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10280879001

Roche

5-Bromo-2′-deoxyuridine

>97%, crystalline solid, pkg of 1 g

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Synonym(s):
5-Bromo-2′-deoxyuridine, brdu, 5-BrdU, 5-Bromo-1-(2-deoxy-β-D-ribofuranosyl)uracil, 5-Bromouracil deoxyriboside, BUdR
Empirical Formula (Hill Notation):
C9H11BrN2O5
CAS Number:
Molecular Weight:
307.10
Beilstein/REAXYS Number:
30395
MDL number:
PubChem Substance ID:

Quality Level

assay

>97%

form

crystalline solid

mol wt

307.1

packaging

pkg of 1 g

manufacturer/tradename

Roche

mp

191-194 °C (dec.) (lit.)

shipped in

wet ice

storage temp.

2-8°C

SMILES string

OC[C@H]1O[C@H](C[C@@H]1O)N2C=C(Br)C(=O)NC2=O

InChI

1S/C9H11BrN2O5/c10-4-2-12(9(16)11-8(4)15)7-1-5(14)6(3-13)17-7/h2,5-7,13-14H,1,3H2,(H,11,15,16)/t5-,6+,7+/m0/s1

InChI key

WOVKYSAHUYNSMH-RRKCRQDMSA-N

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This Item
B5002B9285B0631
5-Bromo-2′-deoxyuridine >97%, crystalline solid, pkg of 1 g

10280879001

5-Bromo-2′-deoxyuridine

5-Bromo-2′-deoxyuridine ≥99% (HPLC)

B5002

5-Bromo-2′-deoxyuridine

5-Bromo-2′-deoxyuridine BioUltra, ≥99%

B9285

5-Bromo-2′-deoxyuridine

Quality Level

100

Quality Level

200

Quality Level

-

Quality Level

-

form

crystalline solid

form

powder

form

powder

form

powder

assay

>97%

assay

≥99% (HPLC)

assay

≥99%

assay

≥90%

storage temp.

2-8°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

packaging

pkg of 1 g

packaging

-

packaging

-

packaging

-

General description

It is conventionally used as a marker for S-phase synthesis of DNA.

Application

5-Bromo-2′-deoxyuridine has been used to label cells in the S-phase of the cell cycle and primary hepatocytes during cell proliferation studies.
Immunochemically detectable nucleotide that can be incorporated into DNA instead of thymidine. Used for in vivo studies of DNA synthesis and for the detection of mutagenic substances, according to the sister chromatid exchange (SCE) method.

Biochem/physiol Actions

Thymidine analog used as a mutagen in genetic research. Selectively incorporated into cellular DNA during S-phase.

Quality

Purity: 97% (from N), chromatographically homogeneous

Preparation Note

Working concentration: According to literature for cell culture, a final concentration of 10 μmol/l has been used.
According to literature, 5 to 50 mg/kg body weight mice have been used for the detection of cell proliferation.

Preparation of Working Solution
Corresponding to the BrdU-labeling reagent which is included in the Roche Cell Proliferation ELISA, BrdU kits and in the Roche 5-Bromo-2′-deoxy-uridine Labeling and Detection Kits, prepare the BrdU working solution by dissolving the substance in PBS to a 10 mM stock solution (MW of BrdU = 307.1 D). For the in vivo use of BrdU dissolve in PBS; for the in vitro use of BrdU, dissolve in double-distilled water at the same 10 mM concentration.
Storage conditions (working solution): -15 to -25 °C

Other Notes

For life science research only. Not for use in diagnostic procedures.

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Effects of Wnt5a protein on proliferation and apoptosis in JAR choriocarcinoma cells
Peng S, et al.
Molecular Medicine Reports, 4(1), 99-104 (2011)
K M Connolly et al.
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, 41(1), 1-6 (1993-01-01)
Proliferating cell nuclear antigen (PCNA) was evaluated as a marker of cell proliferation in formalin-fixed rat liver tissue through a comparative study with the thymidine analogue 5-bromo-2'-deoxyuridine (BrdU). The comparison was conducted through the introduction of a dual immunohistochemical procedure
Loss of Integrin $\alpha$v$\beta$8 in Murine Hepatocytes Accelerates Liver Regeneration
Greenhalgh SN, et al.
The American Journal of Pathology, 189(2), 258-271 (2019)
Yukinobu Goto et al.
American journal of physiology. Cell physiology, 285(2), C253-C259 (2003-04-04)
The present study was designed to develop methods to study the production and release of monocytes from the bone marrow using the thymidine analog 5'-bromo-2'-deoxyuridine (BrdU). Dividing monocytes in bone marrow were labeled with BrdU (MOBrdU), and their release into
Hwan-Ki Kim et al.
Experimental neurobiology, 29(6), 417-424 (2020-12-08)
The myelination of axons in the vertebrate nervous system through oligodendrocytes promotes efficient axonal conduction, which is required for the normal function of neurons. The central nervous system (CNS) can regenerate damaged myelin sheaths through the process of remyelination, but

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