ZMS1086
Anti-Vinculin Antibody, clone 7F9 ZooMAb® Mouse Monoclonal
recombinant, expressed in HEK 293 cells
Synonym(s):
Metavinculin
About This Item
biological source
mouse
Quality Level
recombinant
expressed in HEK 293 cells
conjugate
unconjugated
antibody form
purified antibody
antibody product type
primary antibodies
clone
7F9, recombinant monoclonal
product line
ZooMAb® learn more
form
lyophilized
mol wt
calculated mol wt 123.8 kDa
observed mol wt ~125 kDa
purified by
using protein G
species reactivity
rat, human
species reactivity (predicted by homology)
porcine, mouse, monkey, bovine
packaging
antibody small pack of 25 μL
greener alternative product characteristics
Waste Prevention
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Design for Energy Efficiency
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enhanced validation
recombinant expression
Learn more about Antibody Enhanced Validation
sustainability
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technique(s)
immunocytochemistry: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
immunoprecipitation (IP): suitable
western blot: suitable
isotype
IgG1
epitope sequence
Unknown
Protein ID accession no.
UniProt accession no.
greener alternative category
shipped in
ambient
storage temp.
2-8°C
target post-translational modification
unmodified
Gene Information
human ... VCL(7414)
General description
Each ZooMAb® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb® antibodies are reliably available and ready to ship when you need them.
Specificity
Immunogen
Application
Evaluated by Western Blotting in PC12 cell lysate.
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Vinculin in PC12 cell lysate.
Tested applications
Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected Vinculin in HepG2 cell lysate.
Immunoprecipitation Analysis: A representative lot of mouse monoclonal antibody (Clone 7F9) immunoprecipitated Vinculin in Immunoprecipitation applications. (Bays, JL., et al. (2014). J. Cell Biol. 205(2); 251-263).
Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected Vinculin in HepG2 cells.
Immunohistochemistry (Paraffin) Analysis: A 1:1,000 dilution from a representative lot detected Vinculin in human kidney and human colon tissue sections.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Target description
Physical form
Reconstitution
Storage and Stability
Legal Information
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Storage Class
13 - Non Combustible Solids
wgk_germany
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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