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MABE664

Sigma-Aldrich

Anti-CBX1 (HP1 beta) Antibody, clone 5E9.1

clone 5E9.1, from mouse

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Synonym(s):
Chromobox protein homolog 1, Heterochromatin protein 1 homolog beta, HP1 beta, Heterochromatin protein p25, M31, Modifier 1 protein
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

5E9.1, monoclonal

species reactivity

human

packaging

antibody small pack of 25 μg

technique(s)

western blot: suitable

isotype

IgG1κ

NCBI accession no.

UniProt accession no.

shipped in

ambient

target post-translational modification

unmodified

Gene Information

human ... CBX1(10951)

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Anti-HP1γ Antibody, clone 14D3.1 clone 14D3.1, from mouse

MABE656

Anti-HP1γ Antibody, clone 14D3.1

clone

5E9.1, monoclonal

clone

polyclonal

clone

4C12.1, monoclonal

clone

14D3.1, monoclonal

biological source

mouse

biological source

rabbit

biological source

mouse

biological source

mouse

antibody form

purified immunoglobulin

antibody form

affinity isolated antibody

antibody form

purified immunoglobulin

antibody form

purified antibody

species reactivity

human

species reactivity

mouse, human

species reactivity

mouse, human

species reactivity

human

technique(s)

western blot: suitable

technique(s)

immunocytochemistry: suitable, western blot: suitable

technique(s)

immunohistochemistry: suitable (paraffin), western blot: suitable

technique(s)

immunocytochemistry: suitable, western blot: suitable

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General description

Chromobox protein homolog 1 (UniProt: P83916; also known as HP1Hsbeta, heterochromatin protein 1 homolog beta, heterochromatin protein p25, M31, Modifier 1 protein, p25 beta) is encoded by the CBX1 (also known as CBX) gene (Gene ID:10951) in human. Chromobox protein homolog 1 is a homodimeric nuclear protein that is a component of heterochromatin. It is expressed in all adult and embryonic tissues. It is essential for formation of and gene repression in heterochromatin. It recognizes and binds histone H3 tails methylated at Lys-9, leading to epigenetic repression. Also interacts with lamin B receptor (LBR) to contribute to the association of the heterochromatin with the inner nuclear membrane. The human HP1 homolog CBX1 preferentially recognize H3K9me3 (Peptide binding specificity Kd = 5 µM). However, upon substitution of Threonine 6 to Alanine reduces its affinity significantly (Kd = 45 mM). (Ref.: Kaustov, L., et al. (2011). J. Biol. Chem. 286(1): 521-529).

Specificity

Clone 5E9.1 specifically detects human Chromobox protein homolog 1 (CBX1). It targets an epitope with in 60 amino acids from the internal region.

Immunogen

GST/His-tagged recombinant protein fragment corresponding to 60 amino acids from the internal region of human Chromobox protein homolog 1 (CBX1).

Application

Anti-CBX1 (HP1 beta) antibody, clone 5E9.1, Cat. No. MABE664, is a highly specific mouse monoclonal antibody that targets CBX1 and has been tested in Western Blotting.
Research Category
Epigenetics & Nuclear Function

Quality

Evaluated by Western Blotting in HeLa cell lysate.

Western Blotting Analysis: 1 µg/mL of this antibody detected CBX1 (HP1 beta) in 10 µg of HeLa cell lysate.

Target description

~23 kDa observed; 21.42 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

Physical form

Format: Purified
Protein G purified
Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Other Notes

Concentration: Please refer to lot specific datasheet.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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