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Anti-FAK Antibody, clone 4.47

clone 4.47, Upstate®, from mouse

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Anti-FADK, Anti-FADK 1, Anti-FAK, Anti-FAK1, Anti-FRNK, Anti-PPP1R71, Anti-p125FAK, Anti-pp125FAK

biological source


Quality Level

antibody form

purified immunoglobulin

antibody product type

primary antibodies


4.47, monoclonal

species reactivity

mouse, rat, human




immunocytochemistry: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable



NCBI accession no.

UniProt accession no.

shipped in

dry ice

target post-translational modification


Gene Information

human ... PTK2(5747)

General description

Focal adhesion kinase (FAK) is a cytoplasmic protein tyrosine kinase that has been implicated to play an important role in integrin-mediated signal transduction pathways. FAK becomes activated and tyrosine phosphorylated in integrin-mediated cell adhesion and is colocalized with integrins and other cytoskeletal proteins in focal contacts in a variety of adherent cells. Physical interactions of FAK with the integrin cytoplasmic domain as well as cytoskeletal proteins talin, paxillin, and/ or tensin have been proposed to play a key role in FAK activation by facilitating its oligomerization and transphosphorylation. FAK shows a rapid increase in tyrosine phosphorylation when stimulated by diverse signaling molecules, including those that regulate embryonic development, cell proliferation, migration and apoptosis, and efficient study methods are in high demand.


Recognizes p125FAK; does not cross react with Pyk-2.


GST fusion protein corresponding to residues 1-423 of human FAK (focal adhesion kinase); specific for p125FAK


Anti-FAK Antibody, clone 4.47 is an antibody against FAK for use in IC, IH, IP & WB.
Research Category
Research Sub Category
Cytoskeletal Signaling


Routinely evaluated by immunoblot on 3T3/A31 RIPA cell lysate, PC-12, Hep-G2, SW 620 or Jurkat RIPA cell lysates

Target description

125 kDa


Replaces: 04-591

Physical form

Format: Purified
Protein G Purified
Protein G Purified immunoglobulin in Immunoaffinity Purified immunoglobulin in 0.2M Tris-glycine, pH 7.4, 0.15M NaCl, 0.1mM EDTA, 0.05% sodium azide before the addition of glycerol to 30%

Storage and Stability

Maintain for 2 years at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Analysis Note

Positive Antigen Control: Catalog #12-305, 3T3/A31 lysate. Add 2.5 μL of 2-mercapto-ethanol/100 μL of lysate and boil for 5 minutes to reduce the preparation. Load 20 μg of reduced lysate per lane for minigels.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany


Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

10 - Combustible liquids



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