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SAB4700630

Sigma-Aldrich

Monoclonal Anti-5-bromodeoxyuridine antibody produced in mouse

clone MoBu-1, purified immunoglobulin, buffered aqueous solution

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Synonym(s):
Anti-BrdU
NACRES:
NA.41

biological source

mouse

Quality Level

conjugate

unconjugated

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

MoBu-1, monoclonal

form

buffered aqueous solution

concentration

1 mg/mL

technique(s)

flow cytometry: suitable

isotype

IgG1

shipped in

wet ice

storage temp.

2-8°C

target post-translational modification

unmodified

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This Item
B8434B2531SAB4701040
clone

MoBu-1, monoclonal

clone

BU-33, monoclonal

clone

BU-33, monoclonal

clone

Bu20a, monoclonal

biological source

mouse

biological source

mouse

biological source

mouse

biological source

mouse

conjugate

unconjugated

conjugate

unconjugated

conjugate

unconjugated

conjugate

unconjugated

Quality Level

100

Quality Level

200

Quality Level

200

Quality Level

-

form

buffered aqueous solution

form

buffered aqueous solution

form

-

form

buffered aqueous solution

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General description

The antibody MoBu-1 reacts specifically with BrdU incorporated into DNA during S-phase of a cell cycle. The antibody MoBu-1 is also useful for detecting proliferating cells by flow cytometry or immunofluorescence staining. It reacts also specifically with 5-bromouridine (BrU).

Immunogen

5-bromodeoxyuridine conjugated with hemocyanine

Application

Monoclonal Anti-5-bromodeoxyuridine antibody produced in mouse has been used in Immunocytochemistry. The reagent is designed for Flow Cytometry analysis. Suggested working dilution for Flow Cytometry is 1-2 μg/mL of sample. Indicated dilution is recommended starting point for use of this product. Working concentrations should be determined by the investigator.

Biochem/physiol Actions

5-Bromodeoxyuridine (BrdU) is an analogue of thymidine, used to measure DNA synthesis immunochemically in living cells. BrdU along with cAMP and butyrate plays a vital role in the cellular differentiation in several mammalian cell types. It also inhibits cell growth in mammalian and yeast cells by modulating the expression of particular genes involved in cell division. In the absence of DNA synthesis, brdu promotes mouse neuroblastoma C1300 cell differentiation into cells that morphologically are similar to mature neurons. BrdU activates cell senescence-like phenomenon in mammalian cells.

Features and Benefits

Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more.

Physical form

Solution in phosphate buffered saline, pH 7.4, with 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

10 - Combustible liquids

flash_point_f

Not applicable

flash_point_c

Not applicable


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Masahiro Nakano et al.
Communications biology, 4(1), 858-858 (2021-07-11)
The single-stranded, negative-sense, viral genomic RNA (vRNA) of influenza A virus is encapsidated by viral nucleoproteins (NPs) and an RNA polymerase to form a ribonucleoprotein complex (vRNP) with a helical, rod-shaped structure. The vRNP is responsible for transcription and replication
Alessio Ligabue et al.
PloS one, 7(10), e47318-e47318 (2012-10-12)
5-fluorouracil, a commonly used chemotherapeutic agent, up-regulates expression of human thymidylate synthase (hTS). Several different regulatory mechanisms have been proposed to mediate this up-regulation in distinct cell lines, but their specific contributions in a single cell line have not been
AT-hook proteins stimulate induction of senescence markers triggered by 5-bromodeoxyuridine in mammalian cells.
Satou W, et al.
Experimental Gerontology, 39(2), 173-179 (2004)
5-bromodeoxyuridine-induced differentiation of a neuroblastoma.
Schubert D and Jacob F.
Proceedings of the National Academy of Sciences of the USA, 67(1), 247-254 (1970)
Ying Zhao et al.
Cell cycle (Georgetown, Tex.), 21(19), 2027-2037 (2022-06-07)
The mammary gland of mammals possesses the specific function of synthesizing, secreting, and delivering milk. Notably, mammary epithelial cells are considered to be central to control the expansion and remodeling of mammary gland into a milk-secretory organ. And the biological

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