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SCR544

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Human STEMCCA Constitutive Polycistronic (OKSM) Lentivirus Reprogramming Kit

The Human STEMCCA Constitutive Polycistronic Lentivirus Kit contains high titer polycistronic lentivirus & Polybrene transfection reagent that have been validated for the generation of human iPS cells from human foreskin fibroblasts.

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eCl@ss:
32161000

Quality Level

manufacturer/tradename

STEMCCA

technique(s)

cell culture | stem cell: suitable

input

sample type induced pluripotent stem cell(s)

shipped in

dry ice

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manufacturer/tradename

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manufacturer/tradename

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technique(s)

cell culture | stem cell: suitable

technique(s)

cell culture | stem cell: suitable

technique(s)

cell culture | stem cell: suitable, immunocytochemistry: suitable

technique(s)

cell culture | stem cell: suitable, immunocytochemistry: suitable

shipped in

dry ice

shipped in

dry ice

shipped in

wet ice

shipped in

wet ice

input

sample type induced pluripotent stem cell(s)

input

sample type induced pluripotent stem cell(s)
sample type: mouse embryonic stem cell(s)

input

sample type induced pluripotent stem cell(s)
sample type: human embryonic stem cell(s)

input

sample type: human embryonic stem cell(s)
sample type induced pluripotent stem cell(s)

General description

The Human STEMCCA Constitutive Polycistronic (OKSM) Lentivirus Kit contains high titer polycistronic (OKSM) lentivirus and Polybrene transfection reagent that have been validated for the generation of human induced pluripotent stem cells from human foreskin fibroblasts (HFFs). The STEMCCA vector is comprised of the transcription factors human Oct-4, Klf4, SOX-2, and c-Myc (OKSM), separated by the self-cleaving 2A peptide and IRES sequences (Sommer CA, 2009). The use of a single polycistronic lentiviral vector significantly improves reprogramming efficiencies and reduces the number of viral integrations.

Components

1. Human STEMCCA Constitutive (OKSM) Lentivirus: (Part number SCR544-1) Two (2) vials of EF1α-hSTEMCCA (OKSM) Lentivirus (Part number CS204502). Each vial contains 15 µL of high titer lentivirus.

2. Polybrene Transfection Reagent: (Part number TR-1003-50UL) One (1) vial containing 50 µL of 10 mg/mL stock.

Quality

Tested to confirm the generation of iPS cells from p6 human foreskin fibroblasts. Other cell types have not been tested and thus similar results can not be guaranteed.

Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable


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Frank Griscelli et al.
Oncotarget, 10(28), 2693-2708 (2019-05-21)
Recent development of cell reprogramming technologies brought a major hope for future cell therapy applications by the use of these cells or their derivatives. For this purpose, one of the major requirements is the absence of genomic alterations generating a
Yang Yu et al.
Scientific reports, 5, 10114-10114 (2015-05-13)
Human pluripotent stem cells, including cloned embryonic and induced pluripotent stem cells, offer a limitless cellular source for regenerative medicine. However, their derivation efficiency is limited, and a large proportion of cells are arrested during reprogramming. In the current study
Jun-Quan Li et al.
Molecular medicine reports, 9(3), 837-842 (2014-01-09)
Myocardial infarction (MI) is an increasing medical problem; however, its pathogenesis has yet to be elucidated and more effective treatment strategies are required. Induced pluripotent stem cells (iPSCs) were recently successfully generated using human somatic cells transfected with four transcription
Areechun Sotthibundhu et al.
Stem cell research & therapy, 7(1), 166-166 (2016-11-17)
Cellular reprogramming is a stressful process, which requires cells to engulf somatic features and produce and maintain stemness machineries. Autophagy is a process to degrade unwanted proteins and is required for the derivation of induced pluripotent stem cells (iPSCs). However
Martin Madill et al.
Molecular brain, 10(1), 22-22 (2017-06-15)
Amyotrophic lateral sclerosis, a devastating neurodegenerative disease, is characterized by the progressive loss of motor neurons and the accumulation of misfolded protein aggregates. The latter suggests impaired proteostasis may be a key factor in disease pathogenesis, though the underlying mechanisms

Articles

Fibroblast growth factors (FGFs) are secreted glycoproteins that regulate several fundamental developmental pathways and help regulate mesoderm and ectoderm patterning in the early embryonic development.

Protocols

Stem cell reprogramming protocols to generate human induced pluripotent stem cells (iPSCs) including viral and non-viral RNA based methods.

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