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NS225

Sigma-Aldrich

Neurite Outgrowth Assay Kit (1 µm)

The NS225 Neurite Outgrowth Assay Kit (1 μm) is based on the use of Millicell cell culture inserts (chambers) containing a permeable membrane with 1 μm pores at the base.

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32161000

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species reactivity (predicted by homology)

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manufacturer/tradename

Chemicon®

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cell based assay: suitable

detection method

colorimetric

shipped in

wet ice

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NS220ECM810IMM001
species reactivity (predicted by homology)

all

species reactivity (predicted by homology)

all

species reactivity (predicted by homology)

-

species reactivity (predicted by homology)

-

manufacturer/tradename

Chemicon®

manufacturer/tradename

Chemicon®

manufacturer/tradename

Chemicon®

manufacturer/tradename

Chemicon®

detection method

colorimetric

detection method

colorimetric

detection method

colorimetric

detection method

-

shipped in

wet ice

shipped in

wet ice

shipped in

-

shipped in

dry ice

Quality Level

100

Quality Level

100

Quality Level

100

Quality Level

100

General description

Application CHEMICON′s NS225 Neurite Outgrowth Assay Kit (1 μm) utilizes microporous tissue culture insert technology from Millipore and is based on the use of Millicell cell culture inserts (chambers) containing a permeable membrane with 1 μm pores at the base. The inserts are designed to fit into a receiver vessel, which when in use contains differentiation media in contact with the bottom of the insert membrane. The permeable membranes allow for discrimination between neurites and cell bodies, as projecting neurites will pass easily through the pores but cell bodies will not. Therefore, by inducing neurites to traverse these membrane pores, a purified population of neurites is located on the underside of the membrane, distal to the surface on which neural cell bodies are deposited. This kit has has been specifically designed for and validated with PC12 cells. The diameter of PC12 cell bodies is relatively small compared to that of many other cell types commonly used in neurite outgrowth assays [5, 6, 7]. As a result, many PC12 cell bodies may pass though pores larger than 1 μm, significantly obscuring neurite outgrowth assay results. Using a 1 μm pore size greatly reduces background and improves the signal-to-noise ratio in studies using PC12 cells. Use of this 1 μm pore size with larger cell types that extend wider diameter neurites, e.g., N1E-115 cells, will greatly diminish the number of neurites able to project through to the underside of the membrane, and is thus not recommended. The assay is a simple, efficient, and versatile system for the quantitative determination of compounds that influence neurite formation and repulsion. With this system, it is possible to screen numerous biological and pharmacological agents simultaneously, directly evaluate adhesion and guidance receptor functions responsible for neurite extension and repulsion, as well as analyze gene function in transfected cells. The microporous filter allows for biochemical separation and purification of neurites and cell bodies for detailed molecular analysis of protein expression, signal transduction processes and identification of drug targets that regulate neurite outgrowth or retraction processes. Reagents and materials supplied in the NS225 Neurite Outgrowth Assay Kit are sufficient for 12 tests.

Application

The NS225 Neurite Outgrowth Assay Kit (1 μm) is based on the use of Millicell cell culture inserts (chambers) containing a permeable membrane with 1 μm pores at the base.

Components

Neurite Outgrowth Plate Assembly, 1 µm: (Part No. 2007254) 1 x 24-well plate containing 12 x Millicell hanging inserts with 1 µm pore size membranes.

Neurite Stain Solution: (Part No. 90242) One 20 mL bottle.

Neurite Stain Extraction Buffer: (Part No. 90243) One 20 mL bottle.

Neurite Outgrowth Assay Plate: (Part No. 2007255) Two 24-well plates.

Cotton Swabs: (Part No. 10202) 50 swabs.

Forceps: (Part No. 10203) One each.

Storage and Stability

When stored at 2-8° C, the kit components are stable up to the expiration date. Do not freeze or expose to elevated temperatures. Discard any remaining reagents after the expiration date.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Hazard Classifications

Eye Irrit. 2 - Flam. Liq. 2

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3 - Flammable liquids

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WGK 1


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Yue Tang et al.
Life sciences, 91(19-20), 951-958 (2012-09-25)
It is well known that neural stem/progenitor cells (NS/PC) are an ideal cell type for the treatment of central nervous system (CNS) disease. However, ethical problems have severely hampered fetal NS/PC from being widely used as a source for stem
Jin-Mu Yi et al.
Molecules (Basel, Switzerland), 24(6) (2019-04-03)
The dried fruits of Forsythia viridissima have been prescribed to relive fever, pain, vomiting, and nausea in traditional medicine. Oxaliplatin (LOHP) is used to treat advanced colorectal cancer; however, it frequently induces peripheral neuropathies. This study was done to evaluate
D G Drubin et al.
The Journal of cell biology, 101(5 Pt 1), 1799-1807 (1985-11-01)
Nerve growth factor (NGF) regulates the microtubule-dependent extension and maintenance of axons by some peripheral neurons. We show here that one effect of NGF is to promote microtubule assembly during neurite outgrowth in PC12 cells. Though NGF causes an increase
P Lamoureux et al.
The Journal of cell biology, 110(1), 71-79 (1990-01-01)
Several groups have shown that PC12 will extend microtubule-containing neurites on extracellular matrix (ECM) with no lag period in the absence of nerve growth factor. This is in contrast to nerve growth factor (NGF)-induced neurite outgrowth that occurs with a
Jin-Mu Yi et al.
BMC complementary and alternative medicine, 19(1), 339-339 (2019-12-01)
The dried fruits of Forsythia suspensa has generally been used to clear heat and detoxify in traditional Korean and Chinese medicine. Oxaliplatin is a first-line treatment chemotherapeutic agent for advanced colorectal cancer, but it induces peripheral neuropathy as an adverse

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Neurite Outgrowth Assays

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