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WL Differential Agar

suitable for microbiology, NutriSelect® Plus

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Synonym(s):
WLD Agar, Wallerstein Differential Agar
NACRES:
NA.74

sterility

non-sterile

Quality Level

form

powder

shelf life

limited shelf life, expiry date on the label

composition

actidione (cycloheximide), 0.004 g/L
agar, 20 g/L
bromocresol green, 0.022 g/L
calcium chloride, 0.125 g/L
casein enzymic hydrolysate, 5 g/L
dextrose, 50 g/L
ferric chloride, 0.0025 g/L
magnesium sulfate, 0.125 g/L
manganese sulfate, 0.0025 g/L
monopotassium phosphate, 0.55 g/L
potassium chloride, 0.425 g/L
yeast extract, 4 g/L

manufacturer/tradename

NutriSelect® Plus

technique(s)

microbiological culture: suitable

final pH

5.5±0.2 (25 °C)

application(s)

bioburden testing
food and beverages

microbiology

suitability

selective and differential for Escherichia coli
selective and differential for Lactobacillus spp.
selective and differential for Proteus spp.
selective and differential for bacteria (General Media)
selective and differential for coliforms

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technique(s)

microbiological culture: suitable

technique(s)

microbiological culture: suitable

technique(s)

microbiological culture: suitable

technique(s)

microbiological culture: suitable

suitability

selective and differential for Escherichia coli, selective and differential for Proteus spp., selective and differential for coliforms, selective and differential for Lactobacillus spp., selective and differential for bacteria (General Media)

suitability

nonselective and differential for Lactobacillus spp., nonselective and differential for Streptococcus spp.

suitability

nonselective and differential for Candida spp., nonselective and differential for Escherichia coli, nonselective and differential for Lactobacillus spp., nonselective and differential for Pichia spp., nonselective and differential for Proteus spp., nonselective and differential for Saccharomyces spp., nonselective and differential for Zygosaccharomyces spp., nonselective and differential for bacteria (General Media), nonselective and differential for coliforms, nonselective and differential for molds (General Media), nonselective and differential for yeasts (General Media)

suitability

selective and differential for Listeria spp.

sterility

non-sterile

sterility

non-sterile

sterility

non-sterile

sterility

non-sterile

composition

actidione (cycloheximide), 0.004 g/L , bromocresol green, 0.022 g/L , casein enzymic hydrolysate, 5 g/L , ferric chloride, 0.0025 g/L , manganese sulfate, 0.0025 g/L , potassium chloride, 0.425 g/L , agar, 20 g/L , dextrose, 50 g/L , monopotassium phosphate, 0.55 g/L , calcium chloride, 0.125 g/L , yeast extract, 4 g/L , magnesium sulfate, 0.125 g/L

composition

agar, 15 g/L , beef extract, 5 g/L , casein enzymic hydrolysate, 10 g/L , L-cysteine hydrochloride, 0.3 g/L , dextrose, 20 g/L , papaic digest of soybean meal, 5 g/L , sodium chloride, 5 g/L , yeast extract, 5 g/L

composition

agar, 20 g/L , bromocresol green, 0.022 g/L , calcium chloride, 0.125 g/L , casein enzymic hydrolysate, 5 g/L , dextrose, 50 g/L , ferric chloride, 0.0025 g/L , magnesium sulfate, 0.125 g/L , manganese sulfate, 0.0025 g/L , monopotassium phosphate, 0.55 g/L , potassium chloride, 0.425 g/L , yeast extract, 4 g/L

composition

agar, 15.0 g/L , casein enzymic hydrolysate, 6.0 g/L , chromogenic substrate, 0.05 g/L , disodium hydrogen phosphate anhydrous, 2.5 g/L , glucose, 2.0 g/L , lithium chloride, 10.0 g/L , magnesium glycerophosphate, 1.0 g/L , magnesium sulfate, 0.5 g/L , meat peptone, 18.0 g/L , sodium chloride, 5.0 g/L , sodium pyruvate, 2.0 g/L , yeast extract, 10.0 g/L

application(s)

bioburden testing
food and beverages, microbiology

application(s)

bioburden testing
environmental
food and beverages, microbiology

application(s)

bioburden testing
food and beverages, microbiology

application(s)

agriculture
environmental
food and beverages, microbiology

General description

WL (Wallerstein Laboratory) Differential Agar, modified is formulated as described by Green and Gray for the examination of materials encountered in brewing and for industrial fermentations containing mixed flora of yeasts and bacteria. WL Differential Agar helps in selective isolation and enumeration of bacteria encountered in breweries and industrial fermentations.

Application

WL Differential Agar has been used for the enumeration of Kluyveromyces marxianus culture.

Preparation Note

Suspend 80.26 g in 1 litre distilled water. Boil to dissolve the medium completely. Sterilize by autoclaving at 121°C for 15 minutes. If desired, to obtain a pH of 6.5, add 1% solution of sodium bicarbonate.

Footnote

We offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

Legal Information

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)


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A differential procedure for bacteriological studies useful in the fermentation industry
Green SR and Gray PP
Archives of Biochemistry and Biophysics, 32(1), 59-69 (1951)
Interactions between Kluyveromyces marxianus and Saccharomyces cerevisiae in tequila must type medium fermentation
Lopez CLF, et al.
World Journal of Microbiology & Biotechnology, 30(8), 2223-2229 (2014)

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