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ONPG Disks

suitable for microbiology

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Synonym(s):
β-Galactosidase Test Disks, 2-Nitrophenyl-β-D-galactopyranoside Disks
NACRES:
NA.85

agency

according to GB 4789.4-2016

Quality Level

product line

BioChemika

shelf life

limited shelf life, expiry date on the label

technique(s)

microbe id | specific enzyme detection: suitable

application(s)

environmental
food and beverages

microbiology

shipped in

wet ice

storage temp.

2-8°C

suitability

Escherichia coli
bacteria

General description

ONPG Disks are used to detect the presence of β-galactosidase, an enzyme found in lactose-fermenting organisms. Lactose utilization depends upon two enzymes: β-galactoside permease, which catalyzes transport of lactose into the cell, and β-galactosidase, which breaks down lactose into galactose and glucose. β-Galactosidase is not lactose specific and can act on simple galactosides including the ONPG (o-nitrophenyl-β-D-galactopyranose) substrate. ONPG hydrolysis results in the release of galactose, and the yellow chromogenic compound, o-nitrophenol. The test substrate, ONPG, does not depend on an induced or constitutive permease enzyme to enter the cell, therefore reactions are rapid and occur within a 24-hour period even for late lactose fermenters.

Packaging

Sterile filter paper discs (50 pcs, diameter 6 mm) impregnated with o-nitrophenyl-β-D-galactopyranose

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)


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