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OP80

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Anti-APC (Ab-7) Mouse mAb (CC-1)

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Synonym(s):
Anti-Adenomatus Polyposis Coli

biological source

mouse

Quality Level

clone

CC-1, monoclonal

form

liquid

contains

≤0.1% sodium azide

species reactivity

human, mouse, rat

manufacturer/tradename

Calbiochem®

storage condition

do not freeze

technique(s)

immunocytochemistry: suitable
immunofluorescence: suitable
immunohistochemistry (frozen sections): suitable

dilution

(Free-floating SectionsParaffin Sections)

isotype

IgG2b

shipped in

wet ice

storage temp.

2-8°C

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This Item
SAB4200594MAB3786MABC202
Anti-APC (Ab-7) Mouse mAb (CC-1)

OP80

Anti-APC (Ab-7) Mouse mAb (CC-1)

Anti-APC Antibody, clone FE9 clone FE9, from mouse

MABC202

Anti-APC Antibody, clone FE9

clone

CC-1, monoclonal

clone

polyclonal

clone

C-APC 28.9, monoclonal

clone

FE9, monoclonal

species reactivity

human, mouse, rat

species reactivity

human

species reactivity

human, mouse

species reactivity

human

storage temp.

2-8°C

storage temp.

−20°C

storage temp.

2-8°C

storage temp.

-

technique(s)

immunocytochemistry: suitable, immunohistochemistry (frozen sections): suitable, immunofluorescence: suitable

technique(s)

immunoblotting: 3-6 μg/mL using lysates of HeLa or SW620 cells., immunohistochemistry: 10-20 μg/mL using formalin-fixed, paraffin-embedded human colon or duodenum., indirect immunofluorescence: 2-4 μg/mL using Caco-2 cells.

technique(s)

immunofluorescence: suitable, immunohistochemistry: suitable, immunoprecipitation (IP): suitable, western blot: suitable

technique(s)

western blot: suitable

shipped in

wet ice

shipped in

dry ice

shipped in

ambient

shipped in

wet ice

General description

Recognizes APC in oligodendrocytes and astrocytes.

  • Antibody Target Gene Symbol: APC
  • Target Synonym: AI047805, Apc7, AU020952, AW124434, BTPS2, DP2, DP2.5, DP3, Familial adenomatous polyposis, FAP, GS, Min, RATAPC
  • Entrez Gene Name: adenomatous polyposis coli
  • Hu Entrez ID: 324 (Related Antibodies: ST1150, OP62, OP47L, OP44)
  • Mu Entrez ID: 11789
  • RatEntrez ID: 24205

Immunogen

a recombinant protein consisting of amino acids 1-226 of APC

Application

Frozen Sections (5 µg/ml) Immunofluorescence (5 µg/ml) Paraffin Sections (5 µg/ml, heat pre-treament required) Immunocytochemistry (see application references) Free Floating Sections (see application references)

Well suited for IHC and IF studies of oligodendrocytes and optic nerves due to the antibody′s staining of the cell body as opposed to the myelinated processes. Both frozen sections and formalin-fixed, paraffin-embedded brain sections can be used. Antibody should be titrated for optimal results in individual systems.

Packaging

Please refer to vial label for lot-specific concentration

Warning

Toxicity: Standard Handling

Physical form

In 50 mM sodium phosphate buffer, 0.2% gelatin.

Analysis Note

Negative control: MOPC 21 cells

Positive control: Purkinje cells or cerebellum tissue

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable


Certificates of Analysis (COA)

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J Groden et al.
Cell, 66(3), 589-600 (1991-08-09)
DNA from 61 unrelated patients with adenomatous polyposis coli (APC) was examined for mutations in three genes (DP1, SRP19, and DP2.5) located within a 100 kb region deleted in two of the patients. The intron-exon boundary sequences were defined for
L K Su et al.
Science (New York, N.Y.), 262(5140), 1734-1737 (1993-12-10)
Mutations of APC appear to initiate sporadic and inherited forms of human colorectal cancer. Although these mutations have been well characterized, little is known about the function of the APC gene product. Two cellular proteins that associate with APC were
K W Kinzler et al.
Science (New York, N.Y.), 251(4999), 1366-1370 (1991-03-15)
Recent studies have suggested the existence of a tumor suppressor gene located at chromosome region 5q21. DNA probes from this region were used to study a panel of sporadic colorectal carcinomas. One of these probes, cosmid 5.71, detected a somatically
R V Bhat et al.
Glia, 17(2), 169-174 (1996-06-01)
Mutations of the adenomatous polyposis coli (APC) tumor suppressor gene have been linked to familial polyposis, an inherited predisposition to colon cancer, and a high percentage of sporadic colon adenomas. Although this gene is best known for its role in
The APC gene product in normal and tumor cells
Smith, K.J., et al.
Proceedings of the National Academy of Sciences, 90, 2846-2850 (1993)

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