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SCC116

Sigma-Aldrich

GT1-7 Mouse Hypothalamic GnRH Neuronal Cell Line

GT1-7 cells can be used as an in vitro model of GnRH-secreting neurons of the hypothalamus.

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Synonym(s):
GT1-7 hypothalamic cell line, GT1-7 neural cell line
eCl@ss:
32011203

biological source

mouse

Quality Level

technique(s)

cell based assay: suitable
cell culture | mammalian: suitable

shipped in

ambient

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SCC134SCC065SCC129
technique(s)

cell based assay: suitable, cell culture | mammalian: suitable

technique(s)

cell based assay: suitable

technique(s)

cell based assay: suitable, cell culture | mammalian: suitable

technique(s)

cell based assay: suitable

shipped in

ambient

shipped in

ambient

shipped in

-

shipped in

dry ice

Quality Level

100

Quality Level

100

Quality Level

100

Quality Level

100

General description

GT1-7 is an immortalized mature mouse hypothalamic GnRH neuronal cell line. Immortalized GnRH neurons were generated by introducing a transgene containing the promotor region of the GnRH gene coupled to the coding region of the SV40 T-antigen oncogene into transgenic mice1. The resulting anterior hypothalamic tumors were removed from one of the mice and the cells dissociated and cloned. GT1-7 is a clonal line of mature differentiated GnRH neurons that exhibit high levels of Gnrh1 mRNA and secrete GnRH in response to depolarization.
Gonadotrophin-releasing hormone (GnRH) is a neuropeptide that is synthesized and released from GnRH neurons within the hypothalamus and is required for normal reproductive development and function. The scarcity and scattered distribution of GnRH neurons in the rostral hypothalamus make the biological study of the cells difficult.

Cell Line Description

Neural Lineage Cells

Application

Research Category
Endocrine

Neuroscience
This product is intended for sale and sold solely for internal non-commercial research use per the terms of the “Restricted Use Agreement” as detailed in the product documentation. For information regarding any other uses, please contact licensing@emdmillipore.com.

Quality

• Each vial contains ≥ 1X106 viable cells.
• Cells tested negative for infectious diseases by a Mouse/Rat Comprehensive CLEAR panel PCR panel by Charles River Animal Diagnostic Services.
• Cells tested negative for mycoplasma contamination.

Storage and Stability

Store in liquid nitrogen. The cells can be cultured for at least 10 passages after initial thawing without significantly affecting the cell marker expression and functionality.

Storage Class Code

10 - Combustible liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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P L Mellon et al.
Neuron, 5(1), 1-10 (1990-07-01)
By genetically targeting tumorigenesis to specific hypothalamic neurons in transgenic mice using the promoter region of the gonadotropin-releasing hormone (GnRH) gene to express the SV40 T-antigen oncogene, we have produced neuronal tumors and developed clonal, differentiated, neurosecretory cell lines. These
W C Wetsel et al.
Endocrinology, 129(3), 1584-1595 (1991-09-01)
An immortalized hypothalamic neuronal cell line was recently developed by genetically targeting the expression of the simian virus-40 large T-antigen in LHRH neurons. These GT1 cells were subcloned to GT1-1, GT1-3, and GT1-7 cells, and they have been shown to
D B Whyte et al.
Molecular endocrinology (Baltimore, Md.), 9(4), 467-477 (1995-04-01)
The molecular mechanisms specifying gene expression in individual neurons of the mammalian central nervous system have been difficult to study due to the cellular complexity of the brain and the absence of cultured model systems representing differentiated central nervous system
Z Liposits et al.
Endocrinology, 129(3), 1575-1583 (1991-09-01)
An immortalized LHRH cell line has recently been developed by genetically targeting these neurons for tumorigenesis. One of the subclones, the GT1-7 cells, was characterized at both the light and electron microscopic levels to study the cellular and subcellular organization
W C Wetsel et al.
Proceedings of the National Academy of Sciences of the United States of America, 89(9), 4149-4153 (1992-05-01)
Mammalian reproduction is dependent upon intermittent delivery of luteinizing hormone-releasing hormone (LHRH) to the anterior pituitary. This mode of secretion is required to sensitize maximally the gonadotrophs to LHRH stimulation and to regulate gonadotropin gene expression. While LHRH secretion is

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