MilliporeSigma
All Photos(4)

Documents

B8434

Sigma-Aldrich

Anti-BrdU antibody, Mouse monoclonal

clone BU-33, purified from hybridoma cell culture

Sign Into View Organizational & Contract Pricing

Synonym(s):
Monoclonal Anti-BrdU antibody produced in mouse, Anti Brdu, Brdu Antibody, Brdu Antibody - Anti-BrdU antibody, Mouse monoclonal, Anti-Bromodeoxyuridine
MDL number:
NACRES:
NA.41

biological source

mouse

Quality Level

conjugate

unconjugated

antibody form

purified from hybridoma cell culture
purified immunoglobulin

antibody product type

primary antibodies

clone

BU-33, monoclonal

form

buffered aqueous solution

species reactivity

wide range

packaging

antibody small pack of 25 μL

concentration

~1.5 mg/mL

technique(s)

immunocytochemistry: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1-2 μg/mL using sections of intestine from mice or rats treated in vivo with BrdU

isotype

IgG1

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Looking for similar products? Visit Product Comparison Guide

Compare Similar Items

View Full Comparison

Show Differences

1 of 4

This Item
V6389MAB4072MAB3222
Sigma-Aldrich

MAB3222

Anti-BrdU Antibody, clone IIB5

biological source

mouse

biological source

mouse

biological source

mouse

biological source

mouse

clone

BU-33, monoclonal

clone

V9, monoclonal

clone

131-14871, monoclonal

clone

IIB5, monoclonal

species reactivity

wide range

species reactivity

hamster, monkey, dog, horse, bovine, rat, gerbil, chicken, pig, feline, human

species reactivity

-

species reactivity

-

conjugate

unconjugated

conjugate

unconjugated

conjugate

-

conjugate

-

Quality Level

200

Quality Level

200

Quality Level

100

Quality Level

100

Still not finding the right product?  

Give our Product Selector Tool a try.

General description

5′-Bromo-2-deoxyuridine (BrdU) is a carcinogen that may be absorbed by skin or inhalation. BrdU solutions are sensitive to light. Bromodeoxyuridine (5-Bromo-2-Deoxyuridine, BrdU) is an analogue of thymidine that is selectively incorporated into cell DNA at the S phase of the cell cycle.
Anti-BrdU antibody, Mouse monoclonal (mouse IgG1) is derived from the BU-33 hybridoma produced by the fusion of murine myeloma cells and splenocytes from BALB/c mouse immunized with bromodeoxyuridine (BrdU) conjugated to KLH.5′-Bromo-2-deoxyuridine (BrdU) staining is predominantly used to observe the multiplication of tumor cells and other tissues in vivo.

Specificity

Reacts specifically with BrdU (bromodeoxyuridine) incorporated into DNA or coupled to a protein carrier. Cross-reaction with IdU (iododeoxyuridine) is observed.

Immunogen

bromodeoxyuridine conjugated to KLH.

Application

Anti-BrdU antibody has been used in:
  • BrdU labeling
  • immunohistochemistry
  • immunostaining
  • BrdU assay
Anti-BrdU antibody, Mouse monoclonal has been used in immunostaining, as a primary antibody:
  • to detect 5-fluorouridine-labelled RNA
  • to detect 5-bromo-2′-deoxyuridine (BrdU) labelled immunoreactive cells
  • against BrdU labelled rat retinal sections

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium axide.

Storage and Stability

For continuous use, store at 2-8 °C for up to one month. For prolonged storage, freeze in working aliquots at -20 °C. Repeated freezing and thawing, or storage in “frost-free” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation. Working dilution samples should be discarded if not used within 12 hours.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Documents related to the products that you have purchased in the past have been gathered in the Document Library for your convenience.

Visit the Document Library

Difficulty Finding Your Product Or Lot/Batch Number?

Product numbers are combined with Pack Sizes/Quantity when displayed on the website (example: T1503-25G). Please make sure you enter ONLY the product number in the Product Number field (example: T1503).

Example:

T1503
Product Number
-
25G
Pack Size/Quantity

Additional examples:

705578-5MG-PW

PL860-CGA/SHF-1EA

MMYOMAG-74K-13

1000309185

enter as 1.000309185)

Having trouble? Feel free to contact Technical Service for assistance.

Lot and Batch Numbers can be found on a product's label following the words 'Lot' or 'Batch'.

Aldrich Products

  • For a lot number such as TO09019TO, enter it as 09019TO (without the first two letters 'TO').

  • For a lot number with a filling-code such as 05427ES-021, enter it as 05427ES (without the filling-code '-021').

  • For a lot number with a filling-code such as STBB0728K9, enter it as STBB0728 without the filling-code 'K9'.

Not Finding What You Are Looking For?

In some cases, a COA may not be available online. If your search was unable to find the COA you can request one.

Request COA

Controlling the number of melanopsin-containing retinal ganglion cells by early light exposure
Hong Jie, et al.
Experimental Eye Research, 17-26 (2013)
Does 5-bromo-2'-deoxyuridine (BrdU) disrupt cell proliferation and neuronal maturation in the adult rat hippocampus in vivo?.
Hancock A, et al.
Behavioural Brain Research, 199(2), 218-221 (2009)
Regulation of later neurogenic stages of adult-derived neural stem/progenitor cells by L-type Ca2+ channels.
Teh DB, et al.
Development, Growth & Differentiation, 56(8), 583-594 (2014)
Controlling the number of melanopsin-containing retinal ganglion cells by early light exposure.
Hong J, et al.
Experimental Eye Research, 111, 17-26 (2013)
JAZ (Znf346), a SIRT1-interacting protein protects neurons by stimulating p21 (WAF/CIP1) expression.
Mallick S and D'Mello SR
The Journal of Biological Chemistry, jbc-M114 (2014)

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service