11080725001
Roche
Neuraminidase (Sialidase)
from Vibrio cholerae
Synonym(s):
Salidase
About This Item
Recommended Products
biological source
Vibrio cholerae
Quality Level
form
solution
mol wt
~95 kDa
packaging
pkg of 1 U
manufacturer/tradename
Roche
optimum pH
5.5-6.2
suitability
suitable for ELISA applications
application(s)
life science and biopharma
sample preparation
shipped in
wet ice
storage temp.
2-8°C
Related Categories
General description
Specificity
Application
- to remove cis-acting sialic acids in CHO (chinese hamster ovary) cells
- for deglycosylation studies
Unit Definition
10 mM N-acetyl-neuraminosyl-D-lactose, 50 mM sodium acetate, 4 mM calcium chloride, bovine serum albumin, 100 μg/ml, pH 5.5. The activity is determined by measuring the released D-lactose using the β-galactosidase/galactose dehydrogenase method. Under the same conditions, 1 μmol N-acetylneuraminic acid per min is split off from human acid α1-glycoprotein (10 mg/ml incubation mixture) by 1 U neuraminidase. Released N-acetyl-neuraminic acid can be determined using, for example, the thiobarbituric acid method.
Physical form
Note: The serum used for this preparation was tested for HBs antigen and for the presence of antibodies to HIV-1, HIV-2, HCV, and found to be negative, according to the current quality control procedures.
Other Notes
Legal Information
signalword
Warning
hcodes
Hazard Classifications
Skin Sens. 1
wgk_germany
WGK 1
flash_point_f
does not flash
flash_point_c
does not flash
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Protocols
Neuraminidase can be used to cleave sialic acids from proteins. In this protocol, the enzyme from Vibrio cholerae is used on fixed cells.
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