MilliporeSigma
All Photos(1)

Documents

MABF938

Sigma-Aldrich

Anti-MxA, clone M143 (CL143)

clone CL143, from mouse

Sign Into View Organizational & Contract Pricing

Synonym(s):
MxA, IFI-78K, Interferon-induced GTP-binding protein Mx1, Interferon-induced protein p78, Myxoma resistance protein 1, Myxovirus resistance 1, MX1
eCl@ss:
32160702

biological source

mouse

Quality Level

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

CL143, monoclonal

species reactivity

guinea pig, human, mouse, rat

technique(s)

flow cytometry: suitable
immunohistochemistry: suitable (paraffin)
western blot: suitable

isotype

IgGκ

NCBI accession no.

UniProt accession no.

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... MX1 (4599)

Related Categories

General description

Interferon-induced GTP-binding protein Mx1 (UniProt P20591; also known as IFI-78K, Interferon-induced protein p78, Interferon-inducible protein p78, Interferon-regulated resistance GTP-binding protein MxA, Myxoma resistance protein 1, Myxovirus resistance 1) is encoded by the MX1 (also known as MX, IFI78) gene (Gene ID 4599) in human. The interferon-inducible myxovirus resistance (Mx) proteins belong to the family of large GTPases and are highly homologous with dynamins within their GTP-binding domain. Mx proteins differ from small GTPases and heterotrimeric G proteins in features such as their large size (70–100 kDa), a relatively low affinity for GTP, and a high intrinsic rate of GTP hydrolysis. Mx proteins contain a highly conserved tripartite GTP-binding motif within the N-terminal G domain, while their less conserved C-terminal half serves different functions such as homooligomerization and association with binding partners. Two distinct regions of human MxA, a central interactive region (amino acids 372–540) and a C-terminal leucine zipper motif (amino acids 564–662), are responsible for intra- and intermolecular interactions. MxA/Mx1 is cytosolic, while two MxB/Mx forms exist, a 78 kDa nuclear form and a 76 kDa cytosolic form lacking the N-terminal nuclear localization signal (NLS). Mx proteins are induced by type I IFNs and possess important antiviral properties. Human MxA and rodent MxB (Mx2), in particular, confer resistance against influenza virus and hantaviruses, including Seoul virus, Puumala virus, Hantaan virus, and Andes virus, in vitro. Human MxB is also reported to inhibit HIV-1 infection by reducing the level of integrated viral DNA.

Specificity

Clone M143 (a.k.a. CL143) is expected to react with both the canonical MxA (p78; Mx1) and the spliced variant varMxA (p56). This clone is also reported to cross-react with rat Mx2 and Mx3 (Hannah, M.F., et al. (2008). Brain Behav Immun. 22(4):503-516).

Immunogen

Epitope: N-terminal half
His-tagged recombinant protein corresponding to full-length human MxA.

Application

Immunohistochemistry Analysis: A representative lot detected upregulated MxA in cells showing positive viral nucleocapsid protein (NP) immunoreactivity in rat lung tissue 40 days post intraperitoneal Seoul virus inoculation by fluorescent immunohistochemistry using paraffin-embbeded sections (Hannah, M.F., et al. (2008). Brain Behav Immun. 22(4):503-516).
Immunohistochemistry Analysis: Representative lots detected MxA immunoreactivity in patients-derived skin lesion samples using paraffin-embedded tissue sections (Urosevic, M., et al. (2007). J. Clin. Invest. 117(10): 2834–2846; Urosevic, M., et al. (2005). J. Natl. Cancer Inst. 97(15):1143-1153).
Flow Cytometry Analysis: A representative lot detected MxA expression in MxA-transfected U-87-H4 and U-87-D11, but not untransfected U-87-K4 human gliobastoma cells (Schneider-Schaulies, S., et al. (1994). J. Virol. 68(11):6910-6917).
Western Blotting Analysis: A representative lot detected a robust MxA induction in the lung tissue from guinea pigs that recieved intranasal administration of recombinant human alpha-IFN (Van Hoeven, N., et al. (2009). J. Virol. 83(7): 2851–2861).
Western Blotting Analysis: A representative lot detected a time-dependent MxA induction in the lung tissue from rats following intraperitoneal Seoul virus inoculation (Hannah, M.F., et al. (2008). Brain Behav Immun. 22(4):503-516).
Immunoprecipitation Analysis: A representative lot immunoprecipitated MxA from murine MxA-expressing Swiss 3T3 cells (clone 4.5.15) (Flohr, F., et al. (1999). FEBS Lett. 463(1-2):24-28).
Research Category
Inflammation & Immunology
Research Sub Category
Immunoglobulins & Immunology
This Anti-MxA, clone M143 (CL143) is validated for use in Western Blotting, Immunohistochemistry (Paraffin) and Flow Cytometry for the detection of MxA.

Quality

Evaluated by Western Blotting in Swiss 3T3 (clone 4.5.15) cell lysate.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected MxA in 10 µg of Swiss 3T3 (clone 4.5.15) cell lysate.

Target description

~75 kDa observed

Physical form

Format: Purified
Protein G Purified
Purified mouse monoclonal IgGκ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Other Notes

Concentration: Please refer to lot specific datasheet.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Documents related to the products that you have purchased in the past have been gathered in the Document Library for your convenience.

Visit the Document Library

Difficulty Finding Your Product Or Lot/Batch Number?

Product numbers are combined with Pack Sizes/Quantity when displayed on the website (example: T1503-25G). Please make sure you enter ONLY the product number in the Product Number field (example: T1503).

Example:

T1503
Product Number
-
25G
Pack Size/Quantity

Additional examples:

705578-5MG-PW

PL860-CGA/SHF-1EA

MMYOMAG-74K-13

1000309185

enter as 1.000309185)

Having trouble? Feel free to contact Technical Service for assistance.

Lot and Batch Numbers can be found on a product's label following the words 'Lot' or 'Batch'.

Aldrich Products

  • For a lot number such as TO09019TO, enter it as 09019TO (without the first two letters 'TO').

  • For a lot number with a filling-code such as 05427ES-021, enter it as 05427ES (without the filling-code '-021').

  • For a lot number with a filling-code such as STBB0728K9, enter it as STBB0728 without the filling-code 'K9'.

Not Finding What You Are Looking For?

In some cases, a COA may not be available online. If your search was unable to find the COA you can request one.

Request COA

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service