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41960

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Vegitone infusion broth

suitable for microbiology, NutriSelect® Plus

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Synonym(s):
Brain Heart Broth, Vegitone
NACRES:
NA.85

sterility

non-sterile

Quality Level

form

powder

shelf life

limited shelf life, expiry date on the label

composition

dextrose, 2.0 g/L
disodium phosphate, 2.5 g/L
peptone (vegetable), 10.0 g/L
sodium chloride, 5.0 g/L
vegetable infusion powder, 5.0 g/L
vegetable special infusion powder, 12.5 g/L

manufacturer/tradename

NutriSelect® Plus

packaging

pkg of -500 g

storage condition

(Dry place, tightly sealed containers)

final pH

7.4±0.2 (25 °C)

application(s)

agriculture
clinical testing
environmental
food and beverages
life science and biopharma
sterility testing

microbiology

storage temp.

2-25°C

suitability

Haemophilus spp.
nonselective for Bacteroides spp.
nonselective for Candida spp.
nonselective for Escherichia coli
nonselective for Neisseria spp.
nonselective for Pseudomonas spp.
nonselective for Staphylococcus spp.
nonselective for Streptococcus spp.
nonselective for bacteria (General Media)
nonselective for coliforms

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suitability

Haemophilus spp., nonselective for Candida spp., nonselective for Neisseria spp., nonselective for Staphylococcus spp., nonselective for bacteria (General Media), nonselective for Bacteroides spp., nonselective for Pseudomonas spp., nonselective for coliforms, nonselective for Escherichia coli, nonselective for Streptococcus spp.

suitability

nonselective for Bacillus spp., nonselective for Bacteroides spp., nonselective for Campylobacter spp., nonselective for Candida spp., nonselective for Escherichia coli, nonselective for Neisseria spp., nonselective for Staphylococcus spp., nonselective for Streptococcus spp., nonselective for bacteria (General Media), nonselective for coliforms

suitability

nonselective for bacteria (General Media)

suitability

nonselective for Escherichia coli, nonselective for Pseudomonas spp., nonselective for Staphylococcus spp., nonselective for Streptococcus spp., nonselective for bacteria (General Media), nonselective for coliforms

form

powder

form

powder

form

powder

form

powder

sterility

non-sterile

sterility

non-sterile

sterility

non-sterile

sterility

non-sterile

final pH

7.4±0.2 (25 °C)

final pH

7.3±0.2 (25 °C)

final pH

7.4±0.2 (25 °C)

final pH

7.4±0.2 (25 °C)

packaging

pkg of -500 g

packaging

-

packaging

-

packaging

-

General description

Vegitone Infusion Broth is like Brain Heart Infusion Broth with the difference that animal peptones are replaced by plant peptones. It is a highly nutritious and well-buffered medium that supports the growth of a wide variety of microorganisms. Peptone (vegetable), Vegetable Special Infusion powder, and Vegetable Infusion powder are non-animal nutritive sources of peptides and amino acids providing nitrogen for the growth of microorganisms. Dextrose is the fermentable carbohydrate source, and sodium chloride is for the osmotic balance in the media. Disodium phosphate is used as the buffering agent.The addition of desired components allows the modification of this medium as per cultivation requirements. Sodium chloride (6.5 %) makes the medium selective for salt-tolerant bacteria like Enterococci. Adding agar (0.1 %) to the broth improves the growth of micro-aerophilic and anaerobic microorganisms. Enrichment with 10 % sheep blood allows isolation and cultivation of a wide variety of fungal species, including systemic fungi from clinical specimens. Additional gentamicin and/or chloramphenicol accommodate for selective isolation of these fungi.

Application

Vegitone Infusion Broth is employed for the propagation of fastidious pathogenic cocci and other organisms associated with blood culture work and allied pathological investigations. This medium is also suitable for inoculum preparation used in antimicrobial susceptibility tests. It is also recommended by ISO Committee under the specifications ISO 6888:1983 for the enrichment of Staphylococcus aureus.

Preparation Note

Suspend 37 g in 1000 ml distilled water. Dispense into bottles or tubes and sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. For best results, the medium should be used on the day it is prepared, otherwise, it should be boiled or steamed for a few minutes and then cooled before use.

Footnote

We offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

Legal Information

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)


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