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06-543

Sigma-Aldrich

Anti-FAK Antibody

Upstate®, from rabbit

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Synonym(s):
FADK 1, PTK2 protein tyrosine kinase 2, Protein-tyrosine kinase 2, focal adhesion kinase 1
eCl@ss:
32160702
NACRES:
NA.41

biological source

rabbit

Quality Level

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

polyclonal

species reactivity

human, hamster, mouse, rat

should not react with

avian

manufacturer/tradename

Upstate®

technique(s)

immunocytochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable

isotype

IgG

NCBI accession no.

UniProt accession no.

shipped in

dry ice

target post-translational modification

unmodified

Gene Information

human ... PTK2(5747)

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This Item
05-113905-1140ABT135
Anti-FAK Antibody Upstate®, from rabbit

06-543

Anti-FAK Antibody

Anti-phospho-FAK (Tyr397) Antibody from rabbit, purified by affinity chromatography

ABT135

Anti-phospho-FAK (Tyr397) Antibody

clone

polyclonal

clone

BLAb2H7, monoclonal

clone

18, monoclonal

clone

polyclonal

antibody form

purified immunoglobulin

antibody form

purified immunoglobulin

antibody form

purified immunoglobulin

antibody form

affinity isolated antibody

species reactivity

human, hamster, mouse, rat

species reactivity

human, mouse, rat, chicken

species reactivity

mouse, human

species reactivity

rat

Gene Information

human ... PTK2(5747)

Gene Information

chicken ... Ptk2(396416)
human ... PTK2(5747)
mouse ... Ptk2(14083)
rat ... Ptk2(25614)

Gene Information

human ... PTK2(5747)
mouse ... Ptk2(14083)

Gene Information

human ... PTK2(5747)

technique(s)

immunocytochemistry: suitable, western blot: suitable, immunoprecipitation (IP): suitable

technique(s)

immunoprecipitation (IP): suitable, western blot: suitable

technique(s)

western blot: suitable

technique(s)

cell based assay: suitable, western blot: suitable

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General description

Focal adhesion kinase (FAK) is a non receptor protein tyrosine kinase discovered as a substrate for Src and as a key element of integrin signaling. FAK plays a central role in cell spreading, differentiation, migration, cell death and acceleration of the G1 to S phase transition of the cell cycle. FAK regulation includes phosphorylation at multiple tyrosine and serine residues. Phosphorylation of tyrosine generally is associated with positive regulation and growth promotion, however, dephosphorylation at these sites occurs as cells enter mitosis (M-Phase of the cell cycle). In contrast, serine phosphorylation either remains high or is increased as cells enter mitosis and may play a role in focal adhesion disassembly. FAK and it′s phosphorylation states have been implicated in cancer metastasis and tumor cell survival and adhesion-independent growth. Additionally, recent evidence indicates that elevation of FAK activity in human carcinoma cells is associated with increased invasive potential. A central role in tumor formation and progression suggests that FAK is an attractive target for therapeutic intervention.

Immunogen

pGEX-derived fusion protein containing residues 748-1053 of human FAK

Application

Immunocytochemistry: 10 μg/mL of a previous lot showed positive immunostaining for Fak in Ref 52 cells fixed with 3.7% formaldehyde.

Immunoprecipitation: 4 μg of a previous lot immunoprecipitated FAK from 500 μg of murine 3T3/A31 RIPA lysate.
Research Category
Signaling
Research Sub Category
Cytoskeletal Signaling
This Anti-FAK Antibody is validated for use in WB, IP, IC for the detection of FAK.

Quality

Evaluated by western blot on RIPA lysates from murine 3T3/A31 or human A431 RIPA cell lysate.

Western Blot Analysis: 0.5-2 μg/mL of this antibody detected FAK in RIPA lysates from murine 3T3/A31 and previously in human A431 RIPA cell lysate.

Target description

125 kDa

Linkage

Replaces: 04-591

Physical form

Format: Purified
Protein A purified
Purified rabbit polyclonal IgG in buffer containing storage buffer (0.1 M Tris-glycine, pH 7.4, 0.15 M NaCl, 0.05% sodium azide). Store at -20°C.

Storage and Stability

Stable for 1 year at from date of receipt.

Analysis Note

Control
Positive Antigen Control: Catalog #12-305, 3T3/A31 lysate. Add 2.5 μL of 2-mercapto-ethanol/100 μL of lysate and boil for 5 minutes to reduce the preparation. Load 20 μg of reduced lysate per lane for minigels.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable


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Focal adhesion kinase-dependent regulation of adhesive forces involves vinculin recruitment to focal adhesions.
David W Dumbauld,Kristin E Michael,Steven K Hanks,Andres J Garcia
Biology of the Cell null
Focal adhesion kinase modulates radial glia-dependent neuronal migration through connexin-26.
Valiente, M; Ciceri, G; Rico, B; Marin, O
The Journal of Neuroscience null
J Grossmann et al.
Gastroenterology, 120(1), 79-88 (2001-02-24)
Purified intestinal epithelial cells die of detachment-induced apoptosis due to loss of cell anchorage during isolation. Anchorage-dependent cells form focal adhesions, sites of enhanced cell-matrix attachment that confer survival signals. Focal adhesion kinase (FAK), a component of the focal adhesion
c-Yes regulates cell adhesion at the blood-testis barrier and the apical ectoplasmic specialization in the seminiferous epithelium of rat testes.
Xiang Xiao,Dolores D Mruk,Will M Lee,C Yan Cheng
The International Journal of Biochemistry & Cell Biology null
M Zabe et al.
The Journal of biological chemistry, 276(18), 14704-14709 (2001-03-30)
The plasma membrane Ca(2+)-ATPase (PMCA) plays an essential role in maintaining low cytosolic Ca(2+) in resting platelets. During platelet activation PMCA is phosphorylated transiently on tyrosine residues resulting in inhibition of the pump that enhances elevation of Ca(2+). Tyrosine phosphorylation

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