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MABF3068

Sigma-Aldrich

Anti-CD163 Antibody, clone MAC2-158

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Synonym(s):
Scavenger receptor cysteine-rich type 1 protein M130;Hemoglobin scavenger receptor

biological source

mouse

Quality Level

conjugate

unconjugated

antibody form

purified antibody

antibody product type

primary antibodies

clone

MAC2-158, monoclonal

mol wt

calculated mol wt 125.45 kDa

purified by

using protein G

species reactivity

human

packaging

antibody small pack of 100 μg

technique(s)

flow cytometry: suitable
immunocytochemistry: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
immunoprecipitation (IP): suitable
western blot: suitable

isotype

IgG1κ

epitope sequence

N-terminal

Protein ID accession no.

UniProt accession no.

shipped in

ambient

target post-translational modification

unmodified

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This Item
SAB5500046SAB1301555MABF853
conjugate

unconjugated

conjugate

unconjugated

conjugate

unconjugated

conjugate

-

biological source

mouse

biological source

rabbit

biological source

rabbit

biological source

hamster (Armenian)

species reactivity

human

species reactivity

human (tested)

species reactivity

human

species reactivity

mouse

antibody form

purified antibody

antibody form

affinity isolated antibody

antibody form

IgG fraction of antiserum

antibody form

purified antibody

UniProt accession no.

Q86VB7

UniProt accession no.

P08637

UniProt accession no.

Q86VB7

UniProt accession no.

A0A0B4J1G0

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General description

Scavenger receptor cysteine-rich type 1 protein M130 (UniProt: Q86VB7; also known as Hemoglobin scavenger receptor, CD163) is encoded by the CD163 (also known as M130) gene (Gene ID: 9332) in human. CD163 is a single-pass type I membrane protein that is synthesized with a signal peptide (aa 1-41), which is subsequently cleaved off to produce the mature form that contains a long extracellular domain (aa 42-1050), a transmembrane domain (aa 1051-1071), and a cytoplasmic domain (aa 1072-1156). The extracellular domain contains nine scavenger receptor cysteine-rich (SRCR) scavenger receptor class B domains of about 100 amino acids each. SRCR domain 3 mediates calcium-sensitive interaction with Hemoglobin/Haptoglobin (Hb/Hp) complexes. CD163 is expressed in monocytes and mature macrophages with high expression observed in bone marrow macrophages, Kupffer cells, lung macrophages, and red pulp macrophages. Its levels are up regulated by anti-inflammatory mediators and suppressed by pro-inflammatory mediators like lipopolysaccharides (LPS) and TNF . It serves as an acute phase-regulated receptor involved in clearance and endocytosis of Hb/Hp complexes by macrophages and protects tissues from free hemoglobin-mediated oxidative damage. The binding of the Hb/Hp complex to CD163 macrophages leads to a fast degradation of the complex. CD163 also plays a role in the uptake and recycling of iron, via endocytosis of Hb/Hp complex and subsequent breakdown of heme. A soluble form of CF163 (sCD163) has also been described that is produced by proteolytic shedding and can be induced by LPS, phorbol ester, and Fc region of immunoglobulin . This soluble form plays an anti-inflammatory role and is considered a diagnostic parameter for monitoring macrophage activation in inflammatory conditions. Its expression is markedly induced by anti- inflammatory mediators, such as glucocorticoids and interleukin 10. Clone MAC2-158 binds to SRCR domain 1 of CD163 and recognizes over 80% of circulating CD14-expressing monocytes. (Ref.: Zhi, Y., et al. (2017). Mol. Med. Rep. 15(5); 2931-2939; Maniecki, MB., et al. (2011). Immunology 216(8); 882-890; Morganelli, PM., and Guyre, PM. (1988). J. Immunol. 140(7); 2296-2304).

Specificity

Clone MAC2-158 is a mouse monoclonal antibody that detects human CD163. It targets an epitope within the SRCR1 domain from the N-terminal region.

Immunogen

Interferon and Dexamethasone treated human monocytes.

Application

Quality Control Testing

Evaluated by Immunohistochemistry (Paraffin) in human thymus tissue sections.

Immunohistochemistry (Paraffin) Analysis: A 1:250 dilution of this antibody detected CD163 in human thymus tissue sections.

Tested Applications

Immunohistochemistry (Paraffin) Analysis: A 1:250 dilution from a representative lot detected CD163 in human liver tissue sections.

Flow Cytometry Analysis: A representative lot detected CD163 in Flow Cytometry applications Maniecki, M.B., et al. (2011). Immunobiology. 216(8): 882-90; Krijgsman, D., et al. (2020). Int J Mol Sci. 21(16): 5925).

Immunocytochemistry Analysis: A representative lot detected CD163 in Immunocytochemistry applications (Maniecki, M.B., et al. (2011). Immunobiology. 216(8): 882-90).

Flow Cytometry Analysis: A representative lot detected CD163 in human peripheral blood mononuclear cells (PBMC) (Data courtesy of Jane Collins, Research Laboratory Manager of Dr. Paul M. Guyre s Lab, Department of Microbiology and Immunology, Geisel School of Medicine, Dartmouth College, NH, USA).

Western Blotting Analysis: A representative lot detected CD163 in Western Blotting applications (Maniecki, M.B., et al. (2011). Immunobiology. 216(8): 882-90).

Immunoprecipitation Analysis: A representative lot immunoprecipitated CD163 in Immunoprecipitation applications (Morganelli, P.M., et al. (1988). J Immunol. 140(7): 2296-304).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
This mouse monoclonal Anti-CD163, clone MAC2-158, Cat. No. MABF3068 is tested for use in Immunohistochemistry (Paraffin), Immunoprecipitation, Western Blotting, Flow Cytometry, Immunocytochemistry, for the detection of CD163.

Physical form

Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Recommended storage: +2°C to +8°C.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable


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