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MABF119

Sigma-Aldrich

Anti-EGFR Antibody, clone 528 (Azide-free)

clone 528, from mouse

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Synonym(s):
Epidermal growth factor receptor, Proto-oncogene c-ErbB-1, Receptor tyrosine-protein kinase erbB-1
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

antibody form

purified antibody

antibody product type

primary antibodies

clone

528, monoclonal

species reactivity

human

technique(s)

immunoprecipitation (IP): suitable
neutralization: suitable

isotype

IgG2aκ

NCBI accession no.

UniProt accession no.

shipped in

dry ice

target post-translational modification

unmodified

Gene Information

human ... EGFR(1956)

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MABF120MABS835SAB4500714
vibrant-m

MABS835

Anti-EGFR Antibody, clone 7F12.1

Gene Information

human ... EGFR(1956)

Gene Information

human ... EGFR(1956)

Gene Information

human ... EGFR(1956)

Gene Information

human ... EGFR(1956)

biological source

mouse

biological source

mouse

biological source

mouse

biological source

rabbit

species reactivity

human

species reactivity

human

species reactivity

human

species reactivity

human, rat, mouse

clone

528, monoclonal

clone

225, monoclonal

clone

7F12.1, monoclonal

clone

polyclonal

isotype

IgG2aκ

isotype

IgG1κ

isotype

IgG2bκ

isotype

-

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General description

The epidermal growth factor receptor (EGFR; Proto-oncogene c-ErbB-1) is a ubiquitously receptor tyrosine kinase that is activated by a variety of ligands including EGF, EPGN, TGFA, and EREG. Ligand-bound EGFR undergoes dimerization and autophosphorylation exposing cytoplasmic domains which allow interaction with adaptor proteins and induction of several signaling pathways including PI3-AKT; PLCγ-PKC; Notch1; and Ras-Raf. EGFR therefore contributes to a diverse range of cellular processes inlcuding cell proliferation. Several studies have reported that EGFR signaling plays an important role in the development of various tumors. EGFR is regulated by PTPRJ and PTPRK phosphatases, and by the endocytic machinery including EPS15 that mediates internalization and degradation of the receptor.

Specificity

This antibody recognizes the extracellular domain of EGFR.

Immunogen

Partially purified human EGFR from A431 cells

Application

Anti-EGFR Antibody, clone 528 (Azide-free) detects level of EGFR & has been published & validated for use in Immunoprecipitation, Neutralizing.
Neutralizing Assay Analysis: A representative lot was used by an independent laboratory in A431 cells. (Kawamoto, T., et al. (1984). Journal of Biological Chemistry. 259(12):7761-7766.)

Quality

Evaluated by Immunoprecipitation in A431 cell lysate.

Immunoprecipitation Analysis: 10 µg of this antibody immunoprecipitated EGFR from A431 cell lysate.

Target description

~170 kDa observed. Uniprot gives a calculated molecular weight of 135 but can be observed at ~170 kDa due to phosphorylation. (Konishi, A., et al. (2003). The Journal of Biological Chemistry. 278(37):35049–35056.)

Linkage

Replaces: 04-338

Physical form

Format: Purified

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable


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Valencio Salema et al.
mAbs, 8(7), 1286-1301 (2016-07-30)
Most therapeutic antibodies (Abs) target cell surface proteins on tumor and immune cells. Cloning of Ab gene libraries in E. coli and their display on bacteriophages is commonly used to select novel therapeutic Abs binding target antigens, either purified or
Antoine Lesur et al.
Proteomics. Clinical applications, 9(7-8), 695-705 (2015-02-07)
We report an immunocapture strategy to extract proteins known to harbor driver mutations for a defined cancer type before the simultaneous assessment of their mutational status by MS. Such a method bypasses the sensitivity and selectivity issues encountered during the

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