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SCR030

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Human Embryonic Germ Layer Characterization Kit

CHEMICON′s Human Embryonic Germ Layer Characterization Kit allows researchers to assess the differentiation potential of their Human Embryonic Stem Cell (HESC) to form derivatives of all three embryonic germ layers.

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eCl@ss:
32161000

Quality Level

species reactivity

human

manufacturer/tradename

Chemicon®

technique(s)

cell culture | stem cell: suitable
immunocytochemistry: suitable

input

sample type induced pluripotent stem cell(s)
sample type: human embryonic stem cell(s)

shipped in

wet ice

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Quality Level

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Quality Level

100

Quality Level

100

input

sample type induced pluripotent stem cell(s)
sample type: human embryonic stem cell(s)

input

sample type: human embryonic stem cell(s)
sample type induced pluripotent stem cell(s)

input

sample type neural stem cell(s)

input

sample type neural stem cell(s)

technique(s)

cell culture | stem cell: suitable, immunocytochemistry: suitable

technique(s)

cell culture | stem cell: suitable, immunocytochemistry: suitable

technique(s)

cell culture | stem cell: suitable, immunocytochemistry: suitable

technique(s)

cell culture | stem cell: suitable, immunocytochemistry: suitable

shipped in

wet ice

shipped in

wet ice

shipped in

dry ice

shipped in

dry ice

General description

Human embryonic stem cells (HESC) are pluripotent stem cells that are capable of self-renewal and of differentiation in vitro and in vivo into a wide variety of cell types, including derivatives of all three embryonic germ layers. These primary germ layers will eventually give rise to all the tissues and organs of the body.



Mesoderm: bone, cartilage, muscle, blood and vascular system

Endoderm: gastrointestinal tract, respiratory tract and endocrine glands (including liver and pancreas)

Ectoderm: nervous system, skin



CHEMICON®′s Human Embryonic Germ Layer Characterization Kit (SCR030) allows researchers to assess the differentiation potential of their HESC to form derivatives of all three embryonic germ layers. Included in the kit are antibodies that are routinely used by stem cell researchers to identify the lineage fates of HESC following either spontaneous or directed differentiation.



Mesoderm: Smooth Muscle Actin (CBL171)

Cardiac Troponin I (MAB1691-50UG)



Endoderm: Alpha Fetoprotein (2004189)



Ectoderm: Nestin (ABD69)

MAP-2 (MAB3418-50UG)

Application

CHEMICON′s Human Embryonic Germ Layer Characterization Kit allows researchers to assess the differentiation potential of their Human Embryonic Stem Cell (HESC) to form derivatives of all three embryonic germ layers.
Research Category
Stem Cell Research

Components

50 μL of Alpha Fetoprotein mouse monoclonal 2004189

50 μg of Smooth Muscle Actin mouse monoclonal CBL171

50 μL of Nestin rabbit polyclonal ABD69

50 μg of Cardiac Troponin I mouse monoclonal MAB1691-50UG

50 μg of MAP-2 mouse monoclonal MAB3418-50UG

Storage and Stability

Maintain components according to the individual data sheets. Antibodies to alpha fetoprotein (2004189) and nestin (ABD69) are to be stored at -20°C (SCR030 - Part 1). Antibodies to smooth muscle actin (CBL171), cardiac troponin I (MAB1691-50UG) and MAP-2 (MAB3418-50UG) are to be stored at 2 - 8°C (SCR030 - Part 2).

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Hazard Classifications

Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Aquatic Chronic 3

Storage Class

10 - Combustible liquids

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WGK 3


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Julie Brault et al.
BioResearch open access, 3(6), 311-326 (2014-12-04)
Chronic granulomatous disease (CGD) is an inherited orphan disorder caused by mutations in one of the five genes encoding reduced nicotinamide-adenine-dinucleotide-phosphate oxidase subunits, which subsequently lead to impairment in the production of microbicidal reactive oxygen species (ROS). In order to
Murali Krishna Mamidi et al.
Journal of biomolecular screening, 15(6), 630-643 (2010-06-10)
Techniques to evaluate gene expression profiling, including real-time quantitative PCR, TaqMan low-density arrays, and sufficiently sensitive cDNA microarrays, are efficient methods for monitoring human embryonic stem cell (hESC) cultures. However, most of these high-throughput tests have a limited use due

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