11444646001
Roche
Uracil-DNA Glycosylase
recombinant from E. coli K 12
Synonym(s):
PCR, UDG
About This Item
Recommended Products
recombinant
expressed in E. coli
Quality Level
form
solution
packaging
pkg of 100 U
manufacturer/tradename
Roche
concentration
1000 U/mL
technique(s)
PCR: suitable
mutagenesis: suitable
color
colorless
pH
7.9-8.1 (68 °F)
solubility
water: miscible
suitability
suitable for molecular biology
application(s)
life science and biopharma
foreign activity
RNases 10 units, none detected
endonuclease 20 units, none detected
storage temp.
−20°C
Related Categories
General description
Specificity
- Uracil-DNA glycosylase hydrolyzes uracil-glycosidic bonds at U-DNA sites in single- and doublestranded DNA, excising uracil and creating alkali sensitive abasic sites in the DNA.
- The enzyme is more active on single-stranded DNA than on double-standed DNA.
- Activity was also observed on small U-DNA oligonucleotides and on dUMP (Duncan, unpublished observations).
- Uracil-DNA glycosylase is inactive on RNA and native, uracil-free DNA.
Heat inactivation: 95 °C for 10 min
Uracil-DNA glycosylase remains partially active (<10%) after an incubation period of 30 minutes at 95 °C.
Application
- Prevent carryover contamination in PCR
- Increase the efficiency of site-directed mutagenesis procedures
- Label oligonucleotide probes
Quality
Unit Definition
One Lindahl unit is defined as the amount of enzyme necessary to release of 1 μmol uracil at +37 °C in 1 minute. One Lindahl unit is comparable to 520,000 U based on our unit definition.
Volume Activity: 1 U/μl
Physical form
Storage and Stability
Other Notes
wgk_germany
WGK 1
flash_point_f
No data available
flash_point_c
No data available
Certificates of Analysis (COA)
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Articles
DNA damage and repair mechanism is vital for maintaining DNA integrity. Damage to cellular DNA is involved in mutagenesis, the development of cancer among others.
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