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MABF2159

Sigma-Aldrich

Anti-CD63 (LAMP3) Antibody, clone ME491

clone ME491, from mouse

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Synonym(s):
CD63 antigen, Granulophysin, Lysosomal-associated membrane protein 3, LAMP-3, Melanoma-associated antigen ME491, OMA81H, Ocular melanoma-associated antigen, Tetraspanin-30, Tspan-30
eCl@ss:
32160702

biological source

mouse

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

ME491, monoclonal

species reactivity

human

packaging

antibody small pack of 25 μg

technique(s)

flow cytometry: suitable
immunocytochemistry: suitable
immunohistochemistry: suitable (paraffin)
western blot: suitable

isotype

IgG1κ

NCBI accession no.

UniProt accession no.

target post-translational modification

unmodified

Gene Information

human ... CD63(967)

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vibrant-m

SAB4301607

Anti-CD63

Gene Information

human ... CD63(967)

Gene Information

human ... CD63(967)

Gene Information

human ... CD63(967)

Gene Information

human ... CD63(967)

clone

ME491, monoclonal

clone

polyclonal

clone

MEM-259, monoclonal

clone

polyclonal

biological source

mouse

biological source

rabbit

biological source

mouse

biological source

rabbit

antibody form

purified immunoglobulin

antibody form

affinity isolated antibody

antibody form

purified immunoglobulin

antibody form

affinity isolated antibody

NCBI accession no.

NP_001244318

NCBI accession no.

-

NCBI accession no.

NM_001040034.1

NCBI accession no.

-

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General description

CD63 antigen (UniProt: P08962; also known as Granulophysin, Lysosomal-associated membrane protein 3, LAMP-3, Melanoma-associated antigen ME491, OMA81H, Ocular melanoma-associated antigen, Tetraspanin-30, Tspan-30, CD63) is encoded by the CD63 (also known as MLA1, TSPAN30) gene (Gene ID: 967) in human. CD63 is a multi-pass membrane protein of the tetraspan family that is found on endosome, lysosome, and plasma membranes. CD63 has been detected in platelets, Dysplastic nevi benign moles), radial growth phase primary melanomas, hematopoietic cells, and in tissue macrophages. In melanoma cells it is involved in their motility and adhesion. CD63 also plays a role in the adhesion of leukocytes onto endothelial cells. It is reported to play a role in the activation of ITGB1 and integrin signaling, leading to the activation of AKT, FAK/PTK2 and MAP kinases and promote cell survival, reorganization of the actin cytoskeleton, cell adhesion, spreading and migration. CD63 is a highly N-glycosylated protein with three asparagine glycosylation sites (aa 130, 150, 172) and its ribophorin II (RPN2)-mediated glycosylation has been linked to breast cancer. Overexpression of CD63 has been observed in esophageal cancer that is negatively correlated with tumor stage and lymph node metastasis. Lack of expression of CD63 in platelets has been observed in a patient with Hermansky-Pudlak syndrome (HPS), an autosomal recessive disorder that is characterized by oculocutaneous albinism, bleeding due to platelet storage pool deficiency, and lysosomal storage defects. This antibody (clone ME491) is shown to react with human primary and to some extent with metastatic melanoma tissues. (Ref.: Lai, X., et al. (2017). Oncol. Let. 13(6); 4245-4251; Tominaga, N., et al. (2014). Mol. Cancer 13; 134; Smith, M., et al. (1997). Melanoma Res. 7 (Suppl. 2), 163-170).

Specificity

Clone ME491 specifically detects CD63 (LAMP-3) in human cells.

Immunogen

Clear supernatant from SK-Mel-23 cell lysate.

Application

Anti-CD63 (LAMP3), clone ME491, Cat. No. MABF2159, is a mouse monoclonal antibody that detects CD63 antigen and has been tested for use in Flow Cytometry, Immunocytochemistry, Immunohistochemistry (Paraffin), and Western Blotting.
Immunohistochemistry (Paraffin) Analysis: A 1:250 dilution from a representative lot detected CD63 (LAMP3) in human spleen and human bone marrow tissue sections.

Immunocytochemistry Analysis: A representative lot detected CD63 (LAMP3) in Immunocytochemistry applications (Atkinson, B., et. al. (1984). Cancer Res. 44(6):2577-81).

Flow Cytometry Analysis: A representative lot detected CD63 (LAMP3) in Flow Cytometry applications (Li, J., et. al. (2003). J Immunol. 171(6):2922-9).

Western Blotting Analysis: A representative lot detected CD63 (LAMP3) in Western Blotting applications (Smith, M., et. al. (1997). Melanoma Res. 7 Suppl 2:S163-70).

Immunohistochemistry Analysis: A representative lot detected CD63 (LAMP3) in Immunohistochemistry applications (Li, J., et. al. (2003). J Immunol. 171(6):2922-9).
Research Category
Inflammation & Immunology

Quality

Evaluated by Western Blotting in human platelet lysate.

Western Blotting Analysis: 2 µg/mL of this antibody detected CD63 (LAMP3) in human platelet lysate.

Target description

~43 kDa observed; 25.64 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

Physical form

Format: Purified
Protein G purified
Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Other Notes

Concentration: Please refer to lot specific datasheet.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Philipp Rhode et al.
Cancers, 13(23) (2021-12-11)
In-depth characterization has introduced new molecular subtypes of gastric cancer (GC). To identify these, new approaches and techniques are required. Liquid biopsies are trendsetting and provide an easy and feasible method to identify and to monitor GC patients. In a

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