SCM151
PLTGold® Human Platelet Lysate
Heparin-free Human Platelet Lysate (hPL) for Mesenchymal Stem Cell Culture. 100ml.
Synonym(s):
hPL, hPL Human Platelet Lysate Supplement
About This Item
Recommended Products
biological source
human
form
liquid
technique(s)
cell culture | stem cell: suitable
input
sample type mesenchymal stem cell(s)
shipped in
ambient
Related Categories
General description
PLTGold Human Platelet Lysate is derived from normal human donor platelets collected at U.S. blood centers. Multiple donors units are pooled in large batch sizes and manufactured to produce a consistent product. Human Platelet Lysate is for research use only (RUO) and not intended for human or animal diagnostic or therapeutic use.
Application
1.Cell seeding should be performed following the general guidelines for the specific cell type. For Mesenchymal Stem Cells (MSCs), cells are typically plated at approximately 2x10^3 – 5x10^3 cells per cm2.
2. For MSCs, PLTGold can be used at 5% vol/vol in a typical cell culture medium such as DMEM (Sigma D5671) or α-MEM (Sigma M8042). If the basic media doesn’t contain Glutamine, a source of L-Glutamine (Sigma G7513) will need to be added to the media at a final concentration of 2mM. For other types of cells, the concentration of PLTGold will need to be titrated according to the application (a titration from 2% vol/vol to 10% vol/vol is recommended to establish the percentage of PLTGold needed for the cell type to use).
3. Do not allow primary MSC confluence to exceed 70-80%.
Stem Cell Research
Mesenchymal Stem Cells
Quality
Each lot is extensively tested for the following parameters:
Sterility Testing: Negative for growth
pH: 6.8-7.8
Total protein: 4.0-6.5 g/dl
Osmolality: 250-330 mOsm/kg
Endotoxin USP <8>: <10 EU/mL
Mycoplasma: Not detected
Cell Growth: Pass
Storage and Stability
Legal Information
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
wgk_germany
nwg
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Articles
Read types of stem cells including multipotent stem cells, Pluripotent stem cells and iPSCs and their applications in basic stem cell research, stem cell therapy and disease modelling
Development of a novel serum-free and xeno-free human mesenchymal stem cell (MSC) osteocyte differentiation media.
PLTMax® Human Platelet Lysate (hPL) is a superior serum-free and xeno-free media supplement alternative to fetal bovine serum (FBS) for human mesenchymal stem cell (MSC) cultures.
Frequently asked questions about mesenchymal stem cells including MSC derivation, expansion, differentiation and allogenic stem cell therapy.
Protocols
Information about mesenchyme, specifically mesenchymal stem cell procotols. Step-by-step cell culture protocols for mesenchymal stem cell (MSC) isolation, expansion and differentiation.
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
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