MilliporeSigma
All Photos(1)

PMEF-CFX

Sigma-Aldrich

EmbryoMax® Primary Mouse Embryonic Fibroblasts

PMEF, Strain CF1, Irradiated, Passage 3

Sign Into View Organizational & Contract Pricing

Select a Size

Synonym(s):
CF-1 MEFs, CF-1 PMEFs, Irradiated MEFs, MEF Feeder Cells, MEF Feeder Cells, CF-1 PMEFs, CF-1 MEFs, MEFs, Mouse Feeder Cells, Irradiated MEFS, Mouse Feeder Cells
eCl@ss:
32011203

biological source

mouse

Quality Level

manufacturer/tradename

Specialty Media
EmbryoMax®

technique(s)

cell culture | stem cell: suitable

input

sample type primary embryotic fibroblasts (PMEFs)
sample type induced pluripotent stem cell(s)
sample type: human embryonic stem cell(s)
sample type: mouse embryonic stem cell(s)

shipped in

liquid nitrogen

Compare Similar Items

View Full Comparison

Show Differences

1 of 4

This Item
PMEF-DR4X-MPMEF-DR4-MPMEF-HL
manufacturer/tradename

Specialty Media, EmbryoMax®

manufacturer/tradename

Specialty Media, EmbryoMax®

manufacturer/tradename

Specialty Media, EmbryoMax®

manufacturer/tradename

Specialty Media, EmbryoMax®

technique(s)

cell culture | stem cell: suitable

technique(s)

cell culture | stem cell: suitable

technique(s)

cell culture | stem cell: suitable

technique(s)

cell culture | stem cell: suitable

shipped in

liquid nitrogen

shipped in

-

shipped in

-

shipped in

liquid nitrogen

Quality Level

100

Quality Level

100

Quality Level

100

Quality Level

100

input

sample type primary embryotic fibroblasts (PMEFs)
sample type induced pluripotent stem cell(s)
sample type: human embryonic stem cell(s)
sample type: mouse embryonic stem cell(s)

input

-

input

-

input

sample type: mouse embryonic stem cell(s)
sample type primary embryotic fibroblasts (PMEFs)
sample type induced pluripotent stem cell(s)

General description

The EmbryoMax range of PMEF cells provides researchers with a convenient solution for ES cell culture by eliminating the need for time consuming feeder cell isolation and preparation. Many embryonic stem cell culture protocols necessitate the use of primary mouse embryo fibroblast (PMEF) cells. In these protocols, ES cells are typically cultured on a monolayer of PMEF feeder cells. Feeder cells perform two important roles in stem cell culture: they secrete several important growth factors into the medium, which help maintain pluripotency, and they provide a cellular matrix for ES cells to grow.
These PMEF cells were isolated from CF-1 mouse embryos at day 13 and mitotically inactivated by treatment with irradiation. The cells can be directly plated onto gelatin coated (Cat. No. ES-006-B) culture vessels. These cells are from passage 3. 5-6 x 106 cells will produce the appropriate density feeder layer on the following tissue culture vessels:

• 6 x 60mm dishes
• 2 x T75 flasks
• 2 x 6 well plates

Refer to Murine Embryonic Stem Cell Culture Manual for complete culture protocols and media formulations for PMEF cells.

Cell Line Description

Primary Mouse Embryo Fibroblasts

Application

Research Category
Stem Cell Research

Packaging

5-6x106 ea

Storage and Stability

Vials should be stored in the vapor phase of liquid nitrogen.

Legal Information

EmbryoMax is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

10 - Combustible liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Documents related to the products that you have purchased in the past have been gathered in the Document Library for your convenience.

Visit the Document Library

Difficulty Finding Your Product Or Lot/Batch Number?

Product numbers are combined with Pack Sizes/Quantity when displayed on the website (example: T1503-25G). Please make sure you enter ONLY the product number in the Product Number field (example: T1503).

Example:

T1503
Product Number
-
25G
Pack Size/Quantity

Additional examples:

705578-5MG-PW

PL860-CGA/SHF-1EA

MMYOMAG-74K-13

1000309185

enter as 1.000309185)

Having trouble? Feel free to contact Technical Service for assistance.

Lot and Batch Numbers can be found on a product's label following the words 'Lot' or 'Batch'.

Aldrich Products

  • For a lot number such as TO09019TO, enter it as 09019TO (without the first two letters 'TO').

  • For a lot number with a filling-code such as 05427ES-021, enter it as 05427ES (without the filling-code '-021').

  • For a lot number with a filling-code such as STBB0728K9, enter it as STBB0728 without the filling-code 'K9'.

Not Finding What You Are Looking For?

In some cases, a COA may not be available online. If your search was unable to find the COA you can request one.

Request COA

Megan Bowers et al.
Cell stem cell, 27(1), 98-109 (2020-05-11)
Altered neural stem/progenitor cell (NSPC) activity and neurodevelopmental defects are linked to intellectual disability. However, it remains unclear whether altered metabolism, a key regulator of NSPC activity, disrupts human neurogenesis and potentially contributes to cognitive defects. We investigated links between

Articles

Mouse Embryonic Fibroblasts (MEF Feeder Cells)

Mouse embryonic fibroblasts (MEFs) serve as a feeder layer for both mouse and human embryonic stem cells (ES cells) and induced pluripotent stem cells (iPSCs).

Pluripotent and Multipotent Stem Cells

Read types of stem cells including multipotent stem cells, Pluripotent stem cells and iPSCs and their applications in basic stem cell research, stem cell therapy and disease modelling

Protocols

Induced Pluripotent Stem Cell Reprogramming Protocols

Stem cell reprogramming protocols to generate human induced pluripotent stem cells (iPSCs) including viral and non-viral RNA based methods.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service