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SCR001

Sigma-Aldrich

ES Cell Characterization Kit

The Embryonic Stem Cell Characterization Kit phenotypically assesses the differentiation status of ES cells by measuring their AP activity, cell-surface stage-specific antigens (SSEA-1, SSEA-4) as well as expression of TRA-1-60, TRA-1-81 antigens.

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eCl@ss:
32161000

Quality Level

species reactivity

human, mouse, rat

manufacturer/tradename

Chemicon®

technique(s)

cell culture | stem cell: suitable
immunocytochemistry: suitable

input

sample type: human embryonic stem cell(s)
sample type: mouse embryonic stem cell(s)
sample type induced pluripotent stem cell(s)

shipped in

wet ice

General description

The ES Cell Characterization Kit consists of two components used for alkaline phosphatase activity determination as well as four ES cell-specific antibodies required to perform 100 tests (including controls).

INTRODUCTION

Stem cells have become the subject of extensive investigation recently, partly due to their therapeutic potential and because they raise several fundamental issues concerning the regulation of proliferation and differentiation.

Embryonic stem (ES) cells are derived from totipotent cells of the early mammalian embryo and are capable of unlimited, undifferentiated proliferation in vitro [Evans and Kaufman, 1981; Martin, 1981; Morrison, 1997]. Undifferentiated mouse ES cells can be maintained for an extensive period of time in media containing the cytokine, leukemia-inhibitory factor (LIF) or CHEMICON′s proprietary ES cell culture reagent, ESGRO® [Smith, 1988; Williams, 1988]. However, upon removal of LIF from the culture medium, in vitro, the mouse ES cells start to differentiate into cells derived from all three germ layers. In contrast, human ES cultures require mouse fibroblasts as feeder cells and cannot be maintained with LIF for self-renewal [Thomson and Marshall, 1998; Shamblott, 1988].

The undifferentiated state of the embryonic stem cell is characterized by a high level of expression of alkaline phosphatase (AP) [Pease, 1990] and the stem cell transcription factor, Oct-4. Nevertheless, these ES cells also exhibit marked differences from their murine counterparts in regards to their expression of stage-specific embryonic antigen (SSEA), that typify undifferentiated human ES and embryonic carcinoma (EC) cells.

SSEA-1, a carbohydrate antigen, is a fucosylated derivative of type 2 polylactosamine and appears during late cleavage stages of mouse embryos. It is strongly expressed by undifferentiated, murine ES cells [Solter and Knowles, 1978; Gooi, 1981]. Upon differentiation, murine ES cells are characterized by the loss of SSEA-1 expression and may be accompanied, in some instances, by the appearance of SSEA-3 and SSEA-4 [Solter, 1979]. In contrast, human ES and EC cells typically express SSEA-3 and SSEA-4 but not SSEA-1, while their differentiation is characterized by downregulation of SSEA-3 and SSEA-4 and an upregulation of SSEA-1 [Andrews, 1984; Fenderson and Andrews; 1987]. Undifferentiated, human ES cells also express the keratin sulphate-associated antigens, TRA-1-60 and TRA-1-81 [Andrews and Banting, 1984].

CHEMICON′s ES Cell Characterization Kit (Catalog number SCR001) is a specific and sensitive tool for the phenotypic assessment of the differentiation status of ES cells by measuring their AP activity, cell-surface stage-specific antigens (SSEA-1, SSEA-4) as well as expression of TRA-1-60, TRA-1-81 antigens.

Application

Research Category
Stem Cell Research
The Embryonic Stem Cell Characterization Kit phenotypically assesses the differentiation status of ES cells by measuring their AP activity, cell-surface stage-specific antigens (SSEA-1, SSEA-4) as well as expression of TRA-1-60, TRA-1-81 antigens.

Components

Fast Red Violet solution (0.8g/L stock) (Part No. 90239). One 15mL bottle.

Napthol AS-BI phosphate solution (4mg/mL) in AMPD buffer (2mol/L), pH 9.5 (Part No. 90234). One 15mL bottle.

MS X SSEA-1, IgM, clone MC-480 (Part No. 90230). One vial containing 100μl of 1mg/mL monoclonal antibody.

MS X SSEA-4, IgG, clone MC-813-70 (Part No. 90231). One vial containing 100μl of 1mg/mL monoclonal antibody.

MS X TRA-1-60, IgM, clone TRA-1-60 (Part No. 90232). One vial containing 100μl of 1mg/mL monoclonal antibody

MS X TRA-1-81, IgM, clone TRA-1-81 (Part No: 90233). One vial containing 100μl of 1mg/mL monoclonal antibody.

Storage and Stability

When stored at 2-8º C, the kit components are stable up to the expiration date. Do not freeze or expose to elevated temperatures. Discard any remaining reagents after the expiration date.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
ESGRO is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Hazard Classifications

Carc. 2 - Eye Irrit. 2 - Skin Irrit. 2

Storage Class

10 - Combustible liquids

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WGK 3


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Protocols

Step-by-step stem cell culture protocols for human induced pluripotent stem cells (iPSCs) including ips cell thawing, expanding, freezing and characterizing.

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