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S5069

Sigma-Aldrich

Anti-Superoxide Dismutase (MnSOD) (DD-17) antibody produced in rabbit

enhanced validation

affinity isolated antibody, buffered aqueous solution

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Synonym(s):
Anti-IPO-B, Anti-Indophenoloxidase B, Anti-MNSOD, Anti-Manganese Dependent SOD, Anti-Manganese Superoxide Dismutase 2, Anti-Mitochodrial SOD, Anti-Mn-SOD, Anti-SOD2, Anti-Superoxide Dismutase 2
MDL number:
NACRES:
NA.41

biological source

rabbit

Quality Level

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

mol wt

antigen ~24 kDa

species reactivity

rat, human

enhanced validation

independent
Learn more about Antibody Enhanced Validation

technique(s)

immunohistochemistry (formalin-fixed, paraffin-embedded sections): 10-20 μg/mL using biotin/ExtraAvidin®-peroxidase staining of human heart sections
indirect immunofluorescence: 10-20 μg/mL using methanol-acetone-fixed rat NRK cells
western blot: 1-2 μg/mL using human HeLa mitochondria extract and a chemiluminescent detection reagent
western blot: 2-4 μg/mL using whole extract of rat brain and a chemiluminescent detection reagent

UniProt accession no.

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... SOD2(6648)
rat ... Sod2(24787)

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This Item
S1450SAB2102261SAB4200807
biological source

rabbit

biological source

rabbit

biological source

rabbit

biological source

mouse

antibody form

affinity isolated antibody

antibody form

affinity isolated antibody

antibody form

affinity isolated antibody

antibody form

purified from hybridoma cell culture

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

technique(s)

immunohistochemistry (formalin-fixed, paraffin-embedded sections): 10-20 μg/mL using biotin/ExtraAvidin®-peroxidase staining of human heart sections, western blot: 1-2 μg/mL using human HeLa mitochondria extract and a chemiluminescent detection reagent, indirect immunofluorescence: 10-20 μg/mL using methanol-acetone-fixed rat NRK cells, western blot: 2-4 μg/mL using whole extract of rat brain and a chemiluminescent detection reagent

technique(s)

direct immunofluorescence: 2-5 μg/mL using human HeLa, rat NRK and mouse 3T3 cells.

technique(s)

western blot: suitable

technique(s)

indirect ELISA: 0.3-0.6 μg/mL using 5μg/ml Superoxide Dismutase from human erythrocytes for coating

clone

polyclonal

clone

polyclonal

clone

polyclonal

clone

SD-G6, monoclonal

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General description

Superoxide Dismutase (SOD) is a metalloprotein enzyme. It is widely distributed in the animal and plant kingdoms. The three common mammalian endogenous isozymes are the homodimeric copper-zinc SOD (Cu,ZnSOD, SOD1) found primarily in the cytosol, the homotetrameric glycosylated copper-zinc SOD, extracellular SOD (EC-SOD, SOD3), and the homotetrameric mitochondrial manganese SOD (MnSOD, SOD2). All three are nuclear-encoded. MnSOD is composed of four subunits, each containing one Mn2+.

Immunogen

synthetic peptide corresponding to amino acid residues 183-199 of human superoxide dismutase (MnSOD) with N-terminal added cysteine, conjugated to KLH. The corresponding sequence is conserved in many eukaryotes.

Application

The antibody may be detected by various immunochemical techniques including immunoblotting, co-immunoprecipitation assay (co-IP), immunoprecipitation, indirect immunofluorescence and immunohistochemistry.

Biochem/physiol Actions

Superoxide Dismutase (SOD) catalyzes the dismutation of the cytotoxic superoxide radical (O2 -) to molecular oxygen and hydrogen peroxide. Manganese SOD (MnSOD) has been proposed to function as a tumor suppressor by modulating the activity of redox-sensitive transcription factors and specific signal mediators. MnSOD is involved in detoxification.

Physical form

Solution in phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide.

Legal Information

ExtrAvidin is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

10 - Combustible liquids

wgk_germany

nwg

flash_point_f

Not applicable

flash_point_c

Not applicable


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Wen-Su Wei et al.
Redox biology, 48, 102201-102201 (2021-12-06)
Reactive oxygen species (ROS) which are continuously generated mainly by mitochondria, have been proved to play an important role in the stress signaling of cancer cells. Moreover, pentatricopeptide repeat (PPR) proteins have been suggested to take part in mitochondrial metabolism.
Yuanhui Lai et al.
International journal of molecular medicine, 37(2), 369-377 (2015-12-29)
The sensitization of breast cancer stem cells (BrCSCs) to the inhibitive effects of radiotherapy through adjuvant therapy which targets oncogenic pathways represents a prospective strategy for improving the effect of radiation in patients with triple-negative breast cancer (TNBC). Mammalian target
Inhibition of mTOR sensitizes breast cancer stem cells to radiation-induced repression of self-renewal through the regulation of MnSOD and Akt
Lai Y, et al.
International Journal of Molecular Medicine, 37(2), 369-377 (2016)
Tetramerization reinforces the dimer interface of MnSOD
Sheng Y, et al.
PLoS ONE, 8(5), e62446-e62446 (2013)
SOD2 deregulation enhances migration, invasion and has poor prognosis in salivary adenoid cystic carcinoma
Chang B, et al.
Scientific Reports, 6 (2016)

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