MilliporeSigma
All Photos(1)

Documents

11299964001

Roche

5-Bromo-2′-deoxy-uridine Labeling and Detection Kit II

sufficient for ≤100 tests, storage temp.:-20°C

Sign Into View Organizational & Contract Pricing

Synonym(s):
5-BrdU, 5-Bromo-2-deoxyuridine

Quality Level

usage

sufficient for ≤100 tests

manufacturer/tradename

Roche

storage temp.

−20°C

Compare Similar Items

View Full Comparison

Show Differences

1 of 4

This Item
B0631B9285B5002
5-Bromo-2′-deoxyuridine BioUltra, ≥99%

B9285

5-Bromo-2′-deoxyuridine

5-Bromo-2′-deoxyuridine ≥99% (HPLC)

B5002

5-Bromo-2′-deoxyuridine

Quality Level

100

Quality Level

-

Quality Level

-

Quality Level

200

usage

sufficient for ≤100 tests

usage

-

usage

-

usage

-

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

General description

Cell proliferation may be studied by monitoring the incorporation of a radioisotope, [ 3H]-thymidine, into cellular DNA, followed by autoradiography.
Alternatively, 5-bromo-2′-deoxy-uridine (BrdU) may be used instead of thymidine. Cells that have incorporated BrdU into DNA are easily detected using a monoclonal antibody against BrdU and an enzyme- or fluorochrome-conjugated second antibody.
Immunohistocytochemical assay for the detection of 5-bromo-2′-deoxy-uridine (BrdU) incorporated into cellular DNA.

Specificity

Anti-BrdU monoclonal antibody specifically binds to 5-bromo-2′-deoxy-uridine, and shows cross-reactivity with 5-iodo-2′-deoxy-uridine (10%). Anti-BrdU shows no cross-reactivity with 5-fluoro-2′-deoxy-uridine or any endogenous cellular component, such as thymidine or uridine.

Application

5-Bromo-2′-deoxy-uridine Labeling and Detection Kit II has been used in:
  • labeling of tooth roots for histology
  • immunostaining of mice frontal sections
  • immunofluorescence imaging of hepatocellular carcinoma sections

The kit is used for the detection of BrdU incorporated into cellular DNA by immunohistocytochemistry.

Features and Benefits

  • Safe: No radioisotopes are used.
  • Easy to perform: Follows a standard immunohistochemistry protocol.
  • Sensitive: Denaturation of DNA with nucleases allows for highly sensitive detection of BrdU.
  • Flexible: Allows double-labeling protocols.

Packaging

1 kit containing 7 components.

Principle

Samples prelabeled with BrdU are fixed with ethanol, then incubated with a monoclonal antibody to BrdU, which contains an optimized mixture of nucleases. These nucleases generate single-stranded DNA fragments that allow binding of the antibody to BrdU. Next, an alkaline phosphatase (AP)-labeled antibody to mouse immunoglobulin is added, then bound to the anti-BrdU antibody. The sample is then incubated with the AP substrate and NBT/ BCIP, which is metabolized to form a colored reaction product. The sample is evaluated using a phase-contrast microscope.

Preparation Note

Working concentration: Working concentration of the labeling reagent corresponds to the WC of the In Situ Cell Proliferation Kits.
Sample material:
Cell culture:
adherent cells, suspension cells, organ or explant cultures. Frozen or paraffin-embedded tissue sections (after in vivo labeling).

Other Notes

For life science research only. Not for use in diagnostic procedures.

Kit Components Only

Product No.
Description

  • BrdU Labeling Reagent 1,000x concentrated

  • Washing Buffer concentrate 10x concentrated

  • Incubation Buffer

  • Anti-BrdU antibody, contains nucleases for DNA denaturation

  • Anti-mouse Ig-alkaline Phosphatase antibody

  • NBT

  • BCIP

signalword

Danger

Hazard Classifications

Acute Tox. 4 - Acute Tox. 4 Dermal - Eye Irrit. 2 - Flam. Liq. 3 Inhalation - Repr. 1B - Skin Sens. 1

Storage Class

3 - Flammable liquids

wgk_germany

WGK 2

flash_point_f

136.4 °F

flash_point_c

58 °C


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Documents related to the products that you have purchased in the past have been gathered in the Document Library for your convenience.

Visit the Document Library

Difficulty Finding Your Product Or Lot/Batch Number?

Product numbers are combined with Pack Sizes/Quantity when displayed on the website (example: T1503-25G). Please make sure you enter ONLY the product number in the Product Number field (example: T1503).

Example:

T1503
Product Number
-
25G
Pack Size/Quantity

Additional examples:

705578-5MG-PW

PL860-CGA/SHF-1EA

MMYOMAG-74K-13

1000309185

enter as 1.000309185)

Having trouble? Feel free to contact Technical Service for assistance.

Lot and Batch Numbers can be found on a product's label following the words 'Lot' or 'Batch'.

Aldrich Products

  • For a lot number such as TO09019TO, enter it as 09019TO (without the first two letters 'TO').

  • For a lot number with a filling-code such as 05427ES-021, enter it as 05427ES (without the filling-code '-021').

  • For a lot number with a filling-code such as STBB0728K9, enter it as STBB0728 without the filling-code 'K9'.

Not Finding What You Are Looking For?

In some cases, a COA may not be available online. If your search was unable to find the COA you can request one.

Request COA

CXCL14 and MCP1 are potent trophic factors associated with cell migration and angiogenesis leading to higher regenerative potential of dental pulp side population cells
Hayashi Y, et al.
Stem Cell Research & Therapy, 6(1), 111-111 (2015)
L J Backman et al.
Scandinavian journal of medicine & science in sports, 23(6), 687-696 (2012-02-02)
The histopathology of tendons with painful tendinopathy is often tendinosis, a fibrosis-like condition of unclear pathogenesis characterized by tissue changes including hypercellularity. The primary tendon cells (tenocytes) have been shown to express adrenoreceptors (mainly alpha-2A) as well as markers of
Pten regulates neural crest proliferation and differentiation during mouse craniofacial development
Yang T, et al.
Developmental Dynamics, 247(2), 304-314 (2018)
Tianfang Yang et al.
Developmental dynamics : an official publication of the American Association of Anatomists, 247(2), 304-314 (2017-11-09)
The phosphatase and tensin homolog deleted on chromosome TEN (Pten) is implicated in a broad range of developmental events and diseases. However, its role in neural crest and craniofacial development has not been well illustrated. Using genetically engineered mouse models
Fenglei He et al.
Development (Cambridge, England), 144(21), 4026-4036 (2017-09-28)
Craniosynostosis is a prevalent human birth defect characterized by premature fusion of calvarial bones. In this study, we show that tight regulation of endogenous PDGFRα activity is required for normal calvarium development in the mouse and that dysregulated PDGFRα activity

Articles

Cell based assays for cell proliferation (BrdU, MTT, WST1), cell viability and cytotoxicity experiments for applications in cancer, neuroscience and stem cell research.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service