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Cereus Selective Agar

NutriSelect® Basic, suitable for microbiology

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Synonym(s):
Bacillus Cereus Agar, Bacillus Cereus Selective Agar, Bacillus cereus Egg yolk Polymyxin Agar Base, Cereus Selective Agar Base acc. to Mossel, MYP Agar, Mannitol-Egg-yolk-polymyxine-Agar, PEMBA, PREP agar, Polymixin Pyruvate Egg yolk Mannitol Bromothymol blue Agar
NACRES:
NA.74

sterility

non-sterile

Quality Level

form

powder

shelf life

limited shelf life, expiry date on the label

composition

agar, 12 g/L
D(-)-mannitol, 10 g/L
meat extract, 1 g/L
meat peptone, 10 g/L
phenol red, 0.025 g/L
sodium chloride, 10 g/L

manufacturer/tradename

NutriSelect® Basic

technique(s)

microbiological culture: suitable

final pH

7.1±0.2 (25 °C)

application(s)

clinical testing
environmental
food and beverages

microbiology

suitability

selective and differential for Bacillus cereus
selective and differential for Bacillus spp.

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suitability

selective and differential for Bacillus cereus, selective and differential for Bacillus spp.

suitability

selective and differential for Klebsiella spp.

suitability

nonselective and differential for Enterobacter spp., nonselective and differential for Escherichia coli, nonselective and differential for Klebsiella spp., nonselective and differential for Proteus spp., nonselective and differential for Yersinia spp., nonselective and differential for coliforms

suitability

selective and differential for Staphylococcus spp.

technique(s)

microbiological culture: suitable

technique(s)

-

technique(s)

microbe id | specific enzyme detection: suitable, microbiological culture: suitable

technique(s)

-

composition

agar, 12 g/L , meat extract, 1 g/L , phenol red, 0.025 g/L , D(-)-mannitol, 10 g/L , sodium chloride, 10 g/L , meat peptone, 10 g/L

composition

agar, 15.0 g/L , bile salts mixture, 1.5 g/L , chromogenic mixture, 0.2 g/L , petone, special, 12.0 g/L , sodium chloride, 5.0 g/L , sodium lauryl sulfate, 0.1 g/L , yeast extract, 7.0 g/L

composition

agar, 12 g/L , gelatine peptone, 1 g/L , D(+)-glucose, 1 g/L , phenol red, 0.012 g/L , potassium dihydrogen phosphate, 2 g/L , sodium chloride, 5 g/L

composition

agar, 15.0 g/L , beef extract, 2.5 g/L , casein enzymic hydrolysate, 13.0 g/L , chromogenic mixture, 5.3 g/L , sodium chloride, 40.0 g/L , sodium pyruvate, 5.0 g/L , yeast extract, 2.5 g/L

sterility

non-sterile

sterility

non-sterile

sterility

non-sterile

sterility

non-sterile

application(s)

clinical testing
environmental
food and beverages, microbiology

application(s)

bioburden testing
clinical testing
environmental
food and beverages
water monitoring, microbiology

application(s)

clinical testing
environmental
food and beverages, microbiology

application(s)

clinical testing
food and beverages, microbiology

Application

For enumeration, identification and isolation of Bacillus cereus in food.

Preparation Note

Dissolve 43 g in 900 ml water. Sterilize by autoclaving at 121°C for 15 minutes and let cool to 45-50°C. Add 100 ml egg-yolk suspension (Cat. No. 17148) and 10 mg polymyxin B (Cat. No. 81334) or 2 vial/l Polymyxin B Selective Supplement (Cat. No. P9602) to the autoclaved solution.

Footnote

We offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

Legal Information

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class

11 - Combustible Solids

wgk_germany

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable


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Customers Also Viewed

Slide 1 of 3

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D A Mossel et al.
Applied microbiology, 15(3), 650-653 (1967-05-01)
For the enumeration of vegetative cells and spores of Bacillus cereus in foods, a mannitol-egg yolk-phenol red-agar has been developed which exploits the failure of B. cereus to dissimilate mannitol, and the ability of most strains to produce phospholipase C.

Articles

Microbiological assessment of bacteria found in spices, a potent source for food spoilage and pathogens.

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