77500

1,10-Phenanthroline monohydrate

for the spectrophotometric determination of Fe, Pd, V, ≥99.0%

• Synonym(s): o-Phenanthroline monohydrate
• Empirical Formula (Hill Notation): C₁₂H₈N₂ · H₂O
• CAS Number: 5144-89-8
• Molecular Weight: 198.22
• Beilstein/REAXYS Number: 4008096
• EC Number: 200-629-2
• MDL number: MFCD00149973
• UNSPSC Code: 12000000
• PubChem Substance ID: 329767727
• NACRES: NA.21

Properties

• Quality Level: 100
• assay: ≥99.0% (HPLC); ≥99.0% (NT); ≥99.0%
• form: crystals
• quality: for the spectrophotometric determination of Fe, Pd, V
• technique(s): UV/Vis spectroscopy: suitable; titration: suitable
• ign. residue: ≤0.05%
• mp: 100-104 °C (lit.); 99-104 °C
• cation traces: Ca: ≤50 mg/kg; Cd: ≤20 mg/kg; Co: ≤20 mg/kg; Cr: ≤20 mg/kg; Cu: ≤20 mg/kg; Fe: ≤20 mg/kg; K: ≤100 mg/kg; Mg: ≤20 mg/kg; Mn: ≤20 mg/kg; Na: ≤100 mg/kg; Ni: ≤20 mg/kg; Pb: ≤20 mg/kg; Zn: ≤20 mg/kg
• SMILES string: O.c1cnc2c(c1)ccc3cccnc23
• InChI: 1S/C12H8N2.H2O/c1-3-9-5-6-10-4-2-8-14-12(10)11(9)13-7-1;/h1-8H;1H2
• InChI key: PPQJCISYYXZCAE-UHFFFAOYSA-N

Description

General description

1,10-Phenanthroline monohydrate is a chelating agent.

Application

It has been used in preparing 0.1 % phenanthroline solution. In another study, 80μl of 1,10-Phenanthroline monohydrate was used along with nanoparticles to measure iron content using colorimetric assay.3 It may be used in the preparation of [Mn(1,10-phen)₂(mal)].₂H₂O.

When complexed with copper, it possesses nuclease activity that has been used to study DNA-protein interactions.

Biochem/physiol Actions

Metalloprotease inhibitor; chelates iron, zinc and other divalent metals. Effective concentration 1-10 mM.

Safety Information

• pictograms: GHS06,GHS09
• signalword: Danger
• hcodes: H301,H410
• pcodes: P264 - P273 - P301 + P310 - P391 - P405 - P501
• Hazard Classifications: Acute Tox. 3 Oral - Aquatic Acute 1 - Aquatic Chronic 1
• Storage Class: 6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
• wgk_germany: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Faceshields, Gloves, type P2 (EN 143) respirator cartridges