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SE1M003M00

Millipore

Millipore® Steriflip® Vacuum Tube Top Filter

pore size 0.45 μm, PVDF membrane, funnel capacity 50 mL, pack of 25 ea

Synonym(s):

Steriflip®-HV Sterile Centrifuge Tube Top Filter Unit, tube top sterile filter unit, tube top vacuum filter, tube top vacuum sterile filtering unit

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About This Item

UNSPSC Code:
41104914
eCl@ss:
32031602
NACRES:
NB.22

material

PVDF membrane
acrylic housing
polypropylene

Quality Level

sterility

sterile

product line

Steriflip®

feature

holdup volume 0.6 mL
hydrophilic

packaging

pack of 25 ea

parameter

45 °C max. temp.

technique(s)

sterile filtration: suitable

H

138 mm

capacity

50 mL

diam.

6.4 cm

filter diam.

4 cm

filtration area

7 cm2

funnel capacity

50 mL

matrix

Durapore®

pore size

0.45 μm pore size

fitting

double lead thread inlet (with vacuum port)
double lead thread outlet for 50 mL centrifuge tube

shipped in

ambient

Application

For Research Use Only

Steriflip® filters are designed to be used for sterile vacuum filtration of aqueous solutions such as tissue culture media and biological fluids.

  • Tissue culture media +/- additives
  • Buffers
  • Biological solutions
Steriflip® filter has been used for lentivirus production.

Features and Benefits

  • Eliminates messy sample transfer via a simple vacuum-assisted, closed system
  • Can isolate cells from cellular debris using any of the various pore-sized nylon net filters
  • Ideal for sterilizing small batches of culture media
  • Attaches to any standard 50 mL centrifuge tube, eliminating unnecessary materials, time and risk of spillage

Legal Information

Durapore is a registered trademark of Merck KGaA, Darmstadt, Germany
Millipore is a registered trademark of Merck KGaA, Darmstadt, Germany
Steriflip is a registered trademark of Merck KGaA, Darmstadt, Germany

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Jiye Liu et al.
Science advances, 7(23) (2021-06-06)
Immunomodulatory drugs (IMiDs) have markedly improved patient outcome in multiple myeloma (MM); however, resistance to IMiDs commonly underlies relapse of disease. Here, we identify that tumor necrosis factor (TNF) receptor-associated factor 2 (TRAF2) knockdown (KD)/knockout (KO) in MM cells mediates
Laura M Chambers et al.
PloS one, 15(4), e0228511-e0228511 (2020-04-30)
To evaluate intraperitoneal (IP) tumor engraftment, metastasis and growth in a pre-clinical murine epithelial ovarian cancer (EOC) model using both transabdominal ultrasound (TAUS) and bioluminescence in vivo imaging system (IVIS). Ten female C57Bl/6J mice at six weeks of age were
Mateusz Legut et al.
Cell reports, 30(9), 2859-2868 (2020-03-05)
A key limitation of the widely used CRISPR enzyme S. pyogenes Cas9 is the strict requirement of an NGG protospacer-adjacent motif (PAM) at the target site. This constraint can be limiting for genome editing applications that require precise Cas9 positioning. Recently
Xiaojun Lian et al.
Nature protocols, 8(1), 162-175 (2012-12-22)
The protocol described here efficiently directs human pluripotent stem cells (hPSCs) to functional cardiomyocytes in a completely defined, growth factor- and serum-free system by temporal modulation of regulators of canonical Wnt signaling. Appropriate temporal application of a glycogen synthase kinase
Jiye Liu et al.
Leukemia, 33(1), 171-180 (2018-07-22)
Immunomodulatory drugs (IMiDs) including lenalidomide and pomalidomide bind cereblon (CRBN) and activate the CRL4CRBN ubiquitin ligase to trigger proteasomal degradation of the essential transcription factors IKZF1 and IKZF3 and multiple myeloma (MM) cytotoxicity. We have shown that CRBN is also

Protocols

Detergents are frequently used to solubilize proteins and nucleic acids during purification, but the presence of detergents may interfere with downstream analyses.

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