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SCM151

Sigma-Aldrich

PLTGold® Human Platelet Lysate

Heparin-free Human Platelet Lysate (hPL) for Mesenchymal Stem Cell Culture. 100ml.

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Synonym(s):
hPL, hPL Human Platelet Lysate Supplement
eCl@ss:
32160801

biological source

human

form

liquid

technique(s)

cell culture | stem cell: suitable

input

sample type mesenchymal stem cell(s)

shipped in

ambient

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This Item
CC130H3667SCM05
Human Serum Heat Inactivated, from human male AB plasma, USA origin, sterile-filtered

Sigma-Aldrich

H3667

Human Serum

form

liquid

form

liquid

form

-

form

liquid

technique(s)

cell culture | stem cell: suitable

technique(s)

cell attachment: suitable, cell culture | mammalian: suitable, cell culture | stem cell: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable, cell culture | stem cell: suitable

shipped in

ambient

shipped in

dry ice

shipped in

dry ice

shipped in

-

input

sample type mesenchymal stem cell(s)

input

sample type: human embryonic stem cell(s)

input

-

input

-

General description

PLTGold Human Platelet Lysate is a Xeno-Free cell culture supplement that is a superior alternative to fetal bovine serum (FBS) for use in human mesenchymal stem cell cultures. PLTGold is an unfractionated product derived from human platelets that does not require the addition of heparin. It remains clot-free with performance equivalent to the original PLTMax platelet lysate. Optimal growth of MSCs can be achieved with 5% PLTGold. PLTGold been used to effectively to grow mesenchymal stem cells derived from other species including mouse, rabbit and porcine.

PLTGold Human Platelet Lysate is derived from normal human donor platelets collected at U.S. blood centers. Multiple donors units are pooled in large batch sizes and manufactured to produce a consistent product. Human Platelet Lysate is for research use only (RUO) and not intended for human or animal diagnostic or therapeutic use.

Application

Instructions for Use

1.Cell seeding should be performed following the general guidelines for the specific cell type. For Mesenchymal Stem Cells (MSCs), cells are typically plated at approximately 2x10^3 – 5x10^3 cells per cm2.
2. For MSCs, PLTGold can be used at 5% vol/vol in a typical cell culture medium such as DMEM (Sigma D5671) or α-MEM (Sigma M8042). If the basic media doesn’t contain Glutamine, a source of L-Glutamine (Sigma G7513) will need to be added to the media at a final concentration of 2mM. For other types of cells, the concentration of PLTGold will need to be titrated according to the application (a titration from 2% vol/vol to 10% vol/vol is recommended to establish the percentage of PLTGold needed for the cell type to use).
3. Do not allow primary MSC confluence to exceed 70-80%.
Research Category
Stem Cell Research
Research Sub Category
Mesenchymal Stem Cells

Quality

All human derived materials are obtained from government inspected facilities and are of US origin. Each donor unit was approved for human use and has been tested for infectous diseases including HIV-1, HIV-2, HIV-NAT, HCV, HBsAg, and RPR for Syphilis.

Each lot is extensively tested for the following parameters:
Sterility Testing: Negative for growth
pH: 6.8-7.8
Total protein: 4.0-6.5 g/dl
Osmolality: 250-330 mOsm/kg
Endotoxin USP <8>: <10 EU/mL
Mycoplasma: Not detected
Cell Growth: Pass

Storage and Stability

Store at -80°C upon receipt. Thaw at room temperature or in a water bath. After thawing, aliquot and store at -20°C or colder. Long term storage at -80°C is recommended. Multiple freeze/thaw cycles are not recommended.

Legal Information

PLTGold is a registered trademark of Mill Creek Life Sciences, LLC

Disclaimer

RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.
RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

10 - Combustible liquids

WGK

nwg

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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