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17134

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CPC-Agar (Base)

suitable for microbiology, NutriSelect® Plus

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Synonym(s):
Cellobiose Polymyxin Colistin Agar (Base)
NACRES:
NA.74

sterility

non-sterile

Quality Level

form

powder

composition

agar, 15 g/L
beef extract, 5 g/L
bromothymol blue, 0.04 g/L
cellobiose, 15 g/L
cresol red, 0.04 g/L
peptic digest of animal tissue, 10 g/L
sodium chloride, 20 g/L

manufacturer/tradename

NutriSelect® Plus

technique(s)

microbiological culture: suitable

final pH

7.6±0.2 (25 °C)

application(s)

food and beverages
microbiology

suitability

selective and differential for Vibrio spp.

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suitability

selective and differential for Vibrio spp.

suitability

selective and differential for Clostridium spp.

suitability

nonselective and differential for Enterococcus spp., nonselective and differential for Escherichia coli, nonselective and differential for Klebsiella spp., nonselective and differential for Lactobacillus spp., nonselective and differential for Proteus spp., nonselective and differential for Pseudomonas spp., nonselective and differential for Salmonella spp., nonselective and differential for Staphylococcus spp., nonselective and differential for Streptococcus spp., nonselective and differential for coliforms

suitability

selective and differential for Vibrio spp.

form

powder

form

powder

form

powder

form

powder

composition

agar, 15 g/L , bromothymol blue, 0.04 g/L , cresol red, 0.04 g/L , sodium chloride, 20 g/L , cellobiose, 15 g/L , beef extract, 5 g/L , peptic digest of animal tissue, 10 g/L

composition

agar, 15 g/L , bromocresol purple, 0.04 g/L , L-cysteine hydrochloride, 1 g/L , ferric chloride 6H2O, 0.09 g/L , indoxyl-β-D-glucoside, 0.06 g/L , magnesium sulfate 7H2O, 0.1 g/L , sucrose, 5 g/L , tryptose, 30 g/L , yeast extract, 20 g/L

composition

agar, 15 g/L , beef extract, 3 g/L , bromo thymol blue, 0.02 g/L , casein enzymic hydrolysate, 4 g/L , L-cystine, 0.128 g/L , lactose, 10 g/L , peptic digest of animal tissue, 4 g/L

composition

agar, 14 g/L , bromothymol blue, 0.04 g/L , ferric citrate, 1 g/L , mixed peptone, 10 g/L , ox-bile, 5 g/L , sodium chloride, 10 g/L , sodium cholate, 3 g/L , sodium citrate, 10 g/L , sodium thiosulfate, 10 g/L , sucrose, 20 g/L , yeast extract, 5 g/L

sterility

non-sterile

sterility

non-sterile

sterility

non-sterile

sterility

non-sterile

application(s)

food and beverages, microbiology

application(s)

environmental
food and beverages
water monitoring, microbiology

application(s)

clinical testing
environmental
food and beverages, microbiology

application(s)

environmental
food and beverages, microbiology

General description

Cellobiose-polymyxin B-colistin(CPC) agar is a medium used for the isolation and differentiation of Vibrio vulnificus in laboratory testing.

Application

for the cultivation and identification of Vibrio species from foods

Preparation Note

Suspend 32.54 g in 500 ml of distilled water. Heat to boiling to dissolve the medium completely. Sterilize by autoclaving at 121°C for 15 minutes. Cool to 45°C and aseptically add reconstituted contents of 1 vial of CPC Supplement (Cat. No. 17137). Mix well.

Footnote

We offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

Legal Information

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)


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D-(+)-Cellobiose ≥98% (HPLC)

Sigma-Aldrich

C7252

D-(+)-Cellobiose

4-Aminobenzoic acid ReagentPlus®, ≥99%

Sigma-Aldrich

A9878

4-Aminobenzoic acid

Improved isolation of Vibrio vulnificusfrom seawater and sediment with cellobiose-colistin agar.
H?i L, et al.
Applied and Environmental Microbiology, 64(5), 1721-1724 (1998)

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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