SCM139
EZ-LiFT Stem Cell Passaging Reagent
Enzyme-free pluripotent stem cell dissociation solution, 100mL
Synonym(s):
Passaging Reagent
About This Item
Recommended Products
form
liquid
technique(s)
cell culture | stem cell: suitable
General description
EZ-LiFT Stem Cell Passaging Reagent is a proprietary enzyme-free and chemically defined stem cell dissociation reagent that selectively passages only undifferentiated pluripotent stem cells. The reagent eliminates the need for manual removal of differentiated cells and produces high cell viability without the need for ROCKi. By nature of its selectivity for undifferentiated pluripotent stem cells, EZ-LiFT can be used to rescue highly differentiated hES/iPS cultures, thus enabling the recovery of precious samples and time.
EZ-LiFT does not suffer from the drawbacks of other enzyme-based dissociation reagents such as Dispase, Collagenase IV and Accutase. The disadvantages of enzyme-based reagents are that they still require manual removal of differentiated cells, suffer from lot-to-lot variability and may carry the risk for karyotypic abnormalities.
Features and Benefits
ā¢ Enzyme-free and chemically defined formulation: Produces consistent results.
ā¢ Enriches for pluripotent stem cell populations: No manual clean-up required.
ā¢ Rescues highly differentiated hES/iPS culture: Saves precious samples and time.
ā¢ Gentle on cells with high cell survival rates: ROCKi is optional, but not required.
Application
Cell Culture
Stem Cell Research
Quality
Sterility Tested: Pass
Osmolarity: 340-370 mOsm
Storage and Stability
Disclaimer
Storage Class
12 - Non Combustible Liquids
wgk_germany
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
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Articles
Read types of stem cells including multipotent stem cells, Pluripotent stem cells and iPSCs and their applications in basic stem cell research, stem cell therapy and disease modelling
Protocols
Human ips cell passaging protocol using EZ-LiFTā¢ Reagent, an enzyme-free and chemically defined pluripotent stem cell dissociation reagent.
Step-by-step stem cell culture protocols for human induced pluripotent stem cells (iPSCs) including ips cell thawing, expanding, freezing and characterizing.
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