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V4505

Sigma-Aldrich

Monoclonal Anti-Vinculin antibody produced in mouse

clone VIN-11-5, ascites fluid

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Synonym(s):
Vinculin Antibody Sigma, Anti-VCL
MDL number:
NACRES:
NA.41

biological source

mouse

Quality Level

conjugate

unconjugated

antibody form

ascites fluid

antibody product type

primary antibodies

clone

VIN-11-5, monoclonal

mol wt

antigen 116 kDa

species reactivity

bovine, human, chicken, mouse

technique(s)

indirect immunofluorescence: 1:50 using cultured chicken fibroblasts
microarray: suitable
western blot: suitable

isotype

IgG1

UniProt accession no.

application(s)

research pathology

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... VCL(7414)
mouse ... Vcl(22330)

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This Item
F7053V9131V9264
conjugate

unconjugated

conjugate

FITC conjugate

conjugate

unconjugated

conjugate

unconjugated

antibody form

ascites fluid

antibody form

purified immunoglobulin

antibody form

ascites fluid

antibody form

purified immunoglobulin

clone

VIN-11-5, monoclonal

clone

hVIN-1, monoclonal

clone

hVIN-1, monoclonal

clone

hVIN-1, monoclonal

mol wt

antigen 116 kDa

mol wt

antigen 116 kDa

mol wt

antigen 116 kDa

mol wt

antigen 116 kDa

species reactivity

bovine, human, chicken, mouse

species reactivity

frog, chicken, mouse, canine, human, bovine, rat, turkey

species reactivity

frog, chicken, mouse, canine, human, bovine, rat, turkey

species reactivity

frog, chicken, mouse, canine, human, bovine, rat, turkey

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General description

Monoclonal Anti-Vinculin (mouse IgG1 isotype) is derived from the hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse.
The vinculin (VCL) gene containing 22 exons is mapped to human chromosome 10q22.1-q23. The gene codes for a 117kDa cytoskeletal protein present in adherens type cell junctions. VCL is a ubiquitously expressed cell-adhesion protein. The protein is characterized with a globular head and a tail domain, which are interconnected by a short proline-rich sequence, an intramolecular interaction between the head and tail conceal many ligand-binding sites in the protein.

Specificity

The antibody reacts best with cultured chicken fibroblasts. Labeling also may be obtained with bovine, human, or mouse cells.

Immunogen

smooth muscle vinculin from chicken gizzard.

Application

Monoclonal Anti-Vinculin antibody produced in mouse has been used in:
  • western blotting.
  • immunofluorescence.
  • immunocytochemistry.

Biochem/physiol Actions

Vinculin (VCL) is a cytoskeletal protein, which plays a vital role in regulation of cell-to-cell and cell to matrix junctions. VCL facilitates normal embryonic development and cardiac function. Aberration or mutation of the gene leads to cardiomyopathy. VCL also plays a crucial role in cell migration and morphogenesis. VCL might facilitate the binding of integrins and cadherins to the actin cytoskeleton. Vinculin plays an important role in integrin αIIbβ3 signaling and function, which is required for platelet aggregation.
Vinculin is suggested to be a possible link between ends of the bundles of actin filaments and the plasma membrane. It has also been proposed that vinculin may be involved in the transmembrane induction of actin bundle formation.

Other Notes

This product can be found as purified product that was produced using cell culture hybridoma product.
SAB4200729 Anti-Vinculin antibody, Mouse monoclonal
clone VIN-11-5, purified from hybridoma cell culture

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

13 - Non Combustible Solids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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E P Moiseyeva et al.
The Journal of biological chemistry, 268(6), 4318-4325 (1993-02-25)
The human vinculin gene contains 22 exons ranging in size from 71 base pairs (bp) to 303 bp (average 155 bp) with the exception of exon 22 which contains 144 bp of coding sequence and 1848 bp of 3'-untranslated sequence
Three-dimensional structure of vinculin bound to actin filaments
Janssen MEW, et al.
Molecular Cell, 21(2), 271-281 (2006)
W Xu et al.
Journal of cell science, 111 ( Pt 11), 1535-1544 (1998-05-15)
Vinculin plays a role in signaling between integrins and the actin cytoskeleton. We reported earlier that F9-derived cells lacking vinculin are less spread, less adhesive, and move two times faster than wild-type F9 cells. Expression of intact vinculin in null
Julia Fröbel et al.
Molecular & cellular proteomics : MCP, 12(5), 1272-1280 (2013-02-06)
Bleeding complications are a significant clinical problem in patients with myelodysplastic syndromes even at sufficient platelet counts (>50,000/μl). However, the underlying pathology of this hemorrhagic diathesis is still unknown. Here, we analyzed the platelet proteome of patients with myelodysplastic syndromes
Meng Chen et al.
The Journal of biological chemistry, 288(48), 34647-34657 (2013-10-24)
The mylk1 gene encodes a 220-kDa nonmuscle myosin light chain kinase (MLCK), a 130-kDa smooth muscle MLCK (smMLCK), as well as the non-catalytic product telokin. Together, these proteins play critical roles in regulating smooth muscle contractility. Changes in their expression

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