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MABN1573

Sigma-Aldrich

Anti-Synapsin-2 Antibody, clone 19.4

clone 19.4, from mouse

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Synonym(s):
Synapsin II, Synapsin-2
eCl@ss:
32160702

biological source

mouse

Quality Level

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

19.4, monoclonal

species reactivity

rat, mouse, human

technique(s)

activity assay: suitable
immunohistochemistry: suitable (paraffin)
immunoprecipitation (IP): suitable
western blot: suitable

isotype

IgG2aκ

NCBI accession no.

UniProt accession no.

shipped in

dry ice

target post-translational modification

unmodified

Gene Information

human ... SYN2(6854)

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MABN1584MABN894AB1543P
Anti-Synapsin I Antibody Chemicon®, from rabbit

AB1543P

Anti-Synapsin I Antibody

clone

19.4, monoclonal

clone

19.21, monoclonal

clone

10.22, monoclonal

clone

polyclonal

antibody form

purified immunoglobulin

antibody form

purified immunoglobulin

antibody form

purified antibody

antibody form

affinity purified immunoglobulin

species reactivity

rat, mouse, human

species reactivity

rat, human, mouse

species reactivity

rat, mouse, human, fish, bovine

species reactivity

human, mouse, rat, bovine

Gene Information

human ... SYN2(6854)

Gene Information

human ... SYN2(6854)

Gene Information

human ... SYN1(6853)

Gene Information

human ... SYN1(6853)

UniProt accession no.

Q63537

UniProt accession no.

Q63537

UniProt accession no.

P17599

UniProt accession no.

P17600

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General description

Synapsin-2 (UniProt Q63537; also known as Synapsin II) is encoded by the Syn2 gene (Gene ID 29179) in rat species. The synapins constitute a family of abundant neuronal phosphoproteins that regulate SV trafficking and neurotransmitter release at the pre-synaptic terminal. Three genes exisit in mammals, encoding altogether 11 synapsin members by alternative splicings (Synapsin Ia & Ib by SYN1, Synapsin IIa & IIb by SYN2, Synapsin IIIa through IIIf by SYN3), Syn III is the most precociously expressed isoform that has a role in the early phases of neural development and is downregulated in mature neurons. On the other hand, Syn I and Syn II are expressed at low levels at birth and their expressions progressively increase during synaptogenesis to reach a stable plateau at 1–2 months of life. The NH2-terminal region is divided in A, B, and C domains and is highly conserved among synapsin isoforms. Synapsins are regulated by postransloational phosphorylations. Domain A contains PKA and CaMKI/IV phosphorylation sites, domain B contains MAPK/Erk phosphorylation sites, and domain C is phosphorylated by Src. The c-terminal regions contain spliced domains and diverge among synapsin isoforms (domain D in Syn Ia and Ib, domain G in Syn IIa and IIb, domain H in Syn IIa, and domain J in Syn IIIa), although they all bear proline-rich regions binding to several SH3-containing proteins and additional phosphorylation sites for CaMKII, MAPK/Erk, and cdk1/5.

Specificity

Clone 19.4 reacts with both synapsin-2 spliced isoforms (IIa and IIb). Clone 19.4 does not react with any of the two synapsin-1 spliced isoforms (Vaccaro, P., et al. (1997). Brain Res. Mol. Brain Res. 52(1):1-16).

Immunogen

Epitope: Domains A&B
Purified recombinant rat synapsin-2.

Application

Anti-Synapsin-2 Antibody, clone 19.4 is an antibody against Synapsin-2 for use in Western Blotting, Immunohistochemistry (Paraffin), Immunoprecipitation, Enzyme Assay.
Immunohistochemistry Analysis: A 1:1,000 dilution from a representative lot detected Synapsin-2 in human cerebral cortex, mouse cerebellum, and rat cerebral cortex tissue.
Western Blotting Analysis: Target detection by a representative lot was blocked by a preincubation with phage particles displaying peptides containing the SYIxN consensus sequence (Vaccaro, P., et al. (1997). Brain Res. Mol. Brain Res. 52(1):1-16).
Western Blotting Analysis: A representative lot detected synapsin IIa/IIb, but not Ia/Ib, in rat brain cytosolic extracts (Vaccaro, P., et al. (1997). Brain Res. Mol. Brain Res. 52(1):1-16).
Immunoprecipitation Analysis: A representative lot immunoprecipitated synapsin IIa/IIb, but not Ia/Ib, from rat brain synaptosomal preparations (Vaccaro, P., et al. (1997). Brain Res. Mol. Brain Res. 52(1):1-16).
Enzyme Assay Analysis: A representative lot, when added 2 hours prior to cAMP, prevented PKA-catalyzed phosphorylation of synapsin IIa/IIb in an in vitro kinase assay using rat brain synaptosomal preparations (Vaccaro, P., et al. (1997). Brain Res. Mol. Brain Res. 52(1):1-16).
Research Category
Neuroscience
Research Sub Category
Developmental Neuroscience

Quality

Evaluated by Western Blotting in rat brain tissue lysate.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected Synapsin-2 in 10 µg of rat brain tissue lysate.

Target description

~63 and 52 kDa observed

Physical form

Format: Purified
Protein G Purified
Purified mouse monoclonal IgG2aκ antibody in PBS without preservatives.

Storage and Stability

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Other Notes

Concentration: Please refer to lot specific datasheet.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable


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Kalpana D Acharya et al.
Journal of neuroendocrinology, 32(10), e12904-e12904 (2020-10-02)
Neural progestin receptors (PR) function in reproduction, neural development, neuroprotection, learning, memory and the anxiety response. In the absence of progestins, PR can be activated by dopamine (DA) in the rodent hypothalamus to elicit female sexual behaviour. The present study
Kalpana D Acharya et al.
eNeuro, 4(5) (2017-09-29)
Progestins bind to the progestin receptor (PR) isoforms, PR-A and PR-B, in brain to influence development, female reproduction, anxiety, and stress. Hormone-activated PRs associate with multiple proteins to form functional complexes. In the present study, proteins from female mouse hypothalamus

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