MilliporeSigma
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SCC038

Sigma-Aldrich

Human Adipose Mesenchymal Stem Cells

Human Adipose Mesenchymal Stem Cells (MSCs) are isolated from human adipose tissue and are cryopreserved at P2 to ensure optimal phenotype and the highest viability and plating efficiency.

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Synonym(s):
Fat Mesecnhymal Stem Cells, Human Adipose Derived MSCs, Human Adipose Stromal Stem Cell, Human Adipose Stromal Stem Cells, hADSCs
eCl@ss:
32011203

biological source

human

Quality Level

technique(s)

cell culture | mammalian: suitable
cell culture | stem cell: suitable
cell differentiation: suitable

input

sample type mesenchymal stem cell(s)

shipped in

dry ice

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14372CSCM130SCR027
Stemline® XF MSC Supplement Serum-free, liquid, sterile-filtered, suitable for cell culture

SAFC

14372C

Stemline® XF MSC Supplement

technique(s)

cell culture | mammalian: suitable, cell culture | stem cell: suitable, cell differentiation: suitable

technique(s)

cell culture | mammalian: suitable (suspension)

technique(s)

cell culture | embryo: suitable, cell culture | stem cell: suitable

technique(s)

cell culture | stem cell: suitable

shipped in

dry ice

shipped in

wet ice

shipped in

dry ice

shipped in

liquid nitrogen

Quality Level

100

Quality Level

500

Quality Level

100

Quality Level

100

input

sample type mesenchymal stem cell(s)

input

-

input

-

input

sample type mesenchymal stem cell(s)

General description

EMD Millipore’s Human Adipose MSCs are isolated from human adipose tissue and are cryopreserved at p2 to ensure optimal phenotype and the highest viability and plating efficiency. Each lot of human adipose MSCs originates from a single donor of human lipoaspirate tissue. These cells proliferate as an adherent cell monolayer and have shown a strong capacity for expansion in vitro while maintaining their potential for differentiation to multiple lineages including adipocytes, osteoblasts and chondrocytes. EMD Millipore’s Human Adipose MSCs uniformly express appropriate MSC markers including the integrin CD29, matrix receptors CD44, CD105 and stromal cell-associated markers CD73, CD90, and CD166. The cells are negative for hematopoietic lineage markers CD14, CD31, CD34, and CD45.

Components

1) 1X106 viable Human Adipose Mesenchymal Stem Cells: (Catalog No. SCC038). Store in liquid nitrogen.

Storage and Stability

Human Adipose Mesenchymal Stem Cells should be stored in liquid nitrogen. The cells can be passaged for at least three passages (or more than ten population double times) without significantly affect cell surface marker expression and differentiation potential.

Disclaimer

RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.

Storage Class Code

11 - Combustible Solids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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Xun Xu et al.
MRS communications, 11(4), 425-431 (2021-07-15)
Toll-like receptor (TLR) can trigger an immune response against virus including SARS-CoV-2. TLR expression/distribution is varying in mesenchymal stromal cells (MSCs) depending on their culture environments. Here, to explore the effect of periodic thermomechanical cues on TLRs, thermally controlled shape-memory
Yavuz Emre Arslan et al.
Biotechnology progress, 35(4), e2814-e2814 (2019-04-10)
In this study, we aimed at generating 3-dimensional (3D) decellularized bovine spinal cord extracellular matrix-based scaffolds (3D-dCBS) for neural tissue engineering applications. Within this scope, bovine spinal cord tissue pieces were homogenized in 0.1 M NaOH and this viscous mixture
Shuntaro Yamada et al.
Journal of tissue engineering, 12, 20417314211019375-20417314211019375 (2021-07-16)
The fatal determination of bone marrow mesenchymal stem/stromal cells (BMSC) is closely associated with mechano-environmental factors in addition to biochemical clues. The aim of this study was to induce osteogenesis in the absence of chemical stimuli using a custom-designed laminar

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Protocols

Protocol Guide: Expansion and Differentiation of Human Mesenchymal Stem Cells using TrueGel3D® HTS Hydrogel Plates

Learn how to culture human adipose mesenchymal stem cells (MSCs) in 3D hydrogels like the TrueGel3D® Hydrogel Plate using this step-by-step protocol for high-throughput screening cell culture applications.

Mesenchymal Stem Cell Culture Protocols

Information about mesenchyme, specifically mesenchymal stem cell procotols. Step-by-step cell culture protocols for mesenchymal stem cell (MSC) isolation, expansion and differentiation.

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