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Kovac′s reagent for indoles

suitable for microbiology

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Synonym(s):
4-(Dimethylamino)benzaldehyde solution
Empirical Formula (Hill Notation):
C9H11NO
CAS Number:
Molecular Weight:
149.19
Beilstein:
4132845
MDL number:
PubChem Substance ID:
NACRES:
NA.85

Agency

according to ISO 16654:2001

Quality Level

product line

BioChemika

shelf life

limited shelf life, expiry date on the label

composition

4-(dimethlyamino)benzaldehyde, 50 g/L
hydrochloric acid, 240 g/L
isoamylic alcohol, 710 g/L

technique(s)

microbe id | specific enzyme detection: suitable

application(s)

agriculture
clinical testing
environmental
food and beverages

microbiology

storage temp.

2-8°C

suitability

Escherichia coli
coliforms

SMILES string

[H]C(=O)c1ccc(cc1)N(C)C

InChI

1S/C9H11NO/c1-10(2)9-5-3-8(7-11)4-6-9/h3-7H,1-2H3

InChI key

BGNGWHSBYQYVRX-UHFFFAOYSA-N

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1 of 4

This Item
609831.0929378719
Millipore

Millipore

1.09293

KOVACS′ indole reagent

Millipore

Millipore

78719

Kovac′s Reagent Strips

Quality Level

200

Quality Level

100

Quality Level

200

Quality Level

100

agency

according to ISO 16654:2001

agency

-

agency

-

agency

-

product line

BioChemika

product line

-

product line

-

product line

-

shelf life

limited shelf life, expiry date on the label

shelf life

limited shelf life, expiry date on the label

shelf life

-

shelf life

limited shelf life, expiry date on the label

composition

4-(dimethlyamino)benzaldehyde, 50 g/L

composition

n-butanol , 4-dimethylaminobenzaldehyde , hydrochloric acid

composition

-

composition

-

General description

Kovac′s reagent is prepared by mixing p-dimethylaminobenzaldehyde, isoamyl alcohol and concentrated hydrochloric acid. For identification of an organism, the formation of Indole from a tryptophan substrate is a useful diagnostic tool. Indole production is a crucial test in identification of Escherichia coli. After incubation, adding the reagent will help in determination if Indole that has been produced. The reagent turns red when reacts with Indole.

Application

In the presence of oxygen, some bacteria, like E.coli, are able to split tryptophan into indole and alpha-aminopropionic acid. This reagent is for detecting the indole and identify the indole-positive and indole-negative microorganisms.
Kovac′s reagent may be used for spot test for determination of indole by saturating filter paper with the reagent. It may also be used for initial spot test for determination of indole followed by quantification by spectrophotometric assay and HPLC-UV/Vis-MS/MS methods.

replaced by

Product No.
Description
Pricing

Signal Word

Danger

Hazard Classifications

Acute Tox. 4 Inhalation - Eye Dam. 1 - Flam. Liq. 3 - Met. Corr. 1 - Skin Corr. 1B - Skin Sens. 1 - STOT SE 3

Target Organs

Respiratory system

Storage Class Code

3 - Flammable liquids

WGK

WGK 1

Flash Point(F)

109.4 °F

Flash Point(C)

43 °C

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Rita R. Colwell, R. Grigorova
Methods in Microbiology, 19, 27-27 (1987)
S. Harisha
An Introduction to Practical Biotechnology, 193-193 (2005)
Patrick R Porubsky et al.
Archives of biochemistry and biophysics, 475(1), 14-17 (2008-04-22)
Cytochrome P450 2A13 (CYP2A13) is a lung specific enzyme known to activate the potent tobacco procarcinogen 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone (NNK) into two carcinogenic metabolites. CYP2A13 has been crystallized and X-ray diffraction experiments illuminated the structure of this enzyme, but with an unknown
R W Trepeta et al.
Journal of clinical microbiology, 19(2), 172-174 (1984-02-01)
Escherichia coli is the most common gram-negative microbe isolated and identified in clinical microbiology laboratories. It can be identified within 1 h by oxidase, indole, lactose, and beta-glucuronidase tests. The oxidase and indole tests are performed as spot tests, and
G P Kushto et al.
Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy, 54A(6), 799-819 (1998-07-17)
Results of a normal coordinate analysis based on infrared and Raman spectra of six isotopic forms of 4-(dimethylamino)benzaldehyde are reported (DABA, DABA-CDO, DABA-13CDO, DABA-13CHO, DABA-CHO18O, and DABA-3,5-D). Cs point group symmetry has been applied and all motions except the internal

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