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MABN763

Sigma-Aldrich

Anti-Ubiquilin 2 Antibody, clone 6H9

clone 6H9, from mouse

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Synonym(s):
Ubiquilin-2, Chap1, DSK2 homolog, Protein linking IAP with cytoskeleton 2, PLIC-2, hPLIC-2, Ubiquitin-like product Chap1/Dsk2
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

6H9, monoclonal

species reactivity

human, mouse

technique(s)

immunocytochemistry: suitable
western blot: suitable

isotype

IgG1κ

NCBI accession no.

UniProt accession no.

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... UBQLN2(29978)

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WH0029978M3SAB4301925HPA006431
vibrant-m

SAB4301925

Anti-UBQLN2

Gene Information

human ... UBQLN2(29978)

Gene Information

human ... UBQLN2(29978)

Gene Information

human ... UBQLN2(29978)

Gene Information

human ... UBQLN2(29978)

biological source

mouse

biological source

mouse

biological source

rabbit

biological source

rabbit

species reactivity

human, mouse

species reactivity

rat, mouse, human

species reactivity

human, mouse

species reactivity

human

clone

6H9, monoclonal

clone

5F5, monoclonal

clone

polyclonal

clone

polyclonal

technique(s)

immunocytochemistry: suitable, western blot: suitable

technique(s)

indirect ELISA: suitable, indirect immunofluorescence: suitable, western blot: 1-5 μg/mL

technique(s)

immunohistochemistry: 1: 30-150, western blot: 1:500-1:2000

technique(s)

immunoblotting: 0.04-0.4 μg/mL, immunofluorescence: 0.25-2 μg/mL, immunohistochemistry: 1:50-1:200

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General description

Ubiquilin-2, also known as Chap1, DSK2 homolog, Protein linking IAP with cytoskeleton 2 (PLIC-2 or hPLIC-2), Ubiquitin-like product Chap1/Dsk2, and encoded by the gene UBQLN2/N4BP4/PLIC2, is a protein that appears to modulate the half-life of proteins destined to be degraded by the proteasome. Ubiquilin 2 binds UBE3A and BTRC ubquitin ligases and interacts with the 19S proteasome subunit to mediate its activity. Ubiquilin 2 is localized to the cytoplasm and nucleus, and colocalizes with proteasome. Ubiquilin 2 is expressed in mitotic cells very strongly; expression is low in non-dividing cells. Defects in Ubiquilin 2 may be associated with ALS form 15 a neurodegenerative disorder affecting upper motor neurons in the brain and lower motor neurons in the brain stem and spinal cord, resulting in fatal paralysis.

Immunogen

Recombinant protein corresponding to human Ubiquilin 2.

Application

Detect Ubiquilin-2 using this mouse monoclonal antibody, Anti-Ubiquilin 2 Antibody, clone 6H9 validated for use in western blotting & ICC.
Research Category
Neuroscience
Research Sub Category
Developmental Signaling
Western Blotting Analysis: 1.0 µg/mL from a representative lot detected Ubiquilin 2 in 10 µg of SH-SY5Y and NIH-3T3 cell lysate.
Immunocytochemistry Analysis: A representative lot detected Ubiquilin 2 in HeLa cells.

Quality

Evaluated by Western Blotting in U-251 cell lysate.

Western Blotting Analysis: 1.0 µg/mL of this antibody detected Ubiquilin 2 in 10 µg of U-251 cell lysate.

Target description

~66 kDa observed

Physical form

Format: Purified
Protein G Purified
Purified mouse monoclonal IgG1κ in buffer containing PBS, 50% glycerol, and 0.05% sodium azide.

Storage and Stability

Stable for 1 year at -20°C from date of receipt.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

10 - Combustible liquids

wgk_germany

WGK 2


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Oscar Jara et al.
Molecular vision, 26, 204-215 (2020-03-28)
p62/Sequestosome 1 (p62) is a stress-induced protein that is involved in several different intracellular pathways, including regulation of aspects of protein degradation. p62 levels are elevated in several types of cataracts. We investigated whether levels of p62 and its phosphorylation
Aya Jodo et al.
Frontiers in immunology, 11, 1559-1559 (2020-08-28)
Activation of NF-κB transcription factors is critical for innate immune cells to induce inflammation and fight against microbial pathogens. On the other hand, the excessive and prolonged activation of NF-κB causes massive inflammatory damage to the host, suggesting that regulatory

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