve range: 2.4-10,000 pg/mL
(GLP-1 (Active), PP, PYY (Total)), standard curve range: 24.4-100,000 pg/mL
(Insulin, Leptin), standard curve range: 4.9-20,000 pg/mL
(Glucagon)

assay range

accuracy: 77-92%, standard curve range: 12.2-50,000 pg/mL

assay range

accuracy: 87-107%, standard curve range: 3.2-10,000 pg/mL
(sCD40L, EGF, Eotaxin/CCL11, FGF-2, Flt-3 ligand, Fractalkine, G-CSF, GM-CSF, GRO, IFN-α2, IFN-γ, IL-1α, IL-1β, IL-1ra, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-10, IL-12 (p40), IL-12 (p70), IL-13, IL-15, IL-17A, IP-10, MCP-1, MCP-3, MDC (CCL22), MIP-1α, MIP-1β, PDGF-AB/BB, RANTES, TGF-α, TNF-α, TNF-β, VEGF), inter-assay cv: 5-19%
intra-assay cv: 2-13%

technique(s)

multiplexing: suitable

technique(s)

multiplexing: suitable

technique(s)

multiplexing: suitable

technique(s)

multiplexing: suitable

detection method

fluorometric (Luminex xMAP)

detection method

fluorometric (Luminex xMAP)

detection method

fluorometric (Luminex xMAP)

detection method

fluorometric (Luminex xMAP)

shipped in

ambient

shipped in

wet ice

shipped in

wet ice

shipped in

wet ice

General description

Steroid and thyroid hormones are small molecule endocrine hormones secreted from adrenal glands (cortisol), ovaries (estradiol and progesterone), and the thyroid (T3 and T4). These hormones play key roles in the regulation of energy balance, mental and physical development, sex organ development, reproduction, and pregnancy. These hormones play a role in the patho-physiology of many diseases such as thyroid-related diseases (Grave’s disease and hyper/hypothyroidism), steroid-related diseases (Cushing’s syndrome) and stress related diseases. Endocrine disrupting chemincals (EDC) can impact normal functioning of steroid and thyroid hormones.

The MILLIPLEX® Multi-Species Hormone Panel is a 5-plex kit to be used for the simultaneous quantification of any or all of the following analytes in serum, plasma and cell/tissue culture samples: Cortisol, Estradiol, Progesterone, T3 and T4. This multi-species kit may be used to detect these analytes in human, dog, rat, mouse, cynomolgous monkey, rhesus monkey, horse, rabbit, cat, guinea pig, pig and hamster. The kit has been verified with human and canine samples. The amount of cross-reactivity to other species has been confirmed, but not tested to the same level of verification as was used for human and canine. All analytes in this kit are run in a competitive assay format. This kit uses a 96-well format, contains a lyophilized standard cocktail, two internal assay quality controls and can measure up to 38 samples in duplicate.

The Luminex® xMAP® platform uses a magnetic bead immunoassay format for ideal speed and sensitivity to quantitate multiple analytes simultaneously, dramatically improving productivity while conserving valuable sample volume.

Panel Type: Endocrine

Specificity

Approximately 10% cross-reactivity to Cortisol is observed in the Progesterone assay and approximately 5% cross-reactivity to T3 is observed in the T4 assay. No other significant cross-reactivity is observed within the panel or to other similar analytes.

This multi-species kit may be used to detect these 5 analytes in human, dog, rat, mouse, cynomolgous monkey, rhesus monkey, horse, rabbit, cat, guinea pig, pig and hamster. However, the kit was fully validated with human and canine samples only and the amount of cross-reactivity to other species tested has not been determined.
Cross Reactivty
Approximately 10% cross-reactivity to Cortisol is observed in the Progesterone assay and approximately 5% cross-reactivity to T3 is observed in the T4 assay. No other significant cross-reactivity is observed within the panel or to other similar analytes.

Application

  • Analytes: Cortisol, Estradiol, Progesterone, T3, T4
  • NOTE: This multi-species kit may be used to detect these analytes in human, dog, rat, mouse, cynomolgus monkey, rhesus monkey, horse, rabbit, cat, guinea pig, pig and hamster. However, the kit was validated with human and canine samples only and the amount of cross-reactivity to other species tested, has not been determined.
  • NOTE: This assay requires an extraction step for serum and plasma samples (instructions are provided in the kit protocol).
  • NOTE: This is a competitive immunoassay
  • Recommended Sample Type: Serum, plasma, or tissue/cell culture supernatants or lysates from multiple species
  • Recommended Sample Dilution: 25 μL per well of undiluted extracted serum or plasma. Tissue/cell culture samples may be run neat or diluted in an appropriate control media.
  • Assay Run Time: Overnight (16-20 hours) at 2-8°C.
  • Research Category: Metabolism
  • Research Category: Endocrine

Features and Benefits

Design your multiplex kit by choosing available analytes within this panel.

Packaging

96 well plate
Everything you need in a single kit.

Linkage

Replaces: STTHMAG-21K

Storage and Stability

Recommended storage for kit components is 2 - 8°C.

Other Notes

Please contact Technical Service for linearity of dilution.

Legal Information

Luminex is a registered trademark of Luminex Corp
MILLIPLEX is a registered trademark of Merck KGaA, Darmstadt, Germany
xMAP is a registered trademark of Luminex Corp

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Signal Word

Danger

Hazard Classifications

Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 2 - Eye Dam. 1 - Skin Sens. 1 - STOT RE 2

Target Organs

Respiratory Tract

Storage Class Code

10 - Combustible liquids

WGK

WGK 3


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Gabriela Leite et al.
Menopause (New York, N.Y.), 29(3), 264-275 (2022-02-26)
Hormone therapy (HT) is used to treat menopause-related conditions and symptoms. The small intestine plays key roles in metabolic and endocrine function, but the effects of HT on the small intestinal microbiome are unknown. Here, we characterize duodenal microbiome differences
Christian K Garcia et al.
PloS one, 17(10), e0275715-e0275715 (2022-10-14)
The capability of male mice to exercise in hot environments without succumbing to exertional heat stroke (EHS) is markedly blunted compared to females. Epidemiological evidence in humans and other mammals also suggests some degree of greater vulnerability to heat stroke
Yepei Fu et al.
Experimental and therapeutic medicine, 20(6), 244-244 (2020-11-13)
Kidney-yang deficiency syndrome (KYDS) infected with the influenza virus is a suitable model to imitate a population at high-risk to influenza infection with a high rate of morbidity and mortality. However, the specific molecular mechanisms underlying this disease remain unclear.

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