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CBL553

Sigma-Aldrich

Anti-CD63 Antibody, clone RFAC4

clone RFAC4, Chemicon®, from mouse

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Synonym(s):
Anti-LAMP-3, Anti-ME491, Anti-MLA1, Anti-OMA81H, Anti-TSPAN30
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

antibody form

purified antibody

antibody product type

primary antibodies

clone

RFAC4, monoclonal

species reactivity

human

manufacturer/tradename

Chemicon®

technique(s)

flow cytometry: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
immunoprecipitation (IP): suitable
western blot: suitable

isotype

IgG1

NCBI accession no.

UniProt accession no.

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... CD63(967)

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This Item
SAB4700217SAB4301607SAB4700218
vibrant-m

CBL553

Anti-CD63 Antibody, clone RFAC4

vibrant-m

SAB4301607

Anti-CD63

biological source

mouse

biological source

mouse

biological source

rabbit

biological source

mouse

species reactivity

human

species reactivity

human

species reactivity

human, mouse

species reactivity

human

Gene Information

human ... CD63(967)

Gene Information

human ... CD63(967)

Gene Information

human ... CD63(967)

Gene Information

human ... CD63(967)

clone

RFAC4, monoclonal

clone

MEM-259, monoclonal

clone

polyclonal

clone

MEM-259, monoclonal

technique(s)

flow cytometry: suitable, immunoprecipitation (IP): suitable, immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable, western blot: suitable

technique(s)

flow cytometry: suitable

technique(s)

immunohistochemistry: 1:25-1:100, western blot: 1:500-1:2000

technique(s)

flow cytometry: suitable

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Specificity

This antibody reacts with a 53 kDa protein which form a part of the family of tetraspan moieties. The antigen was originally designated as a lysosomal membrane protein characterized as an activation dependent platelet surface antigen. In fact the CD63 antigen has a diverse distribution on the surface and in the cytoplasm of many cell types including lymphoid, myeloid, endothelial cells and melanoma.

Application

Anti-CD63 Antibody, clone RFAC4 is an antibody against CD63 for use in FC, IP, WB, IH, IH(P).
Flow cytometric studies of CD63 expression

Immunoprecipitation

Western blotting of CD63 antigen from solubilized platelet proteins

Suitable for formalin fixed paraffin wax embedded tissue sections. HIER and proteolytic pretreatment may not be necessary. Users should confirm specificity and pretreatment for their particular application.

Optimal working dilutions must be determined by the end user.
Research Category
Inflammation & Immunology
Research Sub Category
Immunoglobulins & Immunology

Physical form

Format: Purified
The monoclonal is presented at a concentration of 100μg/1ml in phosphate buffered saline containing 10mM sodium azide and 1mg/ml bovine serum albumin. We recommend that each laboratory determine an optimum working titre for use in its particular application.

Storage and Stability

For use within 1 month of purchase store at +4°C, for long term storage aliquot antibody into small volumes and store at -20°C.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable


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