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SCC117

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Histone H2B-GFP expressing HeLa Cell Line

The Histone H2B-GFP expressing HeLa cell line is a useful cell model for imaging and analysis of chromosome dynamics.

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Synonym(s):
Histone H2B
eCl@ss:
32011203

biological source

human

Quality Level

technique(s)

cell based assay: suitable
cell culture | mammalian: suitable

shipped in

ambient

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SCC293ABE1464SCC112
technique(s)

cell based assay: suitable, cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

inhibition assay: suitable (peptide), western blot: suitable

technique(s)

-

shipped in

ambient

shipped in

dry ice

shipped in

-

shipped in

ambient

Quality Level

100

Quality Level

-

Quality Level

-

Quality Level

100

General description

Chromatin, the higher order structure of DNA, proteins and RNA, constitutes the majority of the nucleus of eukaryotic cells. Changes in chromatin structure are the essence of many essential nuclear processes, including transcription, meiosis, mitosis, and apoptosis. The nucleosome represents the primary building block of chromatin and it comprises an octomer of four core histone proteins (H2A, H2B, H3 and H4). HeLa cells expressing green fluorescent protein fused histone H2B (H2B-GFP) have been widely used to visualize the dynamics of chromosomal architecture in living cells during various processes. In addition to studying normal mitosis, histone H2B-GFP has been utilized for imaging the distinctive clustering behavior of double minute chromosomes (DMs) in cancer cells during mitosis, which contributes to their asymmetric distribution to daughter cells. Monitoring histone H2B-GFP also permits continuous analysis of chromosomal degradation during apoptosis.

Cell Line Description

Cancer Cells

Application

Research Category
Apoptosis & Cancer

Epigenetics & Nuclear Function
Research Sub Category
Cell Cycle, DNA Replication & Repair

Chromatin Biology

Histones
This product is intended for sale and sold solely for internal non-commercial research use per the terms of the “Restricted Use Agreement” as detailed in the product documentation. For information regarding any other uses, please contact licensing@emdmillipore.com.

Quality

• Each vial contains ≥ 1X106 viable cells.
• Cells are tested by PCR and are negative for HPV-16, HPV-18, Hepatitis A, B, C and HIV-1 & 2 viruses.
• Cells are negative for mycoplasma contamination.
• Each lot of cells are genotyped by STR analysis to verify the unique identity of the cell line.

Storage and Stability

Histone H2B-GFP expressing HeLa Cell Line should be stored in liquid nitrogen. The cells can be cultured for at least 10 passages after initial thawing without significantly affecting the cell marker expression and functionality.

Disclaimer

RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.

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Danger

hcodes

Hazard Classifications

Acute Tox. 3 Oral

Storage Class

6.1C - Combustible, acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

wgk_germany

WGK 2


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J L Workman et al.
Annual review of biochemistry, 67, 545-579 (1998-10-06)
The nucleosome, which is the primary building block of chromatin, is not a static structure: It can adopt alternative conformations. Changes in solution conditions or changes in histone acetylation state cause nucleosomes and nucleosomal arrays to behave with altered biophysical
Athea Vichas et al.
Nature communications, 12(1), 4789-4789 (2021-08-11)
CRISPR-based cancer dependency maps are accelerating advances in cancer precision medicine, but adequate functional maps are limited to the most common oncogenes. To identify opportunities for therapeutic intervention in other rarer subsets of cancer, we investigate the oncogene-specific dependencies conferred

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